Application Data
(Histone H3 phospho Ser28 antibody specificity tested by peptide array analysis. Peptide array analysis was used to confirm the specificity of this antibody for its intended modification. Histone H3 phospho Ser28 antibody was applied at a dilution of 1:3,000 to MODified Histone Peptide Array. The arrays were scanned with ArrayAnalysis Software 7 and the results plotted. Specificity data is shown for the most reactive peptides and those related to the immunogen. Recognition of the Histone H3 phospho Ser28 peptides by the antibody is inhibited by Lys27 monomethylation and blocked by Arg26 dimethylation and a citrulline at position 26. Array Data File)
Rat Histone H3S28ph Monoclonal Antibody | anti-H3S28ph antibody
Histone H3S28ph antibody (mAb)(Clone HTA28)
Reactivity
Human, Mouse, Rat, Wide Range Predicted
Applications
Immunohistochemistry, Immunofluorescence
Purity
Affinity Purified
Synonyms
Histone H3S28ph, Antibody; Histone H3S28ph antibody (mAb)(Clone HTA28); anti-H3S28ph antibody
Host
Rat
Reactivity
Human, Mouse, Rat, Wide Range Predicted
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
HTA28
Purity/Purification
Affinity Purified
Form/Format
Purified IgG in 0.1 M Tris-glycine (pH7.4), 150mM NaCl and 0.05% sodium azide. Sodium azide is highly toxic. For your convenience, an ascites version of this antibody is also available.
Concentration
1.15ug/ul (varies by lot)
Applicable Applications for anti-H3S28ph antibody
IHC (Immunohistochemistry), IF (Immunofluorescence)
Conjugation
Phosphorylated
Immunogen
This Histone H3 phospho Ser28 antibody was raised in rat against a peptide corresponding to amino acid residues 23-35 (phospho Ser 28) of human Histone H3.
Abs Residue
Ser
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Application Data
(Histone H3 phospho Ser28 antibody specificity tested by peptide array analysis. Peptide array analysis was used to confirm the specificity of this antibody for its intended modification. Histone H3 phospho Ser28 antibody was applied at a dilution of 1:3,000 to MODified Histone Peptide Array. The arrays were scanned with ArrayAnalysis Software 7 and the results plotted. Specificity data is shown for the most reactive peptides and those related to the immunogen. Recognition of the Histone H3 phospho Ser28 peptides by the antibody is inhibited by Lys27 monomethylation and blocked by Arg26 dimethylation and a citrulline at position 26. Array Data File)
Application Data
(Histone H3 phospho Ser28 antibody specificity tested by peptide array analysis. Peptide array analysis was used to confirm the specificity of this antibody for its intended modification. Histone H3 phospho Ser28 antibody was applied at a dilution of 1:3,000 to MODified Histone Peptide Array. The arrays were scanned with ArrayAnalysis Software 7 and the results plotted. Specificity data is shown for the most reactive peptides and those related to the immunogen. Recognition of the Histone H3 phospho Ser28 peptides by the antibody is inhibited by Lys27 monomethylation and blocked by Arg26 dimethylation and a citrulline at position 26. Array Data File)
IF (Immunofluorescence)
(Histone H3 phospho Ser28 antibody tested by immunofluorescence. Top: HeLa cells stained with Histone H3 phospho Ser28 antibody at a 1:1,000 dilution. Middle: Same cells stained with DAPI. Bottom: Merge of both images.)
IF (Immunofluorescence)
(Histone H3 phospho Ser28 antibody tested by immunofluorescence. Top: HeLa cells stained with Histone H3 phospho Ser28 antibody at a 1:1,000 dilution. Middle: Same cells stained with DAPI. Bottom: Merge of both images.)
Related Product Information for anti-H3S28ph antibody
Short Description: Our Histone H3S28ph antibody (mAb)(Clone HTA28) was raised in a Rat host. It has been validated for use in Immunofluorescence and Immunohistochemistry; it has been shown to react with Human, Mouse and Rat samples, but it is predicted that it will react with a wide range of sample types.
Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Ser10 phosphorylation and Ser28 phosphorylation in the tail of H3 have very similar kinetics. Both phosphorylations occur early in mitosis when chromosomes begin to condense and during premature chromosome condensation induced in S-phase cells. These phosphorylated serines are excellent mitotic markers. In contrast to Ser10 phosphorylation, Ser28 phosphorylation has never been observed in interphase.
Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Ser10 phosphorylation and Ser28 phosphorylation in the tail of H3 have very similar kinetics. Both phosphorylations occur early in mitosis when chromosomes begin to condense and during premature chromosome condensation induced in S-phase cells. These phosphorylated serines are excellent mitotic markers. In contrast to Ser10 phosphorylation, Ser28 phosphorylation has never been observed in interphase.
Product Categories/Family for anti-H3S28ph antibody
NCBI and Uniprot Product Information
Molecular Weight
17kDa
Similar Products
Product Notes
The H3S28ph (Catalog #AAA59825) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Histone H3S28ph antibody (mAb)(Clone HTA28) reacts with Human, Mouse, Rat, Wide Range Predicted and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H3S28ph can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IF (Immunofluorescence). Researchers should empirically determine the suitability of the H3S28ph for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H3S28ph, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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