Application Data
(Histone H4K20me2 antibody (mAb) specificity tested by peptide array analysis. Peptide array analysis was used to confirm the specificity of this antibody for its intended modification. Histone H4K20me2 antibody (mAb) was applied at a dilution of 0.5 ug/ml to MODified Histone Peptide Array tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H4K20me2 antibody (mAb) for dimethyl-Lys20 of histone H4. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with Histone H4K20me2 antibody (mAb) at 2 ug/ml. The amount of peptide (picomoles) spotted is indicated next to each row. Top panel: Lane 1: unmodified Lys79 peptide Lane 2: monomethyl-Lys79 H4 peptide Lane 3: dimethyl-Lys79 H4 peptide Lane 4: trimethyl-Lys79 H4 peptide Lane 5: monomethyl-Lys20 H4 peptide Lane 6: dimethyl-Lys20 H4 peptide Lane 7: trimethyl-Lys20 H4 peptide Lane 8: acetyl-Lys20 peptide Lane 9: unmodified Lys20 peptide Bottom panel: Lane 1: monomethyl-Lys31 H4 peptide Lane 2: dimethyl-Lys31 H4 peptide Lane 3: trimethyl-Lys31 H4 peptide Lane 4: monomethyl-Lys44 H4 peptide Lane 5: dimethyl-Lys44 H4 peptide Lane 6: trimethyl-Lys44 H4 peptide.)
Mouse Histone H4K20me2 Monoclonal Antibody | anti-H4K20me2 antibody
Histone H4K20me2 antibody (mAb)(Clone MABI 0422)
Gene Names
HIST4H4; H4/p; HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; HIST2H4A; HIST2H4B; MGC24116
Reactivity
Human, Wide Range Predicted
Applications
Western Blot, Dot Blot
Purity
Protein G Chromatography
Synonyms
Histone H4K20me2, Antibody; Histone H4K20me2 antibody (mAb)(Clone MABI 0422); anti-H4K20me2 antibody
Host
Mouse
Reactivity
Human, Wide Range Predicted
Clonality
Monoclonal
Isotype
IgG1
Clone Number
MABI 0422
Purity/Purification
Protein G Chromatography
Form/Format
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-H4K20me2 antibody
WB (Western Blot), DB (Dot Blot)
Conjugation
Methylated
Immunogen
This antibody was raised against a synthetic peptide containing dimethyl-lysine 20 of human Histone H4.
Abs Residue
Lys
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Application Data
(Histone H4K20me2 antibody (mAb) specificity tested by peptide array analysis. Peptide array analysis was used to confirm the specificity of this antibody for its intended modification. Histone H4K20me2 antibody (mAb) was applied at a dilution of 0.5 ug/ml to MODified Histone Peptide Array tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H4K20me2 antibody (mAb) for dimethyl-Lys20 of histone H4. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with Histone H4K20me2 antibody (mAb) at 2 ug/ml. The amount of peptide (picomoles) spotted is indicated next to each row. Top panel: Lane 1: unmodified Lys79 peptide Lane 2: monomethyl-Lys79 H4 peptide Lane 3: dimethyl-Lys79 H4 peptide Lane 4: trimethyl-Lys79 H4 peptide Lane 5: monomethyl-Lys20 H4 peptide Lane 6: dimethyl-Lys20 H4 peptide Lane 7: trimethyl-Lys20 H4 peptide Lane 8: acetyl-Lys20 peptide Lane 9: unmodified Lys20 peptide Bottom panel: Lane 1: monomethyl-Lys31 H4 peptide Lane 2: dimethyl-Lys31 H4 peptide Lane 3: trimethyl-Lys31 H4 peptide Lane 4: monomethyl-Lys44 H4 peptide Lane 5: dimethyl-Lys44 H4 peptide Lane 6: trimethyl-Lys44 H4 peptide.)
Application Data
(Histone H4K20me2 antibody (mAb) specificity tested by peptide array analysis. Peptide array analysis was used to confirm the specificity of this antibody for its intended modification. Histone H4K20me2 antibody (mAb) was applied at a dilution of 0.5 ug/ml to MODified Histone Peptide Array tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H4K20me2 antibody (mAb) for dimethyl-Lys20 of histone H4. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with Histone H4K20me2 antibody (mAb) at 2 ug/ml. The amount of peptide (picomoles) spotted is indicated next to each row. Top panel: Lane 1: unmodified Lys79 peptide Lane 2: monomethyl-Lys79 H4 peptide Lane 3: dimethyl-Lys79 H4 peptide Lane 4: trimethyl-Lys79 H4 peptide Lane 5: monomethyl-Lys20 H4 peptide Lane 6: dimethyl-Lys20 H4 peptide Lane 7: trimethyl-Lys20 H4 peptide Lane 8: acetyl-Lys20 peptide Lane 9: unmodified Lys20 peptide Bottom panel: Lane 1: monomethyl-Lys31 H4 peptide Lane 2: dimethyl-Lys31 H4 peptide Lane 3: trimethyl-Lys31 H4 peptide Lane 4: monomethyl-Lys44 H4 peptide Lane 5: dimethyl-Lys44 H4 peptide Lane 6: trimethyl-Lys44 H4 peptide.)
WB (Western Blot)
(Histone H4K20me2 antibody (mAb) tested by Western blot. Nuclear extract (20 ug) of HeLa cells probed with the Histone H4K20me2 antibody (mAb) at 2 ug/ml.)
WB (Western Blot)
(Histone H4K20me2 antibody (mAb) tested by Western blot. Nuclear extract (20 ug) of HeLa cells probed with the Histone H4K20me2 antibody (mAb) at 2 ug/ml.)
Related Product Information for anti-H4K20me2 antibody
Short Description: Our Histone H4K20me2 antibody (mAb)(Clone MABI 0422) was raised in a Mouse host. It has been validated for use in Dot blot and Western blot; it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.
Background: Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points; it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; they play a major role in regulating gene expression. Histone 4 lysine 20 (H4K20) can be mono-, di- or trimethylated by different histone methyltransferases such as NSD1 or ASH1. The methylation of this lysine is often associated with transcriptional repression.
Background: Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points; it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; they play a major role in regulating gene expression. Histone 4 lysine 20 (H4K20) can be mono-, di- or trimethylated by different histone methyltransferases such as NSD1 or ASH1. The methylation of this lysine is often associated with transcriptional repression.
Product Categories/Family for anti-H4K20me2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11,367 Da
NCBI Official Full Name
histone H4
NCBI Official Synonym Full Names
histone cluster 4, H4
NCBI Official Symbol
HIST4H4
NCBI Official Synonym Symbols
H4/p; HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; HIST2H4A; HIST2H4B; MGC24116
NCBI Protein Information
histone H4; histone 4, H4; OTTHUMP00000239323
UniProt Protein Name
Histone H4
UniProt Gene Name
HIST1H4A
UniProt Synonym Gene Names
H4/A; H4FA
UniProt Entry Name
H4_HUMAN
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Product Notes
The H4K20me2 hist1h4a (Catalog #AAA60046) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Histone H4K20me2 antibody (mAb)(Clone MABI 0422) reacts with Human, Wide Range Predicted and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H4K20me2 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), DB (Dot Blot). Researchers should empirically determine the suitability of the H4K20me2 hist1h4a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H4K20me2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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