ELISA
(Figure 2 ELISA Validation of HIV-1 p24 Antibody with HIV-1 p24 Protein Coating Antigen: HIV-1 p24 recombinant protein, 1ug/mL, incubated at 4 degree C overnight. Detection Antibodies: HIV-1 p24 antibody, PM-6333, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.)
Mouse anti-Virus HIV-1 p24 Monoclonal Antibody | anti-HIV-1 antibody
HIV-1 p24 Antibody [8A1A6](biotin)
Reactivity
Virus
Applications
Western Blot, ELISA
Purity
Anti-HIV-1 p24 antibody is immunoaffinity chromatography purified IgG.
Synonyms
HIV-1 p24, Antibody; HIV-1 p24 Antibody [8A1A6](biotin); Anti-HIV-1 p24 antibody: Pr55Gag; Gag polyprotein; Capsid protein p24; gag Pr55(Gag); anti-HIV-1 antibody
Host
Mouse
Reactivity
Virus
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
8A1A6
Purity/Purification
Anti-HIV-1 p24 antibody is immunoaffinity chromatography purified IgG.
Form/Format
Liquid; HIV-1 p24 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration
1mg/mL (varies by lot)
Applicable Applications for anti-HIV-1 antibody
WB (Western Blot), ELISA
Immunogen
Mouse monoclonal HIV-1 p24 antibody was raised against a recombinant full-length HIV-1 p24 protein.
Research Area
Infectious Disease
NCBI Organism
hiv-1
Conjugation
Biotin
Preparation and Storage
HIV-1 p24 Monoclonal Antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
ELISA
(Figure 2 ELISA Validation of HIV-1 p24 Antibody with HIV-1 p24 Protein Coating Antigen: HIV-1 p24 recombinant protein, 1ug/mL, incubated at 4 degree C overnight. Detection Antibodies: HIV-1 p24 antibody, PM-6333, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.)
ELISA
(Figure 2 ELISA Validation of HIV-1 p24 Antibody with HIV-1 p24 Protein Coating Antigen: HIV-1 p24 recombinant protein, 1ug/mL, incubated at 4 degree C overnight. Detection Antibodies: HIV-1 p24 antibody, PM-6333, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.)
WB (Western Blot)
(Figure 1 Western Blot Validation with HIV-1 p24 Protein Loading: 30 ng HIV-1 p24 recombinant protein. Antibodies: HIV-1 p24, PM-6333, 0.5ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5,000 dilution.)
WB (Western Blot)
(Figure 1 Western Blot Validation with HIV-1 p24 Protein Loading: 30 ng HIV-1 p24 recombinant protein. Antibodies: HIV-1 p24, PM-6333, 0.5ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5,000 dilution.)
Related Product Information for anti-HIV-1 antibody
HIV-1 p24 Monoclonal Antibody: The human immunodeficiency virus type 1 (HIV-1) particle consists of an envelope, a core, and the region between the two termed matrix (1). The HIV-1 Gag protein is a late structural protein that contains four proteins: matrix (p17), capsid (p24), nucleocapsid (p7) and the p6 protein (2). The p24 constitutes the major core component of the virus and shows high degree of sequence conservation among HIV isolates. The Gag p24 protein has been used as an integral part of multicomponent HIV-1 vaccines (3).
Product Categories/Family for anti-HIV-1 antibody
References
Goto et al. Micron 1998; 29:123-38.
Freed EO. Virology 1998; 251:1-15.
Freed EO. Virology 1998; 251:1-15.
Freed EO. Virology 1998; 251:1-15.
Freed EO. Virology 1998; 251:1-15.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
Predicted: 24; 41; 55 kDa
NCBI Official Full Name
gag polyprotein
NCBI Official Symbol
gag
NCBI Protein Information
Pr55(Gag); matrix; capsid; nucleocapsid; p2; p6; p1
UniProt Protein Name
Gag polyprotein
UniProt Gene Name
gag
UniProt Synonym Gene Names
MA; CA; NC
UniProt Entry Name
GAG_HV1H2
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Product Notes
The HIV-1 gag (Catalog #AAA40996) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The HIV-1 p24 Antibody [8A1A6](biotin) reacts with Virus and may cross-react with other species as described in the data sheet. AAA Biotech's HIV-1 p24 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the HIV-1 gag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HIV-1 p24, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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