Mouse HLA-A Monoclonal Antibody | anti-HLA-A antibody

HLA-A Antibody (Center)

Cat. Num. AAA283464

• Gene Names: HLA-A; HLAA
• Reactivity: Mouse (Predicted Reactivity: Human)
• Applications: ELISA, Western Blot
• Purity: This antibody is purified through a protein G column, followed by dialysis against PBS.
• Synonyms: HLA-A, Antibody; HLA-A Antibody (Center); HLA class I histocompatibility antigen; A-3 alpha chain; MHC class I antigen A*3; HLA-A; HLAA; anti-HLA-A antibody

• Host: Mouse
• Reactivity: Mouse (Predicted Reactivity: Human)
• Clonality: Monoclonal
• Isotype: IgG1,Ig
• Specificity: This HLA-A antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 70-99 amino acids from the Central region of human HLA-A.
• Purity/Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.
• Form/Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Positions: 70-99
• Sequence Length: 365

• Applicable Applications for anti-HLA-A antibody: ELISA, WB (Western Blot)
• Function: Antigen-presenting major histocompatibility complex class I (MHCI) molecule. In complex with B2M/beta 2 microglobulin displays primarily viral and tumor-derived peptides on antigen-presenting cells for recognition by alpha-beta T cell receptor (TCR) on HLA-A-restricted CD8-positive T cells, guiding antigen-specific T cell immune response to eliminate infected or transformed cells (PubMed:2456340, PubMed:2784196, PubMed:1402688, PubMed:7504010, PubMed:9862734, PubMed:10449296, PubMed:12138174, PubMed:12393434, PubMed:15893615, PubMed:17189421, PubMed:19543285, PubMed:21498667, PubMed:24192765, PubMed:7694806, PubMed:24395804, PubMed:28250417). May also present self-peptides derived from the signal sequence of secreted or membrane proteins, although T cells specific for these peptides are usually inactivated to prevent autoreactivity (PubMed:25880248, PubMed:7506728, PubMed:7679507). Both the peptide and the MHC molecule are recognized by TCR, the peptide is responsible for the fine specificity of antigen recognition and MHC residues account for the MHC restriction of T cells (PubMed:12796775, PubMed:18275829, PubMed:19542454, PubMed:28250417). Typically presents intracellular peptide antigens of 8 to 13 amino acids that arise from cytosolic proteolysis via IFNG-induced immunoproteasome or via endopeptidase IDE/insulin-degrading enzyme (PubMed:17189421, PubMed:20364150, PubMed:17079320, PubMed:26929325, PubMed:27049119). Can bind different peptides containing allele- specific binding motifs, which are mainly defined by anchor residues at position 2 and 9 (PubMed:7504010, PubMed:9862734).
• Cellular Location: Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein
• Tissue Location: Ubiquitous..
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(HLA-A Antibody western blot analysis in mouse Liver tissue lysates (35ug/lane).This demonstrates the HLA-A antibody detected the HLA-A protein (arrow).)

Related Product Information for anti-HLA-A antibody

HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described.

Product Categories/Family for anti-HLA-A antibody

Immunology

References

HLA Class I and Genetic Susceptibility to Type 1 Diabetes: Results from the Type 1 Diabetes Genetics Consortium. Noble JA, et al. Diabetes, 2010 Aug 26. PMID 20798335. Effect of HLA Class I and Class II Alleles on Progression from Autoantibody Positivity to Overt Type 1 Diabetes in Children with Risk-Associated Class II Genotypes. Lipponen K, et al. Diabetes, 2010 Aug 25. PMID 20739684. Infectious mononucleosis-linked HLA class I single nucleotide polymorphism is associated with multiple sclerosis. Jafari N, et al. Mult Scler, 2010 Aug 24. PMID 20736246. [Associations of Human Leukocyte Antigen-A, B, DRB1 Genes with Leukemia Patients in Anhui Province of China.] Liao YQ, et al. Zhongguo Shi Yan Xue Ye Xue Za Zhi, 2010 Jul. PMID 20723328. Longer survival associated with HLA-A*03, B*14 among 212 hemochromatosis probands with HFE C282Y homozygosity and HLA-A and -B typing and haplotyping. Barton JC, et al. Eur J Haematol, 2010 Aug 14. PMID 20722701.

NCBI and Uniprot Product Information

• NCBI GI #: 24797067
• NCBI GeneID: 3105
• NCBI Accession #: NP_002107.3
• NCBI GenBank Nucleotide #: NM_002116.7
• UniProt Accession #: P04439; O19546; O19756; Q65A82; Q9GJE6; Q9GJE7; Q9GJE8; Q9MYE6; Q9MYG4; Q9TPR8; P01891; P01892; P05534; P10314; P10316; P13746; P16189; P18462; P30443
• Molecular Weight: Calculated MW: 40841
• NCBI Official Full Name: HLA class I histocompatibility antigen, A-1 alpha chain
• NCBI Official Synonym Full Names: major histocompatibility complex, class I, A
• NCBI Official Symbol: HLA-A
• NCBI Official Synonym Symbols: HLAA
• NCBI Protein Information: HLA class I histocompatibility antigen, A-1 alpha chain
• UniProt Protein Name: HLA class I histocompatibility antigen, A-3 alpha chain
• UniProt Gene Name: HLA-A
• UniProt Synonym Gene Names: HLAA
• UniProt Entry Name: 1A03_HUMAN