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Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human HLA-A2: RPE)
Mouse HLA A2 Monoclonal Antibody | anti-HLA A2 antibody
MOUSE ANTI HUMAN HLA A2
Gene Names
HLA-A; HLAA
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation
Synonyms
HLA A2, Antibody; MOUSE ANTI HUMAN HLA A2; anti-HLA A2 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
BB7.2
Form/Format
Purified
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Sequence Length
365
Applicable Applications for anti-HLA A2 antibody
Immunohistology Frozen, Flow cytometry (FC/FACS), Immunoprecipitation (IP)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
Flow Cytometry: Maximum Dilution: 10ug/ml
Flow Cytometry: Maximum Dilution: 10ug/ml
Perservative Stabilisers
0.09% Sodium Azide
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Specificity
HLA A2
Histology Positive Control Tissue
Tonsil
Buffer Solution
Phosphate buffered saline
Target Species
Human
Immunogen
Papain solubilised HLA-A2
Fusion Partner
Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Preparation and Storage
Store at 4°C or at -20°C if preferred. This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human HLA-A2: RPE)
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human HLA-A2: RPE)
Application Data
(Staining of human peripheral blood monocytes with Mouse anti Human HLA-A2:FITC)
Application Data
(Staining of human peripheral blood monocytes with Mouse anti Human HLA-A2:FITC)
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human HLA-A2: endotoxin low)
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human HLA-A2: endotoxin low)
Application Data
(Staining of human peripheral blood monocytes with Mouse anti Human HLA-A2:Alexa Fluor 488 (AAA11950A488))
Application Data
(Staining of human peripheral blood monocytes with Mouse anti Human HLA-A2:Alexa Fluor 488 (AAA11950A488))
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human HLA-A2)
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human HLA-A2)
Related Product Information for anti-HLA A2 antibody
Mouse anti Human HLA A2 antibody, clone BB7.2 recognizes the human HLA-A2 histocompatability antigen. The epitope recognized by this antibody has been studied extensively and would appear to include the carboxy-terminus of the alpha-2 helix and a turn on one of the underlying beta strands. Mouse anti Human HLA A2 antibody, clone BB7.2 may be used for the flow cytometric detection of HLA-A2 expression and has also been used for immunoaffinity purification of HLA-A2 molecules. Functionally Mouse anti Human HLA A2 antibody, clone BB7.2 is reported to inhibit MHC restricted cellular cytotoxicity..
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
HLA class I histocompatibility antigen, A-2 alpha chain isoform X2
NCBI Official Synonym Full Names
major histocompatibility complex, class I, A
NCBI Official Symbol
HLA-A
NCBI Official Synonym Symbols
HLAA
NCBI Protein Information
HLA class I histocompatibility antigen, A-1 alpha chain; MHC class I antigen HLA-A heavy chain; antigen presenting molecule; leukocyte antigen class I-A
UniProt Protein Name
HLA class I histocompatibility antigen, A-2 alpha chain
UniProt Gene Name
HLA-A
UniProt Synonym Gene Names
HLAA
UniProt Entry Name
1A02_HUMAN
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Product Notes
The HLA A2 hla-a (Catalog #AAA11950) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HLA A2 can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, Flow cytometry (FC/FACS), Immunoprecipitation (IP). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood. Flow Cytometry: Maximum Dilution: 10ug/ml. Researchers should empirically determine the suitability of the HLA A2 hla-a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HLA A2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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