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Mouse anti-Human HLA-DR Monoclonal Antibody | anti-HLA-DR antibody
Mouse anti-HLA-DR Monoclonal Antibody, Purified [LN3]
Gene Names
HLA-DRA; MLRW; HLA-DRA1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Purified
Synonyms
HLA-DR, Antibody; Mouse anti-HLA-DR Monoclonal Antibody, Purified [LN3]; MHC class II antigen DRA; HLA-DRA1; histocompatibility antigen HLA-DR alpha; HLA class II histocompatibility antigen, DR alpha chain; major histocompatibility complex, class II, DR alpha; anti-HLA-DR antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Clone Number
LN3
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Phosphate Buffered Saline (PBS) with 0.1% BSA and 0.09% Sodium Azide
Concentration
50ug/ml (varies by lot)
Applicable Applications for anti-HLA-DR antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry-Immunofluorescence (IHC-IF), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000
IHC: 1:100-1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
ICC: 1:100-1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.
IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
FC/FACS: Live or cells fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.5 ul per 1 x 10^6 cells.
IHC: 1:100-1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
ICC: 1:100-1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.
IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
FC/FACS: Live or cells fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.5 ul per 1 x 10^6 cells.
Immunogen
Activated human peripheral blood mononuclear cells
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human HLA-DR by western blot. Samples: Whole cell lysate (50 ug) from KG-1, SNB-75, Raji, Ramos, and SR (10 ug) cells prepared using NETN lysis buffer. Antibody: Mouse anti-HLA-DR monoclonal antibody [LN3] (AAA23780 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
IHC (Immunohistochemistry)
(Detection of human HLA-DR (red) by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Mouse anti-HLA-DR monoclonal antibody [LN3] (AAA23780 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-Mouse IgG . Substrate: Opal. Counterstain: DAPI (blue).)
IHC (Immunohistochemistry)
(Detection of human HLA-DR by immunhistochemistry. Sample: FFPE section of human tonsil. Antibody: Mouse monoclonal anti-HLA-DR antibody [LN3] (AAA23780 lot 1) used at 1:100. Secondary: DyLight 594-conjugated goat anti-mouse IgG .)
IHC (Immunohistochemistry)
(Detection of human HLA-DR by immunohistochemistry. Sample: FFPE section of tonsil. Antibody: Mouse anti-HLA-DR monoclonal antibody [LN3] (AAA23780-2). Secondary: HRP-conjugated goat anti-mouse IgG .)
ICC (Immunocytochemistry)
(Detection of human HLA-DR by immunocytochemistry. Sample: FFPE section of SR cells. Antibody: Mouse anti-HLA-DR monoclonal antibody [LN3] (AAA23780-2). Secondary: HRP-conjugated goat anti-mouse IgG .)
FCM (Flow Cytometry)
(Detection of human HLA-DR (shaded) in Daudi cells by flow cytometry. Antibody: Mouse anti-HLA-DR monoclonal antibody [LN3] (AAA23780) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-mouse IgG .)
Related Product Information for anti-HLA-DR antibody
HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5 [taken from NCBI Entrez Gene (Gene ID: 3122)].
Product Categories/Family for anti-HLA-DR antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,607 Da
NCBI Official Full Name
HLA class II histocompatibility antigen, DR alpha chain
NCBI Official Synonym Full Names
major histocompatibility complex, class II, DR alpha
NCBI Official Symbol
HLA-DRA
NCBI Official Synonym Symbols
MLRW; HLA-DRA1
NCBI Protein Information
HLA class II histocompatibility antigen, DR alpha chain; MHC cell surface glycoprotein; MHC class II antigen DRA; histocompatibility antigen HLA-DR alpha
UniProt Protein Name
HLA class II histocompatibility antigen, DR alpha chain
UniProt Gene Name
HLA-DRA
UniProt Synonym Gene Names
HLA-DRA1
UniProt Entry Name
DRA_HUMAN
