IF (Immunofluorescence)
(ImmunofluorescencestainingofDaudicellswithanti-HLA-DRantibodyCAT13.3H3(AAA72511). ImmunofluorescenceanalysisofparaformaldehydefixedDaudicellsonShi-fixcoverslipsstainedwiththechimericrabbitIgGversionofCAT13.3H3(AAA72511)(1:100dilution)for1hfollowedbyAlexaFluor488secondaryantibody(1:1000dilution),showingmembraneandcytoplasmicstaining.ThenuclearstainisDAPI(blue).Panelsshow,fromleft-right,top-bottom,AAA72511,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
HLA-DR recombinant antibody
Anti-HLA-DR [CAT 13.3H3], Rabbit IgG, kappa
Gene Names
CD74; II; DHLAG; HLADG; Ia-GAMMA
Reactivity
Human
Purity
Protein A affinity purified
Synonyms
HLA-DR, Recombinant Antibody; Anti-HLA-DR [CAT 13.3H3], Rabbit IgG, kappa; HLA-DR; Anti-HLA-DR [CAT 13.3H3]; CD74; HLA class II histocompatibility antigen gamma chain; HLA-DR antigens-associated invariant chain; Ia antigen-associated invariant chain; Ii; p33; CAT 13.8H7; CAT13.8H7; 13.8H7; HLA-DR recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG kappa
Clone Number
CAT 13.3H3
Specificity
The antibody CAT 13.3H3 recognizes and is directed against HLA-DR.
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
IF (Immunofluorescence)
(ImmunofluorescencestainingofDaudicellswithanti-HLA-DRantibodyCAT13.3H3(AAA72511). ImmunofluorescenceanalysisofparaformaldehydefixedDaudicellsonShi-fixcoverslipsstainedwiththechimericrabbitIgGversionofCAT13.3H3(AAA72511)(1:100dilution)for1hfollowedbyAlexaFluor488secondaryantibody(1:1000dilution),showingmembraneandcytoplasmicstaining.ThenuclearstainisDAPI(blue).Panelsshow,fromleft-right,top-bottom,AAA72511,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
IF (Immunofluorescence)
(ImmunofluorescencestainingofDaudicellswithanti-HLA-DRantibodyCAT13.3H3(AAA72511). ImmunofluorescenceanalysisofparaformaldehydefixedDaudicellsonShi-fixcoverslipsstainedwiththechimericrabbitIgGversionofCAT13.3H3(AAA72511)(1:100dilution)for1hfollowedbyAlexaFluor488secondaryantibody(1:1000dilution),showingmembraneandcytoplasmicstaining.ThenuclearstainisDAPI(blue).Panelsshow,fromleft-right,top-bottom,AAA72511,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
FCM/FACS (Flow Cytometry)
(Flowcytometryusingtheanti-HLA-DRantibodyCAT13.3H3(AAA72511). Daudicellswerestainedwiththeanti-unknownspecificityantibodyortherabbitIgGversionofCAT13.3H3(AAA72511,blueline)atadilutionof1:100for1hatRT.Afterwashing,theboundantibodywasdetectedusingagoatanti-rabbitIgGAlexaFluor488antibodyatadilutionof1:1000andthecellswereanalyzedusingaFACSCantoflow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flowcytometryusingtheanti-HLA-DRantibodyCAT13.3H3(AAA72511). Daudicellswerestainedwiththeanti-unknownspecificityantibodyortherabbitIgGversionofCAT13.3H3(AAA72511,blueline)atadilutionof1:100for1hatRT.Afterwashing,theboundantibodywasdetectedusingagoatanti-rabbitIgGAlexaFluor488antibodyatadilutionof1:1000andthecellswereanalyzedusingaFACSCantoflow-cytometer.)
Related Product Information for HLA-DR recombinant antibody
This antibody was raised by immunizing mice against HLA-DR.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
296
NCBI Official Full Name
HLA class II histocompatibility antigen gamma chain
NCBI Official Synonym Full Names
CD74 molecule, major histocompatibility complex, class II invariant chain
NCBI Official Symbol
CD74
NCBI Official Synonym Symbols
II; DHLAG; HLADG; Ia-GAMMA
NCBI Protein Information
HLA class II histocompatibility antigen gamma chain; p33; HLA-DR-gamma; MHC HLA-DR gamma chain; Ia-associated invariant chain; gamma chain of class II antigens; HLA-DR antigens-associated invariant chain; CD74 antigen (invariant polypeptide of major histo
UniProt Protein Name
HLA class II histocompatibility antigen gamma chain
UniProt Gene Name
CD74
UniProt Synonym Gene Names
DHLAG; Ii
UniProt Entry Name
HG2A_HUMAN
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Product Notes
The HLA-DR cd74 (Catalog #AAA72511) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-HLA-DR [CAT 13.3H3], Rabbit IgG, kappa reacts with Human and may cross-react with other species as described in the data sheet. It is sometimes possible for the material contained within the vial of "HLA-DR, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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