WB (Western Blot)
(Detection of mouse hnRNP M3/4 by western blot. Samples: Whole cell lysate (10 ug) from BW5147.3, CT26, CH27, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Mouse anti-hnRNP M3/4 monoclonal antibody [2A6-2H3] (AAA213497 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
Mouse anti-Human, Mouse hnRNP M3/4 Monoclonal Antibody | anti-hnRNPM3/4 antibody
Mouse anti-hnRNP M3/4 Monoclonal Antibody, Purified [2A6-2H3]
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of mouse hnRNP M3/4 by western blot. Samples: Whole cell lysate (10 ug) from BW5147.3, CT26, CH27, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Mouse anti-hnRNP M3/4 monoclonal antibody [2A6-2H3] (AAA213497 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot)
(Detection of mouse hnRNP M3/4 by western blot. Samples: Whole cell lysate (10 ug) from BW5147.3, CT26, CH27, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Mouse anti-hnRNP M3/4 monoclonal antibody [2A6-2H3] (AAA213497 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot)
(Detection of human hnRNP M3/4 by western blot. Samples: Whole cell lysate (5 ug) from HEK293T, LNCaP, A-549, MCF-7, and K-562 cells prepared using NETN lysis buffer. Antibody: Mouse anti-hnRNP M3/4 monoclonal antibody [2A6-2H3] (AAA213497 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG . Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot)
(Detection of human hnRNP M3/4 by western blot. Samples: Whole cell lysate (5 ug) from HEK293T, LNCaP, A-549, MCF-7, and K-562 cells prepared using NETN lysis buffer. Antibody: Mouse anti-hnRNP M3/4 monoclonal antibody [2A6-2H3] (AAA213497 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG . Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
IP (Immunoprecipitation)
(Detection of human hnRNP M3/4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg per IP; 10% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Mouse anti-hnRNP M3/4 monoclonal antibody [2A6-2H3] (AAA213497 lot 3) used for IP at 20 ul/mg lysate. hnRNP M3/4 was also immunoprecipitated by a previous lot of this antibody (AAA213497 lot 2) and a second antibody against a different epitope of hnRNP M3/4 . For blotting immunoprecipitated hnRNP M3/4, AAA213497 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.)
IP (Immunoprecipitation)
(Detection of human hnRNP M3/4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg per IP; 10% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Mouse anti-hnRNP M3/4 monoclonal antibody [2A6-2H3] (AAA213497 lot 3) used for IP at 20 ul/mg lysate. hnRNP M3/4 was also immunoprecipitated by a previous lot of this antibody (AAA213497 lot 2) and a second antibody against a different epitope of hnRNP M3/4 . For blotting immunoprecipitated hnRNP M3/4, AAA213497 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.)
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Product Notes
The hnRNPM3/4 hnrnpm (Catalog #AAA213497) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti-hnRNP M3/4 Monoclonal Antibody, Purified [2A6-2H3] reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's hnRNP M3/4 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the hnRNPM3/4 hnrnpm for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "hnRNP M3/4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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