IF (Immunofluorescence)
(ICC/IF analysis of HPRT in HaLe cells. The cell was stained with AAA48956 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
Mouse anti-Human HPRT Monoclonal Antibody | anti-HPRT antibody
HPRT antibody
Gene Names
HPRT1; HPRT; HGPRT
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Flow Cytometry, Functional Assay, Western Blot, ELISA
Purity
By protein-A affinity chromatography
Synonyms
HPRT, Antibody; HPRT antibody; Hypoxanthine-guanine phosphoribosyltransferase; HGPRT; HGPRTase; HPRT; Hypoxanthine-guanine phosphoribosyltransferase HPRT 1; HPRT1; Hypoxanthine guanine phosphoribosyltransferase; Hypoxanthine phosphoribosyltransferase 1 (Lesch Nyhan syndrome); Hypoxanthine phosphoribosyltransferase 1.; anti-HPRT antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
AT1D9
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
218
Applicable Applications for anti-HPRT antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA
Immunogen
Recombinant human HPRT (1-218aa) purified from E. coli.
Preparation and Storage
Can be stored at +2°C to +8°C short term. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
IF (Immunofluorescence)
(ICC/IF analysis of HPRT in HaLe cells. The cell was stained with AAA48956 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
IF (Immunofluorescence)
(ICC/IF analysis of HPRT in HaLe cells. The cell was stained with AAA48956 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
WB (Western Blot)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HPRT antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: MCF7 cell lysate Lane 3.: 293T cell lysate Lane 4.: A549 cell lysate)
WB (Western Blot)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HPRT antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: MCF7 cell lysate Lane 3.: 293T cell lysate Lane 4.: A549 cell lysate)
Related Product Information for anti-HPRT antibody
HPRT is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. HPRT, which acts as a catalyst in the reaction between guanine and phosphoribosyl pyrophosphate to form GMP, functions primarily to salvage purines from degraded DNA to renewed purine synthesis.
Product Categories/Family for anti-HPRT antibody
References
Sculley DG, et al. (1993). Hum. Genet. 90 (3): 195-207.; Stout JT, Caskey CT (1986). Annu. Rev. Genet. 19: 127-48.; Davidson BL, et al. (1991). Am. J. Hum. Genet. 48 (5): 951-8.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
hypoxanthine-guanine phosphoribosyltransferase
NCBI Official Synonym Full Names
hypoxanthine phosphoribosyltransferase 1
NCBI Official Symbol
HPRT1
NCBI Official Synonym Symbols
HPRT; HGPRT
NCBI Protein Information
hypoxanthine-guanine phosphoribosyltransferase
UniProt Protein Name
Hypoxanthine-guanine phosphoribosyltransferase
UniProt Gene Name
HPRT1
UniProt Synonym Gene Names
HPRT; HGPRT; HGPRTase
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Product Notes
The HPRT hprt1 (Catalog #AAA48956) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The HPRT antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HPRT can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the HPRT hprt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HPRT, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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