Mouse HSP70/HSC70 Monoclonal Antibody | anti-HSP70/HSC70 antibody

HSP70/HSC70 Antibody

Cat. Num. AAA253927

• Gene Names: HSPA1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70-2; HSP70.1; HSP70.2; HSP70-1A; HEL-S-103
• Reactivity: Human, Mouse, Rat, Bovine, Caenorhabditis elegans, Beluga, Dog, Chicken, Fruit Fly, Fish, Guinea Pig, Hamster, Monkey, Pig, Plant, Cucumber, Pea, Rabbit, Sheep, African Clawed Frog
• Applications: Flow Cytometry, Functional Assay, Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
• Purity: Protein G Purified
• Synonyms: HSP70/HSC70, Antibody; HSP70/HSC70 Antibody; Mouse Anti-Human HSP70/HSC70 Monoclonal IgG1; HSP70/HSC70 Antibody, Clone N27F3-4; HSP70 1 Antibody; HSP70 2 Antibody; HSP70.1 Antibody; HSP72 Antibody; HSPA1 Antibody; HSPA1A Antibody; HSPA1B Antibody; anti-HSP70/HSC70 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat, Bovine, Caenorhabditis elegans, Beluga, Dog, Chicken, Fruit Fly, Fish, Guinea Pig, Hamster, Monkey, Pig, Plant, Cucumber, Pea, Rabbit, Sheep, African Clawed Frog
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: N27F3-4
• Specificity: Detects ~72 (HSP) and ~73kDa (HSC).
• Purity/Purification: Protein G Purified
• Form/Format: PBS pH 7.2, 50% glycerol, 0.09% Sodium Azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 641

• Applicable Applications for anti-HSP70/HSC70 antibody: IEM (Immuno-ElectronMicroscopy), FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: Recombinant HSP70/HSC70
• Immunogen Species: Human
• Conjugate: Unconjugated
• Cellular Localization: Cytoplasm
• Certificate of Analysis: 1 ug/ml of SMC-104 was sufficient for detection of HSP70/HSC70 in 20 ug of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Preparation and Storage: Store at-20 degree C.

WB (Western Blot)

(Western Blot analysis of Human Cell lysates showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone N27 . Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)

IHC (Immunohistochemistry)

(Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone N27 . Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.)

Related Product Information for anti-HSP70/HSC70 antibody

HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50% identity (2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All HSP70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the HSP70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of HSP70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.

Product Categories/Family for anti-HSP70/HSC70 antibody

Cell Signaling; Protein Trafficking; Chaperone Proteins; Cancer; Tumor Biomarkers; Cancer; Heat Shock

References

1. Welch W.J. and Suhan J.P. (1986) J.Cell Biol. 103: 2035-2050. 2. Boorstein W. R., Ziegelhoffer T. & Craig E. A. (1993), J. Mol. Evol.38 (1): 1-17. 3. Rothman J. (1989) Cell 59: 591-601. 4. DeLuca-Flaherty et al. (1990), Cell 62: 875-887. 5. Bork P., Sander C. & Valencia A. (1992) Proc. Nut1 Acad. Sci. USA 89: 7290-7294. 6. Fink A.L. (1999) Physiol. Rev. 79: 425-449. 7. Polanonka-Grabowska R. et. al. (1997) Blood 90: 1516-1526. 8. Schnell D.J. et. al. (1994) Science 266: 1007-1012. 9. Kabakov A.E., et. al. (2002) Am. J.Physiol. 283(2): C521-C534. 10. Ricart J. et. al. (1997) Biochem. J. 324: 635-643. 11. Hang H. and Fox M.H. (1995) Cytometry 19(2): 119-125.

NCBI and Uniprot Product Information

• NCBI GI #: 194248072
• NCBI GeneID: 3303
• NCBI Accession #: NP_005336.3
• NCBI GenBank Nucleotide #: NM_005345.5
• UniProt Accession #: P0DMV8; P0DMV9
• NCBI Official Full Name: heat shock 70 kDa protein 1A
• NCBI Official Synonym Full Names: heat shock protein family A (Hsp70) member 1A
• NCBI Official Symbol: HSPA1A
• NCBI Official Synonym Symbols: HSP72; HSPA1; HSP70I; HSP70-1; HSP70-2; HSP70.1; HSP70.2; HSP70-1A; HEL-S-103
• NCBI Protein Information: heat shock 70 kDa protein 1A
• UniProt Protein Name: Heat shock 70 kDa protein 1A
• UniProt Gene Name: HSPA1A
• UniProt Synonym Gene Names: HSP721 PublicationManual assertion based on opinion iniRef.41; HSP70-12 PublicationsManual assertion based on opinion iniRef.4; HSP70.1