IHC (Immunohiostchemistry)
(IHC image diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
HTR2C recombinant antibody
HTR2C Antibody
Gene Names
HTR2C; HTR1C; 5-HT2C; 5-HTR2C
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
Affinity-chromatography
Synonyms
HTR2C, Recombinant Antibody; HTR2C Antibody; 5-hydroxytryptamine receptor 2C (5-HT-2C) (5-HT2C) (5-HTR2C) (5-hydroxytryptamine receptor 1C) (5-HT-1C) (5-HT1C) (Serotonin receptor 2C); HTR2C; HTR1C; HTR2C recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
9H8
Purity/Purification
Affinity-chromatography
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, PH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applicable Applications for HTR2C recombinant antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA
Antibody Type
Recombinant Antibody
Conjugation
Non-conjugated
Immunogen
A synthesized peptide derived from human 5HT2C Receptor
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
IHC (Immunohiostchemistry)
(IHC image diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
IHC (Immunohiostchemistry)
(IHC image diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
WB (Western Blot)
(Western BlotPositive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysateAll lanes: HTR2C antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 52, 29 kDaObserved band size: 75 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysateAll lanes: HTR2C antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 52, 29 kDaObserved band size: 75 kDa)
Related Product Information for HTR2C recombinant antibody
G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances, including ergot alkaloid derivatives, 1-2,5,-dimethoxy-4-iodophenyl-2-aminopropane (DOI) and lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and down-stream signaling cascades and promotes the release of Ca(2+) ions from intracellular stores. Regulates neuronal activity via the activation of short transient receptor potential calcium channels in the brain, and thereby modulates the activation of pro-opiomelacortin neurons and the release of CRH that then regulates the release of corticosterone. Plays a role in the regulation of appetite and eating behavior, responses to anxiogenic stimuli and stress. Plays a role in insulin sensitivity and glucose homeostasis.
Product Categories/Family for HTR2C recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
51,821 Da
NCBI Official Full Name
5-hydroxytryptamine receptor 2C isoform a
NCBI Official Synonym Full Names
5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled
NCBI Official Symbol
HTR2C
NCBI Official Synonym Symbols
HTR1C; 5-HT2C; 5-HTR2C
NCBI Protein Information
5-hydroxytryptamine receptor 2C; 5-HT1C; 5-HT-1C; 5-HT-2C; serotonin receptor 2C; serotonin 5-HT-2C receptor; 5-hydroxytryptamine receptor 1C; 5-hydroxytryptamine receptor-1c
UniProt Protein Name
5-hydroxytryptamine receptor 2C
UniProt Gene Name
HTR2C
UniProt Synonym Gene Names
HTR1C; 5-HT-2C; 5-HT2C; 5-HTR2C; 5-HT-1C; 5-HT1C
UniProt Entry Name
5HT2C_HUMAN
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Product Notes
The HTR2C htr2c (Catalog #AAA243935) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HTR2C Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HTR2C can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the HTR2C htr2c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HTR2C, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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