IHC (Immunohistochemistry)
(IgG positive plasma cells in the case of IgG4-related chronic sclerosing sialoadenitis, detected with anti-IgG antibody. Formalin fixed, paraffin embedded human tissue (4 um section).)
Rabbit Human IgG Monoclonal Antibody | anti-IGHG1 antibody
Anti - Human IgG
Reactivity
Human
Applications
Immunohistochemistry
Synonyms
Human IgG, Antibody; Anti - Human IgG; anti-IGHG1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
I17-T
Sequence Length
330
Applicable Applications for anti-IGHG1 antibody
IHC (Immunohistochemistry)
Immunogen
Peptide derived from the domain close to the C-terminus of human IgG-1 chain C region. Antibody recognizes the epitope between Ala261 - Lys275
Storage Buffer
20 mM Tris-HCl, pH 8.0
Stabilizer
20 mg/ml BSA
Preservative
0.05% NaN3
Cellular Location
Secreted
Postive Control
Human tonsil tissue
IHC-P Protocol
1. Deparaffinize the section in 3 changes of xylene, 10 minutes each.
2. Wash the section in 96%, 80% and 70% ethyl alcohol for 10 minutes each.
3. Rinse in distilled water, 2 x 5 minutes.
4. Block the endogenous peroxidase by incubating the tissue in 3% hydrogen peroxide (H2O2) for 10 minutes.
5. Wash in distilled water, 2 x 5 minutes.
6. For antigen retrieval: Immerse the slide in Tris-EDTA buffer, pH 9.0 and incubate at 95-97ºC in water bath for 25 minutes.
7. Remove the staining to room temperature and let the slide to cool (in Tris-EDTA buffer, pH 9.0) for 15 minutes.
8. Rinse in distilled water, 2 x 5 minutes. .
9. Wash in PBS (phosphate buffer saline, pH 7.0-7.5) supplemented with 0.05% of Tween-20 (buffer A), 2 x 5 minutes.
10. CONCENTRATED:
Incubate the section with primary antibody at the dilution 1:100 - 1:300 for 1 hour in the closed wet chamber.
READY TO USE (RTU):
Incubate the section with primary antibody (ready to use) for 1 hour in a closed wet chamber.
11. Wash 3 x 5 minutes with buffer A.
12. Apply the secondary antibody (the protocol depends on the supplier), and proceed to standard immunohistochemistry protocol (HRP - Peroxide - DAB). Micropolymer-HRP detection kit rabbit/mouse dual is suggested. (Please inquire)
13. Wash 3 x 5 minutes with buffer A.
14. Apply the chromogen (DAB), 1 - 3 minutes.
15. Wash in water, 2 x 5 minutes.
16. Stain in hematoxylin for 5 minutes.
17. Wash in distilled water, 3 x 2 minutes.
18. Mount the slide for observation.
* Tris-EDTA Buffer (10mM Tris Base, 1mM EDTA solution, 0.05% Tween-20, pH 9.0):
Tris ----------- 1.21 g; EDTA ---------- 0.37 g; Distilled water ------------ 1000 ml .
Mix to dissolve in 700 ml of distilled water. Adjust pH to 9.0 with 1M HCl and then add.
0.5 ml of Tween-20 and mix well. Adjust the final volume to 1 liter with distilled water.
Store this solution at room temperature for 3 months or at +4ºC for longer storage.
2. Wash the section in 96%, 80% and 70% ethyl alcohol for 10 minutes each.
3. Rinse in distilled water, 2 x 5 minutes.
4. Block the endogenous peroxidase by incubating the tissue in 3% hydrogen peroxide (H2O2) for 10 minutes.
5. Wash in distilled water, 2 x 5 minutes.
6. For antigen retrieval: Immerse the slide in Tris-EDTA buffer, pH 9.0 and incubate at 95-97ºC in water bath for 25 minutes.
7. Remove the staining to room temperature and let the slide to cool (in Tris-EDTA buffer, pH 9.0) for 15 minutes.
8. Rinse in distilled water, 2 x 5 minutes. .
9. Wash in PBS (phosphate buffer saline, pH 7.0-7.5) supplemented with 0.05% of Tween-20 (buffer A), 2 x 5 minutes.
10. CONCENTRATED:
Incubate the section with primary antibody at the dilution 1:100 - 1:300 for 1 hour in the closed wet chamber.
READY TO USE (RTU):
Incubate the section with primary antibody (ready to use) for 1 hour in a closed wet chamber.
11. Wash 3 x 5 minutes with buffer A.
12. Apply the secondary antibody (the protocol depends on the supplier), and proceed to standard immunohistochemistry protocol (HRP - Peroxide - DAB). Micropolymer-HRP detection kit rabbit/mouse dual is suggested. (Please inquire)
13. Wash 3 x 5 minutes with buffer A.
14. Apply the chromogen (DAB), 1 - 3 minutes.
15. Wash in water, 2 x 5 minutes.
16. Stain in hematoxylin for 5 minutes.
17. Wash in distilled water, 3 x 2 minutes.
18. Mount the slide for observation.
* Tris-EDTA Buffer (10mM Tris Base, 1mM EDTA solution, 0.05% Tween-20, pH 9.0):
Tris ----------- 1.21 g; EDTA ---------- 0.37 g; Distilled water ------------ 1000 ml .
Mix to dissolve in 700 ml of distilled water. Adjust pH to 9.0 with 1M HCl and then add.
0.5 ml of Tween-20 and mix well. Adjust the final volume to 1 liter with distilled water.
Store this solution at room temperature for 3 months or at +4ºC for longer storage.
Preparation and Storage
Store at +4°C.
IHC (Immunohistochemistry)
(IgG positive plasma cells in the case of IgG4-related chronic sclerosing sialoadenitis, detected with anti-IgG antibody. Formalin fixed, paraffin embedded human tissue (4 um section).)
IHC (Immunohistochemistry)
(IgG positive plasma cells in the case of IgG4-related chronic sclerosing sialoadenitis, detected with anti-IgG antibody. Formalin fixed, paraffin embedded human tissue (4 um section).)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
36,106 Da
NCBI Official Full Name
Ig gamma-1 chain C region
NCBI Official Synonym Full Names
immunoglobulin heavy constant gamma 1 (G1m marker)
NCBI Official Symbol
IGHG1
UniProt Protein Name
Ig gamma-1 chain C region
UniProt Gene Name
IGHG1
UniProt Entry Name
IGHG1_HUMAN
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Product Notes
The IGHG1 ighg1 (Catalog #AAA79014) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti - Human IgG reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Human IgG can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the IGHG1 ighg1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Human IgG, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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