WB (Western Blot)
(Various lysates were subjected to SDS PAGE followed by western blot with Hypusine antibody at 1ug/ml.Lane 1: Hela cell lysateLane 2: HepG2 cell lysateLane 3: K562 cell lysateLane 4: PC-12 cell lysateLane 5: Jurkat cell lysateLane 6: MCF-7 cell lysateLane 7: 293T cell lysateSecond Ab: Goat Anti-Rabit IgG H&L Polyclonal antibody, HRP (Please Inquire) at 0.1ug/ml.Predict MW: 17 kDaObserve MW: 17 kDa)
Hypusine Recombinant Antibody
Anti-Hypusine antibody (Hpu24)
Reactivity
General
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
>95% by SDS-PAGE. Protein A or G purified from cell culture supernatant.
Synonyms
Hypusine, Recombinant Antibody; Anti-Hypusine antibody (Hpu24); Hypusine; Eukaryotic translation initiation factor 5A-1; eIF-5A-1; eIF-5A1; Eukaryotic initiation factor 5A isoform 1; eIF-5A; Rev-binding factor; eIF-4D; EIF5A; Hypusine recombinant antibody
Host
Rabbit
Reactivity
General
Clonality
Monoclonal
Isotype
IgG
Clone Number
Hpu24
Purity/Purification
>95% by SDS-PAGE. Protein A or G purified from cell culture supernatant.
Form/Format
Liquid; 0.01M PBS, pH 7.4.
Concentration
1mg/ml (varies by lot)
Applicable Applications for Hypusine recombinant antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
PubChem
65396
Expression System
Mammalian Cells
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at -20 degree C for short term. Store at -80 degree C long term.
WB (Western Blot)
(Various lysates were subjected to SDS PAGE followed by western blot with Hypusine antibody at 1ug/ml.Lane 1: Hela cell lysateLane 2: HepG2 cell lysateLane 3: K562 cell lysateLane 4: PC-12 cell lysateLane 5: Jurkat cell lysateLane 6: MCF-7 cell lysateLane 7: 293T cell lysateSecond Ab: Goat Anti-Rabit IgG H&L Polyclonal antibody, HRP (Please Inquire) at 0.1ug/ml.Predict MW: 17 kDaObserve MW: 17 kDa)
WB (Western Blot)
(Various lysates were subjected to SDS PAGE followed by western blot with Hypusine antibody at 1ug/ml.Lane 1: Hela cell lysateLane 2: HepG2 cell lysateLane 3: K562 cell lysateLane 4: PC-12 cell lysateLane 5: Jurkat cell lysateLane 6: MCF-7 cell lysateLane 7: 293T cell lysateSecond Ab: Goat Anti-Rabit IgG H&L Polyclonal antibody, HRP (Please Inquire) at 0.1ug/ml.Predict MW: 17 kDaObserve MW: 17 kDa)
References
Characterization of a novel polyclonal anti-hypusine antibody. PMID: 24024105
Synthesis of reagents for the construction of hypusine and deoxyhypusine peptides and their application as peptidic antigens. PMID: 9748364
Translation initiation factor eIF-5A, the hypusine-containing protein, is phosphorylated on serine and tyrosine and O-glycosylated in Trichomonas vaginalis. PMID: 22212642
Polyamines and their derivatives as modulators in growth and differentiation. PMID: 2697982
Differential expression of genes encoding the hypusine-containing translation initiation factor, eIF-5A, in tobacco. PMID: 1542563
Synthesis of reagents for the construction of hypusine and deoxyhypusine peptides and their application as peptidic antigens. PMID: 9748364
Translation initiation factor eIF-5A, the hypusine-containing protein, is phosphorylated on serine and tyrosine and O-glycosylated in Trichomonas vaginalis. PMID: 22212642
Polyamines and their derivatives as modulators in growth and differentiation. PMID: 2697982
Differential expression of genes encoding the hypusine-containing translation initiation factor, eIF-5A, in tobacco. PMID: 1542563
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Product Notes
The Hypusine (Catalog #AAA120202) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hypusine antibody (Hpu24) reacts with General and may cross-react with other species as described in the data sheet. AAA Biotech's Hypusine can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the Hypusine for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hypusine, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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