Rat IFN-alpha Monoclonal Antibody | anti-IFN-alpha antibody

IFN-alpha, Mouse, mAb F18

Cat. Num. AAA77791

• Gene Names: IFNA1; IFL; IFN; IFNA@; IFNA13; IFN-ALPHA; IFN-alphaD
• Reactivity: Cross reactant: Human IFN Reactivity: No; Cross reactant: Mouse IFN-Beta Reactivity: No; Cross reactant: Mouse IFN-gamma Reactivity: No
• Synonyms: IFN-alpha, Antibody; IFN-alpha, Mouse, mAb F18; IFN-alpha; anti-IFN-alpha antibody

• Host: Rat
• Reactivity: Cross reactant: Human IFN Reactivity: No; Cross reactant: Mouse IFN-Beta Reactivity: No; Cross reactant: Mouse IFN-gamma Reactivity: No
• Clonality: Monoclonal
• Isotype: Rat IgG1
• Form/Format: 1 ml (100 ug/ml) 0.2 um filtered protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin. The endotoxin concentration is < 24 EU/mg, determined with MBS584532 LAL Assay; For unit size 0.5 mg or larger: PBS only

• Positive Control: Plasmacytoid dendritic cells
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.

Application Data

(IA: AAA77791 has been used as a capture antibody to determine)

Application Data

Related Product Information for anti-IFN-alpha antibody

The monoclonal antibody F18 recognizes and neutralizes both natural and recombinant mouse alpha Interferon (IFN-alpha). IFN-alpha is a cytokine that belongs to the type I interferons (IFN-I). IFN-alpha is secreted by many cell types including lymphocytes (NK cells, B-cells and T-cells), macrophages, fibroblasts, endothelial cells, osteoblasts, microglia and others. Interferons stimulate both macrophages and NK cells to elicit an anti-viral response, and are also active against tumors. Although all cells can produce IFN-I, plasmacytoid dendritic cells (pDCs) produce 1,000-fold higher levels than other cell types, and are responsible for systemic IFN-I responses to many viruses. They are coined as the natural IFN-producing cells. However, under deprived pDC condition, other dendritic cells are capable of producing high levels of IFN-I.

Interferons were initially characterized for their ability to 'interfere' with viral replication, slow cell proliferation, and profoundly alter immunity. IFN-alpha has several regulatory roles and diverse biological activities, including control of cellular and humoral immune responses, inflammation, and tumor regression. In addition, IFN-alpha participates in the regulation of various cellular and humoral processes such as the endocrine system modulates behavior, brain activity, temperature, glucose sensitive neurons, feeding pattern and opiate activity.
With the availability of monoclonal antibodies directed against IFN-alpha, it is possible to interpret results obtained from crude materials containing both IFN-alpha and IFNbeta. The difficulties in studying in vitro and in vivo effects of 'type 1'. Interferons arise from the fact that both alpha and beta Interferons are produced in response to the same stimuli and also seem to act via the same receptor. These Interferon activities can only be distinguished from one another by use of specific neutralizing antibodies.

References

1. Dalod, M et al; Interferon ;/β and interleukin 12 responses to viral infections: pathways regulating dendritic cell cytokine expression in vivo. J Exp Med 2002, 195: 517 2. Diebold, S et al; Viral infection switches nonplasmacytoid dendritic cells into high interferon producers. Nature 2003, 424: 324
3. Joshi, V et al; A role for Stat1 in the regulation of lipopolysaccharide-induced Interleukin-1beta expression. J Interferon Cytokine Res 2006, 26: 739.

NCBI and Uniprot Product Information

• NCBI GI #: 13128950
• NCBI GeneID: 3439
• NCBI Accession #: NP_076918.1
• NCBI GenBank Nucleotide #: NM_024013.2
• UniProt Accession #: P01562; Q14605; Q2M1L8; Q52LB8; Q5VYQ2; Q7M4Q1; Q8WZ68; Q9UMJ3; D4Q9M8
• Molecular Weight: 21,725 Da
• NCBI Official Full Name: interferon alpha-1/13
• NCBI Official Synonym Full Names: interferon alpha 1
• NCBI Official Symbol: IFNA1
• NCBI Official Synonym Symbols: IFL; IFN; IFNA@; IFNA13; IFN-ALPHA; IFN-alphaD
• NCBI Protein Information: interferon alpha-1/13
• UniProt Protein Name: Interferon alpha-1/13
• UniProt Gene Name: IFNA1
• UniProt Synonym Gene Names: IFN-alpha-1/13; LeIF D