FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Mouse IFNAR1 expression on BABL/c splenocytes. Cells were stained with Mouse BD Fc Block' purified anti-mouse CD16/CD32 mAb,then stained with purified anti-Mouse IFNAR1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Rabbit anti-Mouse IFNAR1 Monoclonal Antibody | anti-IFNAR1 antibody
Recombinant Anti-IFNAR1 Antibody, Rabbit Monoclonal
Gene Names
Ifnar1; Ifar; Ifrc; CD118; Ifnar; Infar
Reactivity
Mouse
Applications
Immunofluorescence, Immunocytochemistry, Flow Cytometry, Functional Assay
Purity
Protein A
Synonyms
IFNAR1, Antibody; Recombinant Anti-IFNAR1 Antibody, Rabbit Monoclonal; IFNAR1 Antibody, Rabbit MAb; interferon (alpha, beta and omega) receptor 1; Anti-CD118 Antibody; Anti-Ifar Antibody; Anti-Ifnar Antibody; Anti-Ifrc Antibody; Anti-Infar Antibody; anti-IFNAR1 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
110
Specificity
Mouse IFNAR1
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-IFNAR1 antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Mouse IFNAR1 Protein
Conjugation
Unconjugated
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse IFNAR1 (rM IFNAR1).
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Mouse IFNAR1 expression on BABL/c splenocytes. Cells were stained with Mouse BD Fc Block' purified anti-mouse CD16/CD32 mAb,then stained with purified anti-Mouse IFNAR1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Mouse IFNAR1 expression on BABL/c splenocytes. Cells were stained with Mouse BD Fc Block' purified anti-mouse CD16/CD32 mAb,then stained with purified anti-Mouse IFNAR1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
IF (Immunofluorescence)
(Immunofluorescence staining of mouse IFNAR1 in mouse splenocytes. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse IFNAR1 monoclonal antibody (1:60) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue).)
IF (Immunofluorescence)
(Immunofluorescence staining of mouse IFNAR1 in mouse splenocytes. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse IFNAR1 monoclonal antibody (1:60) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue).)
Related Product Information for anti-IFNAR1 antibody
Interferon-alpha/beta receptor alpha chain (IFNAR1) is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulate Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. The encoded protein also functions as an antiviral factor. Tyk2 slows down IFNAR1 degradation and that this is due, at least in part, to inhibition of IFNAR1 endocytosis. Mutant versions of IFNAR1, in which Tyr466 is changed to phenylalanine, can act in a dominant-negative manner to inhibit phosphorylation of STAT2. These observations are consistent with a model in which IFNAR1 mediates the interaction between JAK kinases and the STAT transcription factors.
References
Yan H, et al. (1996) Phosphorylated interferon-alpha receptor 1 subunit (IFNaR1) acts as a docking site for the latent form of the 113 kDa STAT2 protein. EMBO J. 15(5): 1064-74.Richter MF, et al. (1998) Specific contribution of Tyk2 JH regions to the binding and the expression of the interferon alpha/beta receptor component IFNAR1. J Biol Chem. 273(38): 24723-9.Abramovich C, et al. (1997) A protein-arginine methyltransferase binds to the intracytoplasmic domain of the IFNAR1 chain in the type I interferon receptor. EMBO J. 16(2): 260-6.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
65,796 Da
NCBI Official Full Name
interferon alpha/beta receptor 1
NCBI Official Synonym Full Names
interferon (alpha and beta) receptor 1
NCBI Official Symbol
Ifnar1
NCBI Official Synonym Symbols
Ifar; Ifrc; CD118; Ifnar; Infar
NCBI Protein Information
interferon alpha/beta receptor 1
UniProt Protein Name
Interferon alpha/beta receptor 1
UniProt Gene Name
Ifnar1
UniProt Synonym Gene Names
Ifar; Ifnar; IFN-R-1; IFN-alpha/beta receptor 1
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Product Notes
The IFNAR1 ifnar1 (Catalog #AAA257164) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-IFNAR1 Antibody, Rabbit Monoclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IFNAR1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the IFNAR1 ifnar1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IFNAR1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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