Application Data
(Purified Bovine IgA detected with Mouse anti Bovine/Ovine IgA followed by Rabbit F(ab')2 anti Mouse IgG:HRP)
Mouse anti-Bovine IgA Monoclonal Antibody | anti-IgA antibody
MOUSE ANTI BOVINE/OVINE IgA
Shelf Life: 18 months from date of despatch
Application Data
(Purified Bovine IgA detected with Mouse anti Bovine/Ovine IgA followed by Rabbit F(ab')2 anti Mouse IgG:HRP)
Application Data
(Purified Bovine IgA detected with Mouse anti Bovine/Ovine IgA followed by Rabbit F(ab')2 anti Mouse IgG:HRP)
Application Data
(Published customer image: Mouse anti Bovine IgA antibody, clone K84 2F9 used for the measurement of Tci-L4-ES-specific IgA responses in sheep by ELISA.Image caption:The effects of T. circumcincta infection on Tci-L4-ES mediated lymphocyte suppression.?Seven helminth-naïve lambs were trickle infected with 2000 T. circumcincta?L3 three times a week for four weeks. PBMC were harvested at 0, 2, 4 and 6 weeks from the start of infection and cultured for 72 h with PBS alone (PBS), 5 μg/mL Con A (Con A + PBS) or 5 μg/mL Con A + 15 ug/mL Tci-L4-ES (Con A + ES). Proliferation was assessed at 72 h by measurement of [3H] thymidine incorporation. Levels of cytokine present in the cell culture supernatants at 54 h were determined by ELISA. (A)?Faecal egg count (FEC) data over the course of the experimental infection. (B)?Proliferation of PBMC in response to PBS, Con A + PBS and Con A + ES during experimental T. circumcincta infection. (C)?Levels of serum Tci-L4-ES-specific IgA and IgG during experimental T. circumcincta?infection. (D-F) Concentrations of IL-4, IL-10 and IFN-gamma in PBMC cultures supernatants. Data represents mean +/- SEM. a?significant difference between Con A + PBS vs. PBS-stimulated cultures; b?significant difference between ConA + ES vs. PBS-stimulated cultures; c?significant difference between Con A + PBS vs. Con A + ES stimulated cultures; *P?< 0.05, ** P?< 0.01, ***P?< 0.001 (repeated-measures two-way ANOVA followed by the Bonferroni post hoc test for comparison of means).From: McNeilly TN, Rocchi M, Bartley Y, Brown JK, Frew D, Longhi C, McLean L, McIntyre J, Nisbet AJ, Wattegedera S, Huntley JF, Matthews JB. Suppression of ovine lymphocyte activation by Teladorsagia circumcincta larval excretory-secretory products.Vet Res. 2013 Aug 21;44:70..)
Application Data
(Published customer image: Mouse anti Bovine IgA antibody, clone K84 2F9 used for the measurement of Tci-L4-ES-specific IgA responses in sheep by ELISA.Image caption:The effects of T. circumcincta infection on Tci-L4-ES mediated lymphocyte suppression.?Seven helminth-naïve lambs were trickle infected with 2000 T. circumcincta?L3 three times a week for four weeks. PBMC were harvested at 0, 2, 4 and 6 weeks from the start of infection and cultured for 72 h with PBS alone (PBS), 5 μg/mL Con A (Con A + PBS) or 5 μg/mL Con A + 15 ug/mL Tci-L4-ES (Con A + ES). Proliferation was assessed at 72 h by measurement of [3H] thymidine incorporation. Levels of cytokine present in the cell culture supernatants at 54 h were determined by ELISA. (A)?Faecal egg count (FEC) data over the course of the experimental infection. (B)?Proliferation of PBMC in response to PBS, Con A + PBS and Con A + ES during experimental T. circumcincta infection. (C)?Levels of serum Tci-L4-ES-specific IgA and IgG during experimental T. circumcincta?infection. (D-F) Concentrations of IL-4, IL-10 and IFN-gamma in PBMC cultures supernatants. Data represents mean +/- SEM. a?significant difference between Con A + PBS vs. PBS-stimulated cultures; b?significant difference between ConA + ES vs. PBS-stimulated cultures; c?significant difference between Con A + PBS vs. Con A + ES stimulated cultures; *P?< 0.05, ** P?< 0.01, ***P?< 0.001 (repeated-measures two-way ANOVA followed by the Bonferroni post hoc test for comparison of means).From: McNeilly TN, Rocchi M, Bartley Y, Brown JK, Frew D, Longhi C, McLean L, McIntyre J, Nisbet AJ, Wattegedera S, Huntley JF, Matthews JB. Suppression of ovine lymphocyte activation by Teladorsagia circumcincta larval excretory-secretory products.Vet Res. 2013 Aug 21;44:70..)
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Product Notes
The IgA (Catalog #AAA50248) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI BOVINE/OVINE IgA reacts with Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's IgA can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the IgA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgA, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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