IF (Immunofluorescence)
(ICC/IF analysis of IGF1 in PC3 cells. The cell was stained with ATGA0311 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
Mouse anti-Human IGF1 Monoclonal Antibody | anti-IGF1 antibody
IGF1 antibody
Gene Names
IGF1; MGF; IGFI; IGF-I
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Western Blot, ELISA
Purity
By protein-A affinity chromatography
Synonyms
IGF1, Antibody; IGF1 antibody; Insulin-like growth factor-1; IGF-IA; Somatomedin-C; MGF; IBP1; IGF1; IGF-1; Insulin-like growth factor-1 IGF IA; IGF IB; IGFI; IGFIA; Insulin like growth factor 1; Insulin like growth factor 1 (somatomedin C); Insulin like growth factor IA; Insulin like growth factor IB; Mechano growth factor; Somatomedin C,; anti-IGF1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
AT6F8
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
153
Applicable Applications for anti-IGF1 antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA
Antigen Species
Human
Immunogen
Recombinant human IGF1 (49-118aa) purified from E. coli
Clone Note
Anti-human IGF1 mAb, clone AT6F8, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human IGF1 protein
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
IF (Immunofluorescence)
(ICC/IF analysis of IGF1 in PC3 cells. The cell was stained with ATGA0311 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
IF (Immunofluorescence)
(ICC/IF analysis of IGF1 in PC3 cells. The cell was stained with ATGA0311 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
WB (Western Blot)
(The human IGF1 recombinant protein (12.5ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IGF1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IGF1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: IGF1 transfected 293T cell lysate)
WB (Western Blot)
(The human IGF1 recombinant protein (12.5ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IGF1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IGF1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: IGF1 transfected 293T cell lysate)
Related Product Information for anti-IGF1 antibody
Insulin-like growth factor 1 (IGF-1), also called somatomedin C, is a protein that in humans is encoded by the IGF1 gene. IGF-1 has also been referred to as a Sulfation factor and its effects were termed nonsuppressible insulin-like activity (NSILA) in the 1970s. IGF-1 is a hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. A synthetic analog of IGF-1, mecasermin, is used for the treatment of growth failure. IGF-1 consists of 70 amino acids in a single chain with three intramolecular disulfide bridges. IGF-1 has a molecular weight of 7,649 daltons.
Product Categories/Family for anti-IGF1 antibody
References
Hoppener J.W., et al. (1985) Hum Genet 69 (2): 157-160; Jansen M., et al. (1983) Nature 306 (5943): 609-611; Salmon W., et al. (1957) J Lab Clin Med 49 (6): 825-836 ; Keating G.M., (2008) BioDrugs 22 (3): 177-188; Rinderknecht E., et al. (1978) J Biol Chem 253 (8): 2769-2776
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,762 Da
NCBI Official Full Name
insulin-like growth factor I isoform 4 preproprotein
NCBI Official Synonym Full Names
insulin like growth factor 1
NCBI Official Symbol
IGF1
NCBI Official Synonym Symbols
MGF; IGFI; IGF-I
NCBI Protein Information
insulin-like growth factor I
UniProt Protein Name
Insulin-like growth factor I
UniProt Gene Name
IGF1
UniProt Synonym Gene Names
IBP1; IGF-I; MGF
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Product Notes
The IGF1 igf1 (Catalog #AAA48141) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The IGF1 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IGF1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the IGF1 igf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IGF1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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