Mouse IgG4 (Ig Heavy Constant Gamma 4) Monoclonal Antibody | anti-IGHG4 antibody

IgG4 (Ig Heavy Constant Gamma 4) (G4m Marker)

Cat. Num. AAA214395

• Reactivity: Human. Others not known.
• Applications: Immunohistochemistry, Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
• Synonyms: IgG4 (Ig Heavy Constant Gamma 4), Antibody; IgG4 (Ig Heavy Constant Gamma 4) (G4m Marker); Ig gamma 4 chain C region; IGHG4; Immunoglobulin heavy constant gamma 4 (G4m marker); anti-IGHG4 antibody

• Host: Mouse
• Reactivity: Human. Others not known.
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: rIGHG4/1345
• Specificity: The regions of relatively constant sequence beyond the variable regions of immunoglobulins are termed constant regions (C regions) and are present in both the heavy and light chains. With very few exceptions, the sites of attachment for carbohydrates on immunoglobulins are located in these C regions. These regions also function to hold the variable regions together by using the disulfide bond between them. The C regions facilitate interaction with the antigen by increasing the maximum rotation of the immunoglobulin arms. Reportedly, a large population of patients with recurrent respiratory tract infection has low IgG4 concentrations. IgG4-related sclerosing disease has been recognized as a systemic disease entity characterized by an elevated serum IgG4 level, sclerosing fibrosis, and diffuse lymphoplasmacytic infiltration with the presence of many IgG4-positive plasma cells. IgG4 is overexpressed in inflammatory pseudotumor (IPT) and under expressed in inflammatory myofibroblastic tumor (IMT). In pulmonary nodular lymphoid hyperplasia (PNLH), there are an increased number of IgG4+ plasma cells.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 327

• Applicable Applications for anti-IGHG4 antibody: IHC (Immunohistochemistry), WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
• Immunogen: Recombinant full-length human IGHG4 fragment
• Cellular Localization: Cytoplasmic
• Hu-chromosome Location: 14q32.33
• Positive Control: Tonsil
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C.; Antibody without azide - store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-PAGE

(SDS-PAGE Analysis Purified IgG4 Mouse Recombinant Monoclonal Antibody (rIGHG4/1345).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded Human Tonsil stained with IgG4 Mouse Recombinant Monoclonal Antibody (rIGHG4/1345).)

References

Adetugbo, K., et al. 1978. Evolution of immunoglobulin subclasses. Primary structure of a murine myeloma ?1 chain. J. Biol. Chem. 253: 6068-6075.

NCBI and Uniprot Product Information

• NCBI GI #: 121047
• NCBI GeneID: 3503
• UniProt Accession #: P01861; P55317
• Molecular Weight: 150kDa
• NCBI Official Full Name: Immunoglobulin heavy constant gamma 4
• NCBI Official Synonym Full Names: immunoglobulin heavy constant gamma 4 (G4m marker)
• NCBI Official Symbol: IGHG4
• UniProt Protein Name: Immunoglobulin heavy constant gamma 4
• UniProt Gene Name: IGHG4