WB (Western Blot)
(Detection of human IgG4 by western blot. Samples: Purified human IgG1 (0.1 ug), IgG2 (0.5 ug), IgG3 (0.5 ug), and IgG4 (0.5 ug). Antibody: Rabbit anti-Human IgG4 recombinant monoclonal antibody (AAA213584 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Goat anti-Human IgG-heavy and light chain antibody .)
IgG4 recombinant antibody
Rabbit anti-Human IgG4 Recombinant Monoclonal Antibody [BLR113H]
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified
Synonyms
IgG4, Recombinant Antibody; Rabbit anti-Human IgG4 Recombinant Monoclonal Antibody [BLR113H]; hIgG4; immunoglobulin heavy constant gamma 4 (G4m marker); IgG4 recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BLR113H
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
100ug/ml (varies by lot)
Applicable Applications for IgG4 recombinant antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Between 75 and 125
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human IgG4 by western blot. Samples: Purified human IgG1 (0.1 ug), IgG2 (0.5 ug), IgG3 (0.5 ug), and IgG4 (0.5 ug). Antibody: Rabbit anti-Human IgG4 recombinant monoclonal antibody (AAA213584 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Goat anti-Human IgG-heavy and light chain antibody .)
WB (Western Blot)
(Detection of human IgG4 by western blot. Samples: Purified human IgG1 (0.1 ug), IgG2 (0.5 ug), IgG3 (0.5 ug), and IgG4 (0.5 ug). Antibody: Rabbit anti-Human IgG4 recombinant monoclonal antibody (AAA213584 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Goat anti-Human IgG-heavy and light chain antibody .)
IHC (Immunohistochemistry)
(Detection of human IgG4 in FFPE tonsil by immunohistochemistry. Antibody: Rabbit anti-Human IgG4 recombinant monoclonal antibody (AAA213584 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry)
(Detection of human IgG4 in FFPE tonsil by immunohistochemistry. Antibody: Rabbit anti-Human IgG4 recombinant monoclonal antibody (AAA213584 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
Related Product Information for IgG4 recombinant antibody
IgG4 represents only 2-5% of the human IgG subclasses in serum. IgG4 lacks effector functions, such as antibody-dependent cell-mediated cytotoxicity and complement-dependent cytotoxicity. IgG4 plays a protective role in allergy by acting as a blocking antibody, and inhibiting mast cell degranulation, but a deleterious role in malignant melanoma, by impeding IgG1-mediated anti-tumor immunity.
Product Categories/Family for IgG4 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
146,000
NCBI Official Full Name
Ig gamma-4 chain C region
NCBI Official Synonym Full Names
immunoglobulin heavy constant gamma 4 (G4m marker)
NCBI Official Symbol
IGHG4
UniProt Protein Name
Ig gamma-4 chain C region
UniProt Gene Name
IGHG4
UniProt Entry Name
IGHG4_HUMAN
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Product Notes
The IgG4 ighg4 (Catalog #AAA213584) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Human IgG4 Recombinant Monoclonal Antibody [BLR113H] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IgG4 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the IgG4 ighg4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgG4, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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