SDS-PAGE
(Fig. 3: Coomassie staining of SDS-PAGE gel loaded with Anti-IL-1beta (Canakinumab biosimilar) mAb.)
Human anti-Human IL-1 beta Monoclonal Antibody | anti-IL-1beta antibody
Recombinant anti-human-IL-1 beta Antibody (Canakinumab Biosimilar)
Gene Names
IL1B; IL-1; IL1F2; IL1-BETA
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunocytochemistry, ELISA, Functional Assay
Purity
Purified from cell culture supernatant by affinity chromatography
Synonyms
IL-1 beta, Antibody; Recombinant anti-human-IL-1 beta Antibody (Canakinumab Biosimilar); Interleukin-1 beta; Catabolin; IL-1 Beta; Canakinumab; anti-IL-1beta antibody
Host
Human
Reactivity
Human
Clonality
Monoclonal
Isotype
Human IgG1
Specificity
Human IL1 beta. Does not cross react with other members of the interleukin-1 family.
Purity/Purification
Purified from cell culture supernatant by affinity chromatography
Form/Format
Purified. Lyophilized from sterile PBS, pH7.4. Normally 5 %-8% trehalose is added as protectants before lyophilization.Not Sterile
Applicable Applications for anti-IL-1beta antibody
FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ICC (Immunocytochemistry), ELISA, FA (Functional Assay)
Source
Recombinant in Human cells
Preparation and Storage
Store at -20 degree C (Avoid repeated freezing and thawing)
SDS-PAGE
(Fig. 3: Coomassie staining of SDS-PAGE gel loaded with Anti-IL-1beta (Canakinumab biosimilar) mAb.)
SDS-PAGE
(Fig. 3: Coomassie staining of SDS-PAGE gel loaded with Anti-IL-1beta (Canakinumab biosimilar) mAb.)
FCM/FACS (Flow Cytometry)
(Figure 2. Flow cytometry analysis with 1ug/mL Anti-IL1B (canakinumab biosimilar) mAb (AAA78589) on Expi293 cells transfected with Human IL1B protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).)
FCM/FACS (Flow Cytometry)
(Figure 2. Flow cytometry analysis with 1ug/mL Anti-IL1B (canakinumab biosimilar) mAb (AAA78589) on Expi293 cells transfected with Human IL1B protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).)
ELISA
(Figure 1. ELISA plate pre-coated by 1ug/mL (100 ul/well) Human IL1B Protein, hFc Tag can bind Anti-IL1B Neutralizing antibody (AAA78589) in a linear range of 0.61-156.25ng/mL.)
ELISA
(Figure 1. ELISA plate pre-coated by 1ug/mL (100 ul/well) Human IL1B Protein, hFc Tag can bind Anti-IL1B Neutralizing antibody (AAA78589) in a linear range of 0.61-156.25ng/mL.)
Related Product Information for anti-IL-1beta antibody
Canakinumab is a recombinant, human anti-human-IL-1beta monoclonal antibody that belongs to the IgG1/kappa isotype subclass with a molecular mass of 145157 Daltons when deglycosylated. Canakinumab binds to human IL-1beta and neutralizes its inflammatory activity by blocking its interaction with IL-1 receptors, but it does not bind IL-1alpha or IL-1 receptor antagonist (IL-1ra).
Product Categories/Family for anti-IL-1beta antibody
References
1. Church LD, McDermott MF: Canakinumab, a fully-human mAb against IL-1beta for the potential treatment of inflammatory disorders. Curr Opin Mol Ther. 2009 Feb;11(1):81-89.
2. Lachmann HJ, et al.: Use of canakinumab in the cryopyrin-associated periodic syndrome. N Engl J Med. 2009 Jun 4;360(23):2416-25. doi:10.1056/NEJMoa0810787
2. Lachmann HJ, et al.: Use of canakinumab in the cryopyrin-associated periodic syndrome. N Engl J Med. 2009 Jun 4;360(23):2416-25. doi:10.1056/NEJMoa0810787
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated: 30 kDa; Observed: 36 kDa
NCBI Official Full Name
interleukin-1 beta proprotein
NCBI Official Synonym Full Names
interleukin 1, beta
NCBI Official Symbol
IL1B
NCBI Official Synonym Symbols
IL-1; IL1F2; IL1-BETA
NCBI Protein Information
interleukin-1 beta; IL-1 beta; catabolin; preinterleukin 1 beta; pro-interleukin-1-beta
UniProt Protein Name
Interleukin-1 beta
UniProt Gene Name
IL1B
UniProt Synonym Gene Names
IL1F2; IL-1 beta
UniProt Entry Name
IL1B_HUMAN
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Product Notes
The IL-1beta il1b (Catalog #AAA78589) is an Antibody produced from Human and is intended for research purposes only. The product is available for immediate purchase. The Recombinant anti-human-IL-1 beta Antibody (Canakinumab Biosimilar) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IL-1 beta can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ICC (Immunocytochemistry), ELISA, FA (Functional Assay). Researchers should empirically determine the suitability of the IL-1beta il1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-1 beta, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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