FCM/FACS (Flow Cytometry)
(Flowcytometryusinganti-IL-12/2antibodyABT-874(Briakinumab)(AAA72491). Paraformaldehyde-fixedJurkatcellspermeabilizedwith0.5%Tritonwerestainedwiththeanti-unknownspecificityantibodyortherabbitIgGversionofABT-874(AAA72491,blueline)atadilutionof1:100for1hatRT.Afterwashing,theboundantibodywasdetectedusingagoatanti-rabbitIgGAlexaFluor488antibodyatadilutionof1:1000,andthecellswereanalyzedusingaFACSCantoflow-cytometer.)
IL-12/23 recombinant antibody
Anti-IL-12/23 [ABT-874 (Briakinumab)], Human IgG1, lambda
Gene Names
IL12B; CLMF; NKSF; CLMF2; NKSF2; IL-12B
Reactivity
Human
Applications
Blocking
Purity
Protein A affinity purified
Synonyms
IL-12/23, Recombinant Antibody; Anti-IL-12/23 [ABT-874 (Briakinumab)], Human IgG1, lambda; IL-12/23; Anti-IL-12/23 [ABT-874 (Briakinumab)]; IL-12p40; Interleukin 12; Interleukin 23; IL12; IL23; IL-12; IL-23; IL-12/23 recombinant antibody
Host
Human
Reactivity
Human
Clonality
Monoclonal
Isotype
Human IgG1 lambda
Clone Number
ABT-874 (Briakinumab)
Specificity
Binds to human IL12 and IL23 via IL-12/23p40.
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for IL-12/23 recombinant antibody
Blocking
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flowcytometryusinganti-IL-12/2antibodyABT-874(Briakinumab)(AAA72491). Paraformaldehyde-fixedJurkatcellspermeabilizedwith0.5%Tritonwerestainedwiththeanti-unknownspecificityantibodyortherabbitIgGversionofABT-874(AAA72491,blueline)atadilutionof1:100for1hatRT.Afterwashing,theboundantibodywasdetectedusingagoatanti-rabbitIgGAlexaFluor488antibodyatadilutionof1:1000,andthecellswereanalyzedusingaFACSCantoflow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flowcytometryusinganti-IL-12/2antibodyABT-874(Briakinumab)(AAA72491). Paraformaldehyde-fixedJurkatcellspermeabilizedwith0.5%Tritonwerestainedwiththeanti-unknownspecificityantibodyortherabbitIgGversionofABT-874(AAA72491,blueline)atadilutionof1:100for1hatRT.Afterwashing,theboundantibodywasdetectedusingagoatanti-rabbitIgGAlexaFluor488antibodyatadilutionof1:1000,andthecellswereanalyzedusingaFACSCantoflow-cytometer.)
IF (Immunofluorescence)
(ImmunofluorescencestainingofJurkatcellswithanti-IL-12/23antibodyABT-874(Briakinumab)(AAA72491). ImmunofluorescenceanalysisofparaformaldehydefixedJurkatcellsonShi-fixcoverslipsstainedwiththechimericrabbitIgGversionofABT-874(Briakinumab)(AAA72491)(1:100dilution)for1hfollowedbyAlexaFluor488secondaryantibody(1:1000dilution),showingmembraneandcytoplasmicstaining.ThenuclearstainisDAPI(blue).Panelsshow,fromleft-right,top-bottom,AAA72491,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
IF (Immunofluorescence)
(ImmunofluorescencestainingofJurkatcellswithanti-IL-12/23antibodyABT-874(Briakinumab)(AAA72491). ImmunofluorescenceanalysisofparaformaldehydefixedJurkatcellsonShi-fixcoverslipsstainedwiththechimericrabbitIgGversionofABT-874(Briakinumab)(AAA72491)(1:100dilution)for1hfollowedbyAlexaFluor488secondaryantibody(1:1000dilution),showingmembraneandcytoplasmicstaining.ThenuclearstainisDAPI(blue).Panelsshow,fromleft-right,top-bottom,AAA72491,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
Related Product Information for IL-12/23 recombinant antibody
This antibody was generated by phage display technology.
References
Sandborn How future tumor necrosis factor antagonists and other compounds will meet the remaining challenges in Crohn's disease. Rev Gastroenterol Disord. 2004;4 Suppl 3:S25-33. N/A15583528
Note on publication:
Discusses the potential of this antibody in the treatment of Crohn's disease.
Note on publication:
Discusses the potential of this antibody in the treatment of Crohn's disease.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
328
NCBI Official Full Name
interleukin-12 subunit beta
NCBI Official Synonym Full Names
interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
NCBI Official Symbol
IL12B
NCBI Official Synonym Symbols
CLMF; NKSF; CLMF2; NKSF2; IL-12B
NCBI Protein Information
interleukin-12 subunit beta; CLMF p40; IL-12 subunit p40; IL12, subunit p40; interleukin 12, p40; interleukin-12 beta chain; NK cell stimulatory factor chain 2; cytotoxic lymphocyte maturation factor 40 kDa subunit; natural killer cell stimulatory factor,
UniProt Protein Name
Interleukin-12 subunit beta
UniProt Gene Name
IL12B
UniProt Synonym Gene Names
NKSF2; IL-12B; CLMF p40; NKSF2
UniProt Entry Name
IL12B_HUMAN
Similar Products
Product Notes
The IL-12/23 il12b (Catalog #AAA72491) is a Recombinant Antibody produced from Human and is intended for research purposes only. The product is available for immediate purchase. The Anti-IL-12/23 [ABT-874 (Briakinumab)], Human IgG1, lambda reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IL-12/23 can be used in a range of immunoassay formats including, but not limited to, Blocking. Researchers should empirically determine the suitability of the IL-12/23 il12b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-12/23, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.