WB (Western Blot)
(Detection of human IL-2 by western blot. Samples: Whole cell lysate (50 ug) from Jurkat cells untreated (-) or treated (+) with 40nM TPA, 2uM A23187, and 300ng/mL Brefeldin A. Antibody: Rabbit anti-IL-2 recombinant monoclonal antibody (AAA213687 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
IL-2 recombinant antibody
Rabbit anti-IL-2 Recombinant Monoclonal Antibody [BLR287L]
Gene Names
IL2; IL-2; TCGF; lymphokine
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
IL-2, Recombinant Antibody; Rabbit anti-IL-2 Recombinant Monoclonal Antibody [BLR287L]; interleukin-2; TCGF; T-cell growth factor; T cell growth factor; involved in regulation of T-cell clonal expansion; IL-2; aldesleukin; lymphokine; Interleukin-2; interleukin 2; IL-2 recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BLR287L
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
Concentration
1000ug/ml (varies by lot)
Applicable Applications for IL-2 recombinant antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Residues 21-153
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human IL-2 by western blot. Samples: Whole cell lysate (50 ug) from Jurkat cells untreated (-) or treated (+) with 40nM TPA, 2uM A23187, and 300ng/mL Brefeldin A. Antibody: Rabbit anti-IL-2 recombinant monoclonal antibody (AAA213687 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot)
(Detection of human IL-2 by western blot. Samples: Whole cell lysate (50 ug) from Jurkat cells untreated (-) or treated (+) with 40nM TPA, 2uM A23187, and 300ng/mL Brefeldin A. Antibody: Rabbit anti-IL-2 recombinant monoclonal antibody (AAA213687 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
IP (Immunoprecipitation)
(Detection of human IL-2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells treated with 40nM TPA, 2uM A23187, and 300ng/mL Brefeldin A. Antibodies: Rabbit anti-IL-2 recombinant monoclonal antibody (AAA213687 lot 1) used for IP at 6 ul/mg lysate. IL-2 was also immunoprecipitated by a second antibody against a different epitope of IL-2 (Antibody 2). For blotting immunoprecipitated IL-2, AAA213687 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human IL-2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells treated with 40nM TPA, 2uM A23187, and 300ng/mL Brefeldin A. Antibodies: Rabbit anti-IL-2 recombinant monoclonal antibody (AAA213687 lot 1) used for IP at 6 ul/mg lysate. IL-2 was also immunoprecipitated by a second antibody against a different epitope of IL-2 (Antibody 2). For blotting immunoprecipitated IL-2, AAA213687 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for IL-2 recombinant antibody
IL2 is a member of the interleukin cytokine subfamily which includes IL4, IL7, IL9, IL15, IL21, erythropoietin, and thrombopoietin. IL2 is a secreted cytokine produced by activated CD4+ and CD8+ T lymphocytes, that is important for the proliferation of T and B lymphocytes. The IL2 receptor is a heterotrimeric protein complex whose gamma chain is also shared by IL4 and IL7. [taken from NCBI Entrez Gene (Gene ID: 3558)].
Product Categories/Family for IL-2 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,628 Da
NCBI Official Full Name
interleukin-2
NCBI Official Synonym Full Names
interleukin 2
NCBI Official Symbol
IL2
NCBI Official Synonym Symbols
IL-2; TCGF; lymphokine
NCBI Protein Information
interleukin-2; aldesleukin; T cell growth factor; involved in regulation of T-cell clonal expansion
UniProt Protein Name
Interleukin-2
UniProt Gene Name
IL2
UniProt Synonym Gene Names
IL-2; TCGF
UniProt Entry Name
IL2_HUMAN
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Product Notes
The IL-2 il2 (Catalog #AAA213687) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-IL-2 Recombinant Monoclonal Antibody [BLR287L] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IL-2 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the IL-2 il2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-2, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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