Mouse anti-Rat IL-3 Monoclonal Antibody | anti-IL-3 antibody

Anti-IL-3 Antibody, Mouse Monoclonal

Cat. Num. AAA259149

• Reactivity: Rat
• Applications: Immunoprecipitation, Western Blot
• Purity: Protein A; The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Synonyms: IL-3, Antibody; Anti-IL-3 Antibody, Mouse Monoclonal; interleukin 3; anti-IL-3 antibody

• Host: Mouse
• Reactivity: Rat
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: #50
• Specificity: Rat IL-3
• Purity/Purification: Protein A; The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-IL-3 antibody: IP (Immunoprecipitation), WB (Western Blot)
• Immunogen: Recombinant Rat IL3 Protein
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Rat IL3 (rR IL3).
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

IP (Immunoprecipitation)

(IL3 was immunoprecipitated using:Lane A:0.5 mg A431 Whole Cell Lysate4 uL anti-IL3 mouse monoclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-IL3 mouse monoclonal antibody,at 1:100 dilutionSecondary antibody:Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 17 kDaObserved band size :18 kDa)

WB (Western Blot)

(Anti-IL3 mouse monoclonal antibody at 1:500 dilutionLane A: Rat heart tissue lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:17 kDaObserved band size:17 kDa)

Related Product Information for anti-IL-3 antibody

IL3 (interleukin 3), also known as IL-3, is a potent growth-promoting cytokine that belongs to the IL-3 family. IL3/IL-3 also belongs to the group of interleukins. Interleukins are produced by a wide variety of body cells. The function of the immune system depends in a large part on interleukins, and rare deficiencies of a number of them have been described, all featuring autoimmune diseases or immune deficiency. The majority of interleukins are synthesized by helper CD4+ T lymphocytes, as well as through monocytes, macrophages, and endothelial cells. They promote the development and differentiation of T, B, and hematopoietic cells. IL3/IL-3 is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation, and apoptosis. IL3/IL-3 has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.

References

Meyer CG, et al.. (2011) IL3 variant on chromosomal region 5q31-33 and protection from recurrent malaria attacks. Hum Mol Genet. 20(6):1173-81.
Zambrano A, et al. (2010) IL-3 controls tau modifications and protects cortical neurons from neurodegeneration. Curr Alzheimer Res. 7(7):615-24.
Dentelli P, et al.. (2011) IL-3 is a novel target to interfere with tumor vasculature. Oncogene. 30(50):4930-40.