FCM/FACS (Flow Cytometry)
(Flowcytometryusinganti-IL-4antibodySB240683(Pascolizumab,humanized3B9)(AAA72530). Humanbloodleukocytesrestedfor4hoursat37°C,thenfixedwith2%PFAandpermeabilizedwith0.5%Triton,werestainedwiththeanti-unknownspecificityantibodyortherabbitIgGversionofSB240683(AAA72530,left)atadilutionof1:100overnightat4°C.Afterwashing,theboundantibodywasdetectedusingagoatanti-rabbitIgGAlexaFluor488antibodyatadilutionof1:1000,andthecellswereanalyzedusingaFACSCantoflow-cytometer.)
IL-4 recombinant antibody
Anti-IL-4 [SB240683 (Pascolizumab, humanized 3B9)], Human IgG1, kappa
Gene Names
IL4; BSF1; IL-4; BCGF1; BSF-1; BCGF-1
Reactivity
Human, cynomolgus monkeys
Applications
Functional Assay, ELISA
Purity
Protein A affinity purified
Synonyms
IL-4, Recombinant Antibody; Anti-IL-4 [SB240683 (Pascolizumab, humanized 3B9)], Human IgG1, kappa; IL-4; Anti-IL-4 [SB240683 (Pascolizumab, humanized 3B9)]; IL4; BSF-1; Interleukin-4; B-cell stimulatory factor 1; Binetrakin; Lymphocyte stimulatory factor 1; Pitrakinra; IL-4 recombinant antibody
Host
Human
Reactivity
Human, cynomolgus monkeys
Clonality
Monoclonal
Isotype
Human IgG1 kappa
Clone Number
SB240683 (Pascolizumab, humanized 3B9)
Specificity
This antibody binds human and cynomolgus monkey IL-4 and does not cross react with IL-4 from mouse, rat, cow, goat or horse. It does show binding to glycosylated interleukin-4. Interleukin-4 is a receptor for both interleukin 4 and interleukin 13. It couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2.
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for IL-4 recombinant antibody
FA (Functional Assay), Blocking, IA (Inhibition Assay), ELISA
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flowcytometryusinganti-IL-4antibodySB240683(Pascolizumab,humanized3B9)(AAA72530). Humanbloodleukocytesrestedfor4hoursat37°C,thenfixedwith2%PFAandpermeabilizedwith0.5%Triton,werestainedwiththeanti-unknownspecificityantibodyortherabbitIgGversionofSB240683(AAA72530,left)atadilutionof1:100overnightat4°C.Afterwashing,theboundantibodywasdetectedusingagoatanti-rabbitIgGAlexaFluor488antibodyatadilutionof1:1000,andthecellswereanalyzedusingaFACSCantoflow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flowcytometryusinganti-IL-4antibodySB240683(Pascolizumab,humanized3B9)(AAA72530). Humanbloodleukocytesrestedfor4hoursat37°C,thenfixedwith2%PFAandpermeabilizedwith0.5%Triton,werestainedwiththeanti-unknownspecificityantibodyortherabbitIgGversionofSB240683(AAA72530,left)atadilutionof1:100overnightat4°C.Afterwashing,theboundantibodywasdetectedusingagoatanti-rabbitIgGAlexaFluor488antibodyatadilutionof1:1000,andthecellswereanalyzedusingaFACSCantoflow-cytometer.)
IF (Immunofluorescence)
(ImmunofluorescencestainingofDaudicellswithanti-IL-4antibodySB240683(Pascolizumab,humanized3B9)(AAA72530). ImmunofluorescenceanalysisofparaformaldehydefixedDaudicellsonShi-fixcoverslipsstainedwiththechimericrabbitIgGversionofSB240683(Pascolizumab,humanized3B9)(AAA72530)(1:100dilution)for1hfollowedbyAlexaFluor488secondaryantibody(1:1000dilution),showingcellularjunctionstaining.ThenuclearstainisDAPI(blue).Panelsshow,fromleft-right,top-bottom,AAA72530,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
IF (Immunofluorescence)
(ImmunofluorescencestainingofDaudicellswithanti-IL-4antibodySB240683(Pascolizumab,humanized3B9)(AAA72530). ImmunofluorescenceanalysisofparaformaldehydefixedDaudicellsonShi-fixcoverslipsstainedwiththechimericrabbitIgGversionofSB240683(Pascolizumab,humanized3B9)(AAA72530)(1:100dilution)for1hfollowedbyAlexaFluor488secondaryantibody(1:1000dilution),showingcellularjunctionstaining.ThenuclearstainisDAPI(blue).Panelsshow,fromleft-right,top-bottom,AAA72530,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
Related Product Information for IL-4 recombinant antibody
The original mouse antibody was generated by immunizing F1 hybrids of Balb/c and C57BL/6 mice with recombinant human IL-4 in Freunds complete adjuvant. The humanized version was generated by grafting CDRs of murine parent antibody onto human IgG1 kappa heavy- and light-chain frameworks.
References
Hart et al. Preclinical efficacy and safety of pascolizumab (SB 240683): a humanized anti-interleukin-4 antibody with therapeutic potential in asthma. Clin Exp Immunol. 2002 Oct;130(1):93-100. N/A12296858
Note on publication:
The preclinical safety and efficacy of humanized version of anti-IL-4 antibody 3B9 (SB240683, Pascolizumab) was tested.
Note on publication:
The preclinical safety and efficacy of humanized version of anti-IL-4 antibody 3B9 (SB240683, Pascolizumab) was tested.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,492 Da
NCBI Official Full Name
interleukin-4 isoform 1
NCBI Official Synonym Full Names
interleukin 4
NCBI Official Symbol
IL4
NCBI Official Synonym Symbols
BSF1; IL-4; BCGF1; BSF-1; BCGF-1
NCBI Protein Information
interleukin-4; binetrakin; pitrakinra; B cell growth factor 1; B_cell stimulatory factor 1; lymphocyte stimulatory factor 1
UniProt Protein Name
Interleukin-4
UniProt Gene Name
IL4
UniProt Synonym Gene Names
IL-4; BSF-1
UniProt Entry Name
IL4_HUMAN
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Product Notes
The IL-4 il4 (Catalog #AAA72530) is a Recombinant Antibody produced from Human and is intended for research purposes only. The product is available for immediate purchase. The Anti-IL-4 [SB240683 (Pascolizumab, humanized 3B9)], Human IgG1, kappa reacts with Human, cynomolgus monkeys and may cross-react with other species as described in the data sheet. AAA Biotech's IL-4 can be used in a range of immunoassay formats including, but not limited to, FA (Functional Assay), Blocking, IA (Inhibition Assay), ELISA. Researchers should empirically determine the suitability of the IL-4 il4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-4, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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