anti-Mouse IL10 Monoclonal Antibody | anti-IL10 antibody

Rat Monoclonal [clone JES5-2A5] (IgG1) to Mouse IL10

Cat. Num. AAA51934

• Gene Names: IL10; CSIF; TGIF; GVHDS; IL-10; IL10A
• Reactivity: Mouse
• Applications: ELISA, Flow Cytometry, Functional Assay, Western Blot, Immunohistochemistry, Immunohistochemistry, Immunohistochemistry
• Purity: Protein G Purified
• Synonyms: IL10, Antibody; Rat Monoclonal [clone JES5-2A5] (IgG1) to Mouse IL10; Anti-IL10 Antibody (clone JES5-2A5) IHC-plus; IL10; CSIF; GVHDS; IL-10; IL10A; Interleukin-10; Interleukin 10; TGIF; Mouse IL10; anti-IL10 antibody

• Host: IL-10 antibody was produced in Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: JES5-2A5
• Specificity: A recognizes mouse Cytokine synthesis inhibitory factor(CSIF), also known, as interleukin IL-10). IL-10 is produces by Ly-1 (CD-5) expressing B cells and a subset of stimulated T cells. IL-10 has a wide range of biological properties, including the inhibition of macrophage and T helper cell mediated cytokine synthesis and the suspension of the delayed hypersensitivity response. Is reported to neutralize the activity of murine IL-10.
• Purity/Purification: Protein G Purified
• Form/Format: Tissue Culture supernatant, PBS , 0.09% Sodium Azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 178

• Applicable Applications for anti-IL10 antibody: ELISA, FCM/FACS (Flow Cytometry), WB (Western Blot), IHC (Immunohistochemistry), IHC (Immunohistochemistry), IHC (Immunohistochemistry)
• Target: IL-10
• Family/Subfamily: Hormone/not assigned- Hormone
• Immunogen Species: IL-10 antibody was raised againts Mouse.
• Immunogen: IL-10 antibody was raised against recombinant mouse IL-10.
• Antigen Type: Recombinant protein
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Note: During shipment,small volumes of product will ocassionally become entrapped in the seal of the product vial. We recommend briefly centrifuging the vial to dislodge any liquid in the container's cap prior to opening.
• Warning: This reagent may contain sodium azide. The chemical, physical, and toxicologial properties of this material have not been thoughly investigated.Standard Laboratory Practices should be followed. Avoid skin and eye contact,inhalation, and ingestion. Sodium azide forms hydrazoic acid under acidic conditions and may react with lead or copper plubing to form highly explosive metal azides. On disposal, flush with large volumes of water to prevent accumulation.
• Preparation and Storage: Store at +4°C or -20°C. Avoid repeated freezing and thawing.

WB (Western Blot)

(Western blot of mouse IL-10 recombinant protein probed with Rat anti-Mouse Interleukin-10 () followed by F(ab)2 Rabbit anti-Rat IgG:HRP (STAR21B).)

IHC (Immunohiostchemistry)

(Anti-IL-10 antibody IHC of mouse spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

IHC (Immunohistochemistry)

(Anti-IL-10 antibody IHC of mouse small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Related Product Information for anti-IL10 antibody

Interleukin 10 (IL1), a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes, has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway.

Product Categories/Family for anti-IL10 antibody

Family: Hormone

NCBI and Uniprot Product Information

• NCBI GI #: 10835141
• NCBI GeneID: 3586
• NCBI Accession #: NP_000563.1
• NCBI GenBank Nucleotide #: NM_000572.2
• UniProt Accession #: P22301
• NCBI Official Full Name: interleukin-10
• NCBI Official Synonym Full Names: interleukin 10
• NCBI Official Symbol: IL10
• NCBI Official Synonym Symbols: CSIF; TGIF; GVHDS; IL-10; IL10A
• NCBI Protein Information: interleukin-10; T-cell growth inhibitory factor; cytokine synthesis inhibitory factor
• UniProt Protein Name: Interleukin-10
• UniProt Gene Name: IL10
• UniProt Synonym Gene Names: IL-10; CSIF
• UniProt Entry Name: IL10_HUMAN