WB (Western Blot)
(IL1B Antibody (Center)(Ascites). 293 cell lysates transiently transfected with the IL1B gene.)
Mouse IL1B Monoclonal Antibody | anti-IL1B antibody
IL1B Antibody (Center) (Ascites)
Gene Names
IL1B; IL-1; IL1F2; IL1-BETA
Reactivity
Human (Predicted Reactivity: Monkey, Rabbit)
Applications
ELISA, Western Blot
Synonyms
IL1B, Antibody; IL1B Antibody (Center) (Ascites); Interleukin-1 beta; IL-1 beta; Catabolin; IL1B; IL1F2; anti-IL1B antibody
Host
Mouse
Reactivity
Human (Predicted Reactivity: Monkey, Rabbit)
Clonality
Monoclonal
Isotype
IgG1
Clone Number
614CT4.3.1
Specificity
This IL1B antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 148-174 amino acids from the Central region of human IL1B.
Form/Format
Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
Sequence Positions
148-174
Sequence Length
269
Applicable Applications for anti-IL1B antibody
ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Cellular location
Cytoplasm, cytosol. Lysosome. Secreted, exosome {ECO:0000250|UniProtKB:P10749}. Cytoplasmic vesicle, autophagosome. Secreted Note=The precursor is cytosolic. In response to inflammasome- activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted IL1B lacks any known signal sequence and the pathway(s) of its secretion is(are) not yet fully understood (PubMed:24201029). On the basis of experimental results, several unconventional secretion mechanisms have been proposed. 1. Secretion via secretory lysosomes: a fraction of CASP1 and IL1B precursor may be incorporated, by a yet undefined mechanism, into secretory lysosomes that undergo Ca(2+)-dependent exocytosis with release of mature IL1B (PubMed:15192144). 2. Secretory autophagy: IL1B- containing autophagosomes may fuse with endosomes or multivesicular bodies (MVBs) and then merge with the plasma membrane releasing soluble IL1B or IL1B-containing exosomes (PubMed:24201029). However, autophagy impacts IL1B production at several levels and its role in secretion is still controversial 3. Secretion via exosomes: ATP-activation of P2RX7 leads to the formation of MVBs containing exosomes with entrapped IL1B, CASP1 and other inflammasome components. These MVBs undergo exocytosis with the release of exosomes. The release of soluble IL1B occurs after the lysis of exosome membranes (By similarity). 4. Secretion by microvesicle shedding: activation of the ATP receptor P2RX7 may induce an immediate shedding of membrane-derived microvesicles containing IL1B and possibly inflammasome components. The cytokine is then released in the extracellular compartment after microvesicle lysis (PubMed:11728343). 5. Release by translocation through permeabilized plasma membrane. This may occur in cells undergoing pyroptosis due to sustained activation of the inflammasome (By similarity). These mechanisms may not be not mutually exclusive. {ECO:0000250|UniProtKB:P10749, ECO:0000269|PubMed:11728343, ECO:0000269|PubMed:15192144, ECO:0000305|PubMed:24201029}
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(IL1B Antibody (Center)(Ascites). 293 cell lysates transiently transfected with the IL1B gene.)
WB (Western Blot)
(IL1B Antibody (Center)(Ascites). 293 cell lysates transiently transfected with the IL1B gene.)
Related Product Information for anti-IL1B antibody
The protein encoded by this gene is a member of the
interleukin 1 cytokine family. This cytokine is produced by
activated macrophages as a proprotein, which is proteolytically
processed to its active form by caspase 1 (CASP1/ICE). This
cytokine is an important mediator of the inflammatory response, and
is involved in a variety of cellular activities, including cell
proliferation, differentiation, and apoptosis. The induction of
cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central
nervous system (CNS) is found to contribute to inflammatory pain
hypersensitivity. This gene and eight other interleukin 1 family
genes form a cytokine gene cluster on chromosome 2. [provided by
RefSeq].
interleukin 1 cytokine family. This cytokine is produced by
activated macrophages as a proprotein, which is proteolytically
processed to its active form by caspase 1 (CASP1/ICE). This
cytokine is an important mediator of the inflammatory response, and
is involved in a variety of cellular activities, including cell
proliferation, differentiation, and apoptosis. The induction of
cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central
nervous system (CNS) is found to contribute to inflammatory pain
hypersensitivity. This gene and eight other interleukin 1 family
genes form a cytokine gene cluster on chromosome 2. [provided by
RefSeq].
Product Categories/Family for anti-IL1B antibody
References
Lee, B., et al. J. Immunol. 185(10):5926-5934(2010)
Arana-Argaez, V.E., et al. J. Biol. Chem. 285(43):32824-32833(2010)
Zhang, Z., et al. J. Biol. Chem. 285(43):33092-33103(2010)
Wang, D., et al. Nat. Immunol. 11(10):905-911(2010)
Gein, O.N., et al. Patol Fiziol Eksp Ter 1, 10-13 (2010) :
Arana-Argaez, V.E., et al. J. Biol. Chem. 285(43):32824-32833(2010)
Zhang, Z., et al. J. Biol. Chem. 285(43):33092-33103(2010)
Wang, D., et al. Nat. Immunol. 11(10):905-911(2010)
Gein, O.N., et al. Patol Fiziol Eksp Ter 1, 10-13 (2010) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
interleukin-1 beta proprotein
NCBI Official Synonym Full Names
interleukin 1, beta
NCBI Official Symbol
IL1B
NCBI Official Synonym Symbols
IL-1; IL1F2; IL1-BETA
NCBI Protein Information
interleukin-1 beta
UniProt Protein Name
Interleukin-1 beta
UniProt Gene Name
IL1B
UniProt Synonym Gene Names
IL1F2; IL-1 beta
UniProt Entry Name
IL1B_HUMAN
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Product Notes
The IL1B il1b (Catalog #AAA283465) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 148-174. The IL1B Antibody (Center) (Ascites) reacts with Human (Predicted Reactivity: Monkey, Rabbit) and may cross-react with other species as described in the data sheet. AAA Biotech's IL1B can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the IL1B il1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL1B, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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