Mouse anti-Human, Mouse IL25 / IL17E Monoclonal Antibody | anti-IL25 antibody

Mouse Monoclonal [clone 68C1039.2] (IgG1) to Human IL25 / IL17E

Cat. Num. AAA12340

• Gene Names: IL25; IL17E
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Protein G Purified
• Synonyms: IL25 / IL17E, Antibody; Mouse Monoclonal [clone 68C1039.2] (IgG1) to Human IL25 / IL17E; Anti-IL25 / IL17E Antibody (aa115-132, clone 68C1039.2) IHC-plus; IL25; Interleukin 17E; Interleukin 25; Interleukin-25; IL17E; IL-17E; IL-25; Interleukin-17E; Human IL25; anti-IL25 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 68C1039.2
• Specificity: A synthetic peptide of amino acids 115-132, obtained from the human IL-17E protein sequence, was used as immunogen
• Purity/Purification: Protein G Purified
• Form/Format: PBS with 0.05% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 177

• Applicable Applications for anti-IL25 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (10 ug/ml), WB (2-4 ug/ml); Optimal dilution to be determine by the researcher.
• Immunogen Species: IL25 / IL17E antibody was raised against Human
• Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody , followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 10 ug/ml.
• Immunogen Type: Synthetic peptide
• Immunogen: IL25 / IL17E antibody was raised against synthetic peptide from human IL25 / IL-25.
• Epitope: aa115-132
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Short term: Store at 4°C. Long term: aliquot and store at -20C. Avoid freeze/thaw cycles.

IHC (Immunohistochemistry)

(Anti-IL-25 antibody IHC of human adrenal cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Product Categories/Family for anti-IL25 antibody

Family: Interleukin

NCBI and Uniprot Product Information

• NCBI GI #: 12232485
• NCBI GeneID: 64806
• NCBI Accession #: NP_073626.1
• NCBI GenBank Nucleotide #: NM_022789.3
• UniProt Accession #: Q9H293; Q2M3F0; Q8IZV3; Q8WXB0
• NCBI Official Full Name: interleukin-25 isoform 1
• NCBI Official Synonym Full Names: interleukin 25
• NCBI Official Symbol: IL25
• NCBI Official Synonym Symbols: IL17E
• NCBI Protein Information: interleukin-25; interleukin-17E
• UniProt Protein Name: Interleukin-25
• UniProt Gene Name: IL25
• UniProt Synonym Gene Names: IL17E; IL-25; IL-17E
• UniProt Entry Name: IL25_HUMAN