Mouse IL3RA/CD123 Monoclonal Antibody | anti-IL3RA antibody

IL3RA/CD123 (Acute Myeloid Leukemia Marker)

Cat. Num. AAA214765

• Gene Names: IL3RA; IL3R; CD123; IL3RX; IL3RY; IL3RAY; hIL-3Ra
• Reactivity: Human. Others not known.
• Applications: Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: IL3RA/CD123, Antibody; IL3RA/CD123 (Acute Myeloid Leukemia Marker); CD123; hIL3Ra; IL-3R-alpha; IL-3RA; IL3RAX; IL3RAY; IL3RX; IL3RY; Interleukin-3 Receptor, alpha; Interleukin3 receptor; Interleukin3 receptor, Y-chromosomal; anti-IL3RA antibody

• Host: Mouse
• Reactivity: Human. Others not known.
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Clone Number: IL3RA/1531
• Specificity: CD123 is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3. This gene and the gene encoding the colony stimulating factor 2 receptor alpha chain (CSF2RA) form a cytokine receptor gene cluster in a X-Y pseudo-autosomal region on chromosomes X or Y.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 300

• Applicable Applications for anti-IL3RA antibody: IHC (Immunohistochemistry)
• Immunogen: Recombinant fragment of human IL3RA protein (around aa26-171) (exact sequence is proprietary)
• Positive Control: THP-1, RPMI-8226 or HDLM-2 Cells. Tonsil, Lymph Node or Stomach
• Cellular Localization: Cell Surface and Cytoplasmic
• Chromosome Location: Xp22.3 or Yp11.3
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C.; Antibody without azide - store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Application Data

(Analysis of Protein Array containing >19,000 full-length human proteins using CD123 Mouse Monoclonal Antibody (IL3RA/1531). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

SDS_PAGE

(SDS-PAGE Analysis Purified IL3RA/CD123 Mouse Monoclonal Antibody (IL3RA/1531). Confirmation of Purity and Integrity of Antibody.)

IHC (Immunohistochemisry)

(Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with IL3RA/CD123 Mouse Monoclonal Antibody (IL3RA/1531).)

IHC (Immunohiostchemistry)

(Formalin-fixed, paraffin-embedded human Lymph Node stained with IL3RA/CD123 Mouse Monoclonal Antibody (IL3RA/1531).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Tonsil stained with IL3RA/CD123 Mouse Monoclonal Antibody (IL3RA/1531).)

References

Rollins-Raval M, Pillai R, Warita K, Mitsuhashi-Warita T, Mehta R, Boyiadzis M, Djokic M, Kant JA, Roth CG.CD123 immunohistochemical expression in acute myeloid leukemia is associated with underlying FLT3-ITD and NPM1 mutations.Appl Immunohistochem Mol Morphol. 2013 May; 21(3):212-7

NCBI and Uniprot Product Information

• NCBI GI #: 391224440
• NCBI GeneID: 3563
• NCBI Accession #: NP_001254642.1
• NCBI GenBank Nucleotide #: NM_001267713.1
• UniProt Accession #: P26951; Q5HYQ7; Q5HYQ8; Q9UEH7; A8K3F3; B9VI81
• Molecular Weight: ~70kDa
• NCBI Official Full Name: interleukin-3 receptor subunit alpha isoform 2
• NCBI Official Synonym Full Names: interleukin 3 receptor subunit alpha
• NCBI Official Symbol: IL3RA
• NCBI Official Synonym Symbols: IL3R; CD123; IL3RX; IL3RY; IL3RAY; hIL-3Ra
• NCBI Protein Information: interleukin-3 receptor subunit alpha
• UniProt Protein Name: Interleukin-3 receptor subunit alpha
• UniProt Gene Name: IL3RA
• UniProt Synonym Gene Names: IL3R; IL-3 receptor subunit alpha; IL-3R subunit alpha; IL-3R-alpha; IL-3RA