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FCM (Flow Cytometry)
(Flow cytometric analysis of SH-SY-5Y cells with ILF2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Rabbit ILF2 Monoclonal Antibody | anti-ILF2 antibody
ILF2 Antibody
Gene Names
ILF2; NF45; PRO3063
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
ILF2, Antibody; ILF2 Antibody; HGNC:6037 antibody; ILF 2 antibody; ilf2 antibody; ILF2_HUMAN antibody; Interleukin enhancer binding factor 2 antibody; Interleukin enhancer binding factor 2; 45kDa antibody; Interleukin enhancer-binding factor 2 antibody; MGC8391 antibody; NF 45 antibody; NF45 antibody; Nuclear factor of activated T cells 45 kDa antibody; Nuclear factor of activated T-cells 45 kDa antibody; PRO3063 antibody; anti-ILF2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
JU50-33
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-ILF2 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500
IHC: 1:50-1:200
IP: 1:10-1:20
FC/FACS: 1:50-1:100
IHC: 1:50-1:200
IP: 1:10-1:20
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM (Flow Cytometry)
(Flow cytometric analysis of SH-SY-5Y cells with ILF2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
FCM (Flow Cytometry)
(Flow cytometric analysis of SH-SY-5Y cells with ILF2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ILF2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ILF2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ILF2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ILF2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-ILF2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-ILF2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ILF2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ILF2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ILF2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ILF2 antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of ILF2 on different cell lysates using anti-ILF2 antibody at 1/500 dilution. Positive control: Lane 1: Mouse testis tissue Lane 2: HL-60 Lane 3: SH-SY-5Y)
WB (Western Blot)
(Western blot analysis of ILF2 on different cell lysates using anti-ILF2 antibody at 1/500 dilution. Positive control: Lane 1: Mouse testis tissue Lane 2: HL-60 Lane 3: SH-SY-5Y)
Related Product Information for anti-ILF2 antibody
NF45 (ILF2, nuclear factor of activated T cells 45 kDa) is a transcription factor that interacts with NF90 (ILF3, DRBP76) to regulate gene expression. NF45 and NF90 are proteins that belong to the double-stranded RNA-binding protein family and both are substrates for the dsRNA-activated protein kinase, PKR. The NF45/NF90 heterodimer is mainly involved in regulating IL-2 expression by binding to the antigen receptor response element (ARRE) target sequence of the IL-2 enhancer. In neuronal cells, the NF45/NF90 heterodimer can repress human rhinovirus type 2 replication by binding to a 5' untranslated region of the viral RNA that encodes the internal ribosome entry site (IRES).
Product Categories/Family for anti-ILF2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
43,062 Da
NCBI Official Full Name
interleukin enhancer-binding factor 2 isoform 1
NCBI Official Synonym Full Names
interleukin enhancer binding factor 2
NCBI Official Symbol
ILF2
NCBI Official Synonym Symbols
NF45; PRO3063
NCBI Protein Information
interleukin enhancer-binding factor 2; interleukin enhancer binding factor 2, 45kD; nuclear factor of activated T-cells, 45-kDa; interleukin enhancer binding factor 2, 45kDa
UniProt Protein Name
Interleukin enhancer-binding factor 2
UniProt Gene Name
ILF2
UniProt Synonym Gene Names
NF45
UniProt Entry Name
ILF2_HUMAN
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Product Notes
The ILF2 ilf2 (Catalog #AAA30430) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ILF2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ILF2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:500 IHC: 1:50-1:200 IP: 1:10-1:20 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the ILF2 ilf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ILF2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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