Rabbit anti-Human ING5 Monoclonal Antibody | anti-ING5 antibody

Recombinant Anti-ING5 Antibody, Rabbit Monoclonal

Cat. Num. AAA255709

• Gene Names: ING5; p28ING5
• Reactivity: Human
• Applications: Immunoprecipitation, ELISA, Western Blot
• Purity: Protein A
• Synonyms: ING5, Antibody; Recombinant Anti-ING5 Antibody, Rabbit Monoclonal; ING5 Antibody, Rabbit MAb; inhibitor of growth family, member 5; Anti-p28ING5 Antibody; anti-ING5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 119
• Specificity: Human ING5
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-ING5 antibody: IP (Immunoprecipitation), ELISA, WB (Western Blot)
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Human ING5 Protein
• Conjugation: Unconjugated
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human ING5 (rh ING5).
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

WB (Western Blot)

(Anti-ING5 rabbit monoclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:28 kDaObserved band size:34 kDa(We are unsure as to the identity of these extra bands.))

IP (Immunoprecipitation)

(ING5 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell Lysate2 uL anti-ING5 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-ING5 rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 28 kDaObserved band size: 32 kDa)

Related Product Information for anti-ING5 antibody

ING5 belongs to the ING family. It contains 1 PHD-type zinc finger and is a component of the HBO1 complex. HBO1 complex has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. HBO1 complex composed at least of ING4 or ING5, KAT7/HBO1, MEAF6, and one of PHF15, PHF16, and PHF17. ING5 also is a component of the MOZ/MORF complex which is composed at least of ING5, KAT6A, KAT6B, MEAF6, and one of BRPF1, BRD1/BRPF2, and BRPF3. It interacts with EP300 and TP53. MOZ/MORF complex has a histone H3 acetyltransferase activity. Through chromatin acetylation, ING5 may regulate DNA replication and may function as a transcriptional coactivator. It interacts with H3K4me3 and to a lesser extent with H3K4me2. ING5 is similar to ING1, a tumor suppressor protein that can interact with TP53, inhibit cell growth, and induce apoptosis. It can bind TP EP300/p300, a component of the histone acetyltransferase complex, suggesting its involvement in the TP53-dependent regulatory pathway.

References

Zhang F, et al. (2011) The inhibitor of growth protein 5 (ING5) depends on INCA1 as a co-factor for its antiproliferative effects. PLoS One. (7):e21505.Zheng HC, et al. (2011) The nuclear to cytoplasmic shift of ING5 protein during colorectal carcinogenesis with their distinct links to pathologic behaviors of carcinomas. Hum Pathol. 42(3):424-33.Xing YN, et al. (2011) The altered expression of ING5 protein is involved in gastric carcinogenesis and subsequent progression. Hum Pathol. 42(1):25-35.

NCBI and Uniprot Product Information

• NCBI GI #: 57012960
• NCBI GeneID: 84289
• UniProt Accession #: Q8WYH8; Q53NU6; Q57Z54; Q9BS30; A8K1P3
• Molecular Weight: 240
• NCBI Official Full Name: Inhibitor of growth protein 5
• NCBI Official Synonym Full Names: inhibitor of growth family, member 5
• NCBI Official Symbol: ING5
• NCBI Official Synonym Symbols: p28ING5
• NCBI Protein Information: inhibitor of growth protein 5
• UniProt Protein Name: Inhibitor of growth protein 5
• UniProt Gene Name: ING5
• UniProt Entry Name: ING5_HUMAN