WB (Western Blot)
(Detection of mouse iNOS by western blot. Samples: Whole cell lysate (10 ug) from RAW 264.7 and LPS-treated RAW 264.7 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-iNOS recombinant monoclonal antibody (AAA213630 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
iNOS recombinant antibody
Rabbit anti-iNOS Recombinant Monoclonal Antibody [BLR181J]
Gene Names
Nos2; iNOS; Nos-2; Nos2a; i-NOS; NOS-II
Reactivity
Mouse
Applications
Immunocytochemistry, Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
iNOS, Recombinant Antibody; Rabbit anti-iNOS Recombinant Monoclonal Antibody [BLR181J]; nitric oxide synthase 2, inducible, macrophage; Nos-2; peptidyl-cysteine S-nitrosylase NOS2; Nos2a; NOS type II; nitric oxide synthase, inducible; MAC-NOS; i-NOS; iNOS; inducible nitric oxide synthase; inducible NOS; NOS-II; inducible NO synthase; macrophage NOS; Nitric oxide synthase, inducible; nitric oxide synthase 2, inducible; iNOS recombinant antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Monoclonal
Clone Number
BLR181J
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
Concentration
1000ug/ml (varies by lot)
Applicable Applications for iNOS recombinant antibody
ICC (Immunocytochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1094 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of mouse iNOS by western blot. Samples: Whole cell lysate (10 ug) from RAW 264.7 and LPS-treated RAW 264.7 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-iNOS recombinant monoclonal antibody (AAA213630 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot)
(Detection of mouse iNOS by western blot. Samples: Whole cell lysate (10 ug) from RAW 264.7 and LPS-treated RAW 264.7 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-iNOS recombinant monoclonal antibody (AAA213630 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
IP (Immunoprecipitation)
(Detection of mouse iNOS by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from LPS treated RAW 264.7 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-iNOS recombinant monoclonal antibody (AAA213630 lot 1) used for IP at 6 ul/mg lysate. iNOS was also immunoprecipitated by a second antibody against a different epitope of iNOS (Antibody 2). For blotting immunoprecipitated iNOS, AAA213630 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of mouse iNOS by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from LPS treated RAW 264.7 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-iNOS recombinant monoclonal antibody (AAA213630 lot 1) used for IP at 6 ul/mg lysate. iNOS was also immunoprecipitated by a second antibody against a different epitope of iNOS (Antibody 2). For blotting immunoprecipitated iNOS, AAA213630 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
ICC (Immunocytochemistry)
(Detection of mouse iNOS in RAW264.7 cells treated with LPS (left) and untreated (right) by ICC. Antibody: Rabbit anti-iNOS recombinant monoclonal antibody (AAA213630 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of mouse iNOS in RAW264.7 cells treated with LPS (left) and untreated (right) by ICC. Antibody: Rabbit anti-iNOS recombinant monoclonal antibody (AAA213630 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .Substrate: DAB.)
Related Product Information for iNOS recombinant antibody
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide synthase is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. [taken from NCBI Entrez Gene (Gene ID: 4843)].
Product Categories/Family for iNOS recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
130,575 Da
NCBI Official Full Name
nitric oxide synthase, inducible
NCBI Official Synonym Full Names
nitric oxide synthase 2, inducible
NCBI Official Symbol
Nos2
NCBI Official Synonym Symbols
iNOS; Nos-2; Nos2a; i-NOS; NOS-II
NCBI Protein Information
nitric oxide synthase, inducible; MAC-NOS; NOS type II; inducible NO synthase; inducible NOS; inducible nitric oxide synthase; macrophage NOS; nitric oxide synthase 2, inducible, macrophage; peptidyl-cysteine S-nitrosylase NOS2
UniProt Protein Name
Nitric oxide synthase, inducible
UniProt Gene Name
Nos2
UniProt Synonym Gene Names
Inosl; Inducible NOS; iNOS; MAC-NOS
UniProt Entry Name
NOS2_MOUSE
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Product Notes
The iNOS nos2 (Catalog #AAA213630) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-iNOS Recombinant Monoclonal Antibody [BLR181J] reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's iNOS can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the iNOS nos2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "iNOS, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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