WB (Western Blot)
(Detection of human iNOS by western blot. Samples: Whole cell lysate (10 ug) from DLD-1 and DLD-1 induced (IFN-gamma (56 ng/mL), IL-1beta (145 pg/mL), and TNFa (10 ng/mL)) prepared using NETN lysis buffer. Antibody: Rabbit anti-iNOS recombinant monoclonal antibody (AAA213633 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
iNOS recombinant antibody
Rabbit anti-iNOS Recombinant Monoclonal Antibody [BLR188J]
Gene Names
NOS2; NOS; INOS; NOS2A; HEP-NOS
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
iNOS, Recombinant Antibody; Rabbit anti-iNOS Recombinant Monoclonal Antibody [BLR188J]; nitric oxide synthase, macrophage; HEP-NOS; peptidyl-cysteine S-nitrosylase NOS2; NOS2A; NOS, type II; NOS type II; NOS; nitric oxide synthase 2A (inducible, hepatocytes); nitric oxide synthase 2, inducible; INOS; hepatocyte NOS; inducible NO synthase; inducible NOS; nitric oxide synthase, inducible; Nitric oxide synthase, inducible; nitric oxide synthase 2; iNOS recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BLR188J
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
100ug/ml (varies by lot)
Applicable Applications for iNOS recombinant antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1103 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human iNOS by western blot. Samples: Whole cell lysate (10 ug) from DLD-1 and DLD-1 induced (IFN-gamma (56 ng/mL), IL-1beta (145 pg/mL), and TNFa (10 ng/mL)) prepared using NETN lysis buffer. Antibody: Rabbit anti-iNOS recombinant monoclonal antibody (AAA213633 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot)
(Detection of human iNOS by western blot. Samples: Whole cell lysate (10 ug) from DLD-1 and DLD-1 induced (IFN-gamma (56 ng/mL), IL-1beta (145 pg/mL), and TNFa (10 ng/mL)) prepared using NETN lysis buffer. Antibody: Rabbit anti-iNOS recombinant monoclonal antibody (AAA213633 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
IP (Immunoprecipitation)
(Detection of human iNOS by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from induced DLD-1 cells (hIFN-gamma (56 ng/mL), hIL-1beta (145 pg/mL), and hTNFa (10 ng/mL)) prepared using NETN lysis buffer. Antibodies: Rabbit anti-iNOS recombinant monoclonal antibody (AAA213633 lot 1) used for IP at 20 ul/mg lysate. iNOS was also immunoprecipitated by a second antibody against a different epitope of iNOS (BL-2053B-2F8). For blotting immunoprecipitated iNOS, AAA213633 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human iNOS by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from induced DLD-1 cells (hIFN-gamma (56 ng/mL), hIL-1beta (145 pg/mL), and hTNFa (10 ng/mL)) prepared using NETN lysis buffer. Antibodies: Rabbit anti-iNOS recombinant monoclonal antibody (AAA213633 lot 1) used for IP at 20 ul/mg lysate. iNOS was also immunoprecipitated by a second antibody against a different epitope of iNOS (BL-2053B-2F8). For blotting immunoprecipitated iNOS, AAA213633 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for iNOS recombinant antibody
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide synthase is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. [taken from NCBI Entrez Gene (Gene ID: 4843)].
Product Categories/Family for iNOS recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
131,117 Da
NCBI Official Full Name
nitric oxide synthase, inducible
NCBI Official Synonym Full Names
nitric oxide synthase 2, inducible
NCBI Official Symbol
NOS2
NCBI Official Synonym Symbols
NOS; INOS; NOS2A; HEP-NOS
NCBI Protein Information
nitric oxide synthase, inducible; NOS type II; NOS, type II; inducible NOS; hepatocyte NOS; inducible NO synthase; nitric oxide synthase, macrophage; peptidyl-cysteine S-nitrosylase NOS2; nitric oxide synthase 2A (inducible, hepatocytes)
UniProt Protein Name
Nitric oxide synthase, inducible
UniProt Gene Name
NOS2
UniProt Synonym Gene Names
NOS2A; HEP-NOS; Inducible NOS; iNOS
UniProt Entry Name
NOS2_HUMAN
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Product Notes
The iNOS nos2 (Catalog #AAA213633) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-iNOS Recombinant Monoclonal Antibody [BLR188J] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's iNOS can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the iNOS nos2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "iNOS, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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