IP (Immunoprecipitation)
(Immunoprecipitating SHIP in Raji whole cell lysateLane 1: Rabbit control IgG instead of in Raji whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: Raji whole cell lysate?500ug?Lane 3: Raji whole cell lysate (10ug))
INPP5D recombinant antibody
INPP5D Antibody
Gene Names
INPP5D; SHIP; SHIP1; SHIP-1; hp51CN; SIP-145
Reactivity
Human
Applications
Immunoprecipitation, Immunohistochemistry, Western Blot, ELISA
Purity
Affinity-chromatography
Synonyms
INPP5D, Recombinant Antibody; INPP5D Antibody; Phosphatidylinositol 3;4;5-trisphosphate 5-phosphatase 1 (EC 3.1.3.86) (Inositol polyphosphate-5-phosphatase of 145 kDa) (SIP-145) (SH2 domain-containing inositol 5'-phosphatase 1) (SH2 domain-containing inositol phosphatase 1) (SHIP-1) (p150Ship) (hp51CN); INPP5D; SHIP SHIP1; INPP5D recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
9G11
Purity/Purification
Affinity-chromatography
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, PH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applicable Applications for INPP5D recombinant antibody
IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot), ELISA
Antibody Type
Recombinant Antibody
Conjugation
Non-conjugated
Immunogen
A synthesized peptide derived from human SHIP
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
IP (Immunoprecipitation)
(Immunoprecipitating SHIP in Raji whole cell lysateLane 1: Rabbit control IgG instead of in Raji whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: Raji whole cell lysate?500ug?Lane 3: Raji whole cell lysate (10ug))
IP (Immunoprecipitation)
(Immunoprecipitating SHIP in Raji whole cell lysateLane 1: Rabbit control IgG instead of in Raji whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: Raji whole cell lysate?500ug?Lane 3: Raji whole cell lysate (10ug))
IHC (Immunohiostchemistry)
(IHC image diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
IHC (Immunohiostchemistry)
(IHC image diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
WB (Western Blot)
(Western BlotPositive WB detected in: Raji whole cell lysate, K562 whole cell lysateAll lanes: INPP5D antibody at 1:1500SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 134, 110 kDaObserved band size: 145 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in: Raji whole cell lysate, K562 whole cell lysateAll lanes: INPP5D antibody at 1:1500SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 134, 110 kDaObserved band size: 145 kDa)
Related Product Information for INPP5D recombinant antibody
Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways. Acts as a negative regulator of B-cell antigen receptor signaling. Mediates signaling from the FC-gamma-RIIB receptor (FCGR2B), playing a central role in terminating signal transduction from activating immune/hematopoietic cell receptor systems. Acts as a negative regulator of myeloid cell proliferation/survival and chemotaxis, mast cell degranulation, immune cells homeostasis, integrin alpha-IIb/beta-3 signaling in platelets and JNK signaling in B-cells. Regulates proliferation of osteoclast precursors, macrophage programming, phagocytosis and activation and is required for endotoxin tolerance. Involved in the control of cell-cell junctions, CD32a signaling in neutrophils and modulation of EGF-induced phospholipase C activity. Key regulator of neutrophil migration, by governing the formation of the leading edge and polarization required for chemotaxis. Modulates FCGR3/CD16-mediated cytotoxicity in NK cells. Mediates the activin/TGF-beta-induced apoptosis through its Smad-dependent expression. May also hydrolyze PtdIns(1,3,4,5)P4, and could thus affect the levels of the higher inositol polyphosphates like InsP6.
Product Categories/Family for INPP5D recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
133,292 Da
NCBI Official Full Name
phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1 isoform a
NCBI Official Synonym Full Names
inositol polyphosphate-5-phosphatase, 145kDa
NCBI Official Symbol
INPP5D
NCBI Official Synonym Symbols
SHIP; SHIP1; SHIP-1; hp51CN; SIP-145
NCBI Protein Information
phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1; p150Ship; SH2 domain-containing inositol phosphatase 1; SH2 containing inositol phosphatase, isoform b; SH2 domain-containing inositol 5'-phosphatase 1; SH2 domain-containing inositol-5'-phosphatas
UniProt Protein Name
Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1
UniProt Gene Name
INPP5D
UniProt Synonym Gene Names
SHIP; SHIP1; SIP-145; SH2 domain-containing inositol phosphatase 1; SHIP-1; hp51CN
UniProt Entry Name
SHIP1_HUMAN
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Product Notes
The INPP5D inpp5d (Catalog #AAA243808) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The INPP5D Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's INPP5D can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the INPP5D inpp5d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "INPP5D, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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