Rabbit anti-Human Insulin Monoclonal Antibody | anti-INS antibody

Insulin Rabbit mAb

Cat. Num. AAA282668

• Reactivity: Human
• Applications: ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Affinity purification
• Synonyms: Insulin, Antibody; Insulin Rabbit mAb; IDDM; ILPR; IRDN; IDDM1; IDDM2; PNDM4; MODY10; Insulin; anti-INS antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
• Sequence: MALWMRLLPLLALLALWGPDPAAAFVNQHLCGSHLVEALYLVCGERGFFYTPKTRREAEDLQVGQVELGGGPGAGSLQPLALEGSLQKRGIVEQCCTSIC

• Applicable Applications for anti-INS antibody: ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
• Cross Reactivity: Human, Mouse, Rat
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Insulin (P01308).
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

ICC (Immunocytochemistry)

(Confocal imaging of human pancreas using Insulin Rabbit mAb (AAA282668,at dilution of 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 40x. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.)

IHC (Immunohistochemisry)

(Immunohistochemistry analysis of paraffin-embedded Mouse pancreatic islet using Insulin Rabbit mAb (AAA282668) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.)

IHC (Immunohiostchemistry)

(Immunohistochemistry analysis of paraffin-embedded Rat pancreatic islet using Insulin Rabbit mAb (AAA282668) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.)

WB (Western Blot)

(Western blot analysis of various lysates using Insulin Rabbit mAb (AAA282668) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 3min.)

Related Product Information for anti-INS antibody

This gene encodes insulin, a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified, including insulin-dependent diabetes mellitus, permanent neonatal diabetes diabetes mellitus, maturity-onset diabetes of the young type 10 and hyperproinsulinemia. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region.

Product Categories/Family for anti-INS antibody

Cancer; Signal Transduction; Cell Biology Developmental Biology; Growth factors; Endocrine Metabolism; AMPK Signaling Pathway; Endocrine and metabolic diseases; Diabetes; Obesity; Immunology Inflammation; NF-kB Signaling Pathway; Neuroscience; Stem Cells;

NCBI and Uniprot Product Information

• NCBI GI #: 386828
• NCBI GeneID: 3630
• UniProt Accession #: P01308; Q5EEX2
• Molecular Weight: Calculated MW: 12kDa; Observed MW: 12kDa
• UniProt Protein Name: Insulin
• UniProt Gene Name: INS
• UniProt Entry Name: INS_HUMAN