WB (Western Blot)
(Western blot analysis of various lysates using Integrin alpha 1/CD49a Rabbit PolymAb® (AAA283086) at 1:25000 dilution incubated overnight at 4?.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 20s.)
Rabbit anti-Human, Mouse Integrin alpha 1/CD49a Monoclonal Antibody | anti-ITGA1 antibody
Integrin alpha 1/CD49a Rabbit PolymAb
Reactivity
Human, Mouse
Applications
ELISA, Western Blot
Purity
Affinity purification
Synonyms
Integrin alpha 1/CD49a, Antibody; Integrin alpha 1/CD49a Rabbit PolymAb; VLA1; CD49a; anti-ITGA1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Sequence
FNVDVKNSMTFSGPVEDMFGYTVQQYENEEGKWVLIGSPLVGQPKNRTGDVYKCPVGRGESLPCVKLDLPVNTSIPNVTEVKENMTFGSTLVTNPNGGFLACGPLYAYRCGHLHYTTGICSDVSPTFQVVNSIAPVQECSTQLDIVIVLDGSNSIYPWDSVTAFLNDLLERMDIGPKQTQVGIVQYGENVTHEFNLNKYSSTEEVLVAAKKIVQRGGRQTMTALGIDTARKEAFTEARGARRGVKKVMVIVTDGESHDNHRLKKVIQDCEDENIQRFSIAILGSYNRGNLSTEKFVEEIKSIASEPTEKHFFNVSDELALVTIVKTLGERIFALEATADQSAASFEMEMSQTGFSAHYSQDWVMLGAVGAYDWNGTVVMQKASQIIIPRNTTFNVESTKKNEPLASYLGYTVNSATASSGDVLYIAGQPRYNHTGQVIIYRMEDGNIKILQTLSGEQIGSYFGSILTTTDIDKDSNTDILLVGAPMYMGTEKEEQGKVYVYALNQTRFEYQMSLEPIKQTCCSSRQHNSCTTENKNEPCGARFGTAIAAVKDLNLDGFNDIVIGAPLEDDHGGAVYIYHGSGKTIRKEYAQRIPSGGDGKTLKFFGQSIHGEMDLNGDGLTDVTIGGLGGAALFWSRDVAVVKVTMNFEPNKVNIQKKNCHMEGKETVCINATVCFDVKLKSKEDTIYEADLQYRVTLDSLRQISRSFFSGTQERKVQRNITVRKSECTKHSFYMLDKHDFQDSVRITLDFNLTDPENGPVLDDSLPNSVHEYIPFAKDCGNKEKCISDLSLHVATTEKDLLIVRSQNDKFNVSLTVKNTKDSAYNTRTIVHYSPNLVFSGIEAIQKDSCESNHNITCKVGYPFLRRGEMVTFKILFQFNTSYLMENVTIYLSATSDSEEPPETLSDNVVNISIPVKYEVGLQFYSSASEYHISIAANETVPEVINSTEDIGNEINIFYLIRKSGSFPMPELKLSISFPNMTSNGYPVLYPTGLSSSENANCRPHIFEDPFSINSGKKMTTSTDHLKRGTILDCNTCKFATITCNLTSSDISQVNVSLILWKPTFIKSYFSSLNLTIRGELRSENASLVLSSSNQKRELAIQISKDGLPGRVP
Applicable Applications for anti-ITGA1 antibody
ELISA, WB (Western Blot)
Cross Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 29-1141 of human/mouse Integrin alpha 1/CD49a(NP_852478.1).
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
WB (Western Blot)
(Western blot analysis of various lysates using Integrin alpha 1/CD49a Rabbit PolymAb® (AAA283086) at 1:25000 dilution incubated overnight at 4?.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 20s.)
WB (Western Blot)
(Western blot analysis of various lysates using Integrin alpha 1/CD49a Rabbit PolymAb® (AAA283086) at 1:25000 dilution incubated overnight at 4?.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 20s.)
WB (Western Blot)
(Western blot analysis of various lysates using Integrin alpha 1/CD49a Rabbit PolymAb® (AAA283086) at 1:25000 dilution incubated overnight at 4?.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.)
WB (Western Blot)
(Western blot analysis of various lysates using Integrin alpha 1/CD49a Rabbit PolymAb® (AAA283086) at 1:25000 dilution incubated overnight at 4?.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.)
Related Product Information for anti-ITGA1 antibody
This gene encodes the alpha 1 subunit of integrin receptors. This protein heterodimerizes with the beta 1 subunit to form a cell-surface receptor for collagen and laminin. The heterodimeric receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. The alpha 1 subunit contains an inserted (I) von Willebrand factor type I domain which is thought to be involved in collagen binding.
Product Categories/Family for anti-ITGA1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
Calculated MW: 131kDa
Observed MW: 200kDa
Observed MW: 200kDa
UniProt Protein Name
Integrin alpha-1
UniProt Gene Name
ITGA1
UniProt Entry Name
ITA1_HUMAN
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Product Notes
The ITGA1 itga1 (Catalog #AAA283086) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Integrin alpha 1/CD49a Rabbit PolymAb reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Integrin alpha 1/CD49a can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ITGA1 itga1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: FNVDVKNSMT FSGPVEDMFG YTVQQYENEE GKWVLIGSPL VGQPKNRTGD VYKCPVGRGE SLPCVKLDLP VNTSIPNVTE VKENMTFGST LVTNPNGGFL ACGPLYAYRC GHLHYTTGIC SDVSPTFQVV NSIAPVQECS TQLDIVIVLD GSNSIYPWDS VTAFLNDLLE RMDIGPKQTQ VGIVQYGENV THEFNLNKYS STEEVLVAAK KIVQRGGRQT MTALGIDTAR KEAFTEARGA RRGVKKVMVI VTDGESHDNH RLKKVIQDCE DENIQRFSIA ILGSYNRGNL STEKFVEEIK SIASEPTEKH FFNVSDELAL VTIVKTLGER IFALEATADQ SAASFEMEMS QTGFSAHYSQ DWVMLGAVGA YDWNGTVVMQ KASQIIIPRN TTFNVESTKK NEPLASYLGY TVNSATASSG DVLYIAGQPR YNHTGQVIIY RMEDGNIKIL QTLSGEQIGS YFGSILTTTD IDKDSNTDIL LVGAPMYMGT EKEEQGKVYV YALNQTRFEY QMSLEPIKQT CCSSRQHNSC TTENKNEPCG ARFGTAIAAV KDLNLDGFND IVIGAPLEDD HGGAVYIYHG SGKTIRKEYA QRIPSGGDGK TLKFFGQSIH GEMDLNGDGL TDVTIGGLGG AALFWSRDVA VVKVTMNFEP NKVNIQKKNC HMEGKETVCI NATVCFDVKL KSKEDTIYEA DLQYRVTLDS LRQISRSFFS GTQERKVQRN ITVRKSECTK HSFYMLDKHD FQDSVRITLD FNLTDPENGP VLDDSLPNSV HEYIPFAKDC GNKEKCISDL SLHVATTEKD LLIVRSQNDK FNVSLTVKNT KDSAYNTRTI VHYSPNLVFS GIEAIQKDSC ESNHNITCKV GYPFLRRGEM VTFKILFQFN TSYLMENVTI YLSATSDSEE PPETLSDNVV NISIPVKYEV GLQFYSSASE YHISIAANET VPEVINSTED IGNEINIFYL IRKSGSFPMP ELKLSISFPN MTSNGYPVLY PTGLSSSENA NCRPHIFEDP FSINSGKKMT TSTDHLKRGT ILDCNTCKFA TITCNLTSSD ISQVNVSLIL WKPTFIKSYF SSLNLTIRGE LRSENASLVL SSSNQKRELA IQISKDGLPG RVP. It is sometimes possible for the material contained within the vial of "Integrin alpha 1/CD49a, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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