Application Data
(Fig.2: Negative control: only secondary antibody applied.)
Mouse Integrin beta 1D Monoclonal Antibody | anti-ITGB1 antibody
Mouse anti Integrin beta 1D
Gene Names
ITGB1; CD29; FNRB; MDF2; VLAB; GPIIA; MSK12; VLA-BETA
Reactivity
Canine, Human, Mouse, Swine
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry
Synonyms
Integrin beta 1D, Antibody; Mouse anti Integrin beta 1D; anti-ITGB1 antibody
Host
Mouse
Reactivity
Canine, Human, Mouse, Swine
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2B1
Specificity
2B1 recognizes specifically the cytoplasmic domain of integrin subunit beta1D present in cardiac and skeletal muscle.
Form/Format
Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
Applicable Applications for anti-ITGB1 antibody
WB (Western Blot), IHC (Immunohistochemistry), ICC (Immunocytochemistry)
Source Note
2B1 is a Mouse monoclonal IgG1, kappa antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a Mouse immunized with a synthetic peptide corresponding to the C-terminal 24 amino acids of integrin beta1D including an appending N-terminal cysteine (CQENPIYKS-PINNFKNPNYGRKAGL) coupled to keyhole limpet hemocyanin.
Reactivity Note
A broad species reactivity is expected because of the conserved nature of the epitope.
Preparation and Storage
Store at 4 degree C, or in small aliquots at -20 degree C.
Application Data
(Fig.2: Negative control: only secondary antibody applied.)
Application Data
(Fig.2: Negative control: only secondary antibody applied.)
Application Data
(Fig.1: Frozen sections of porcine myocardium immunostained for integrin β1D using a 1:200 dillution of 2B1)
Application Data
(Fig.1: Frozen sections of porcine myocardium immunostained for integrin β1D using a 1:200 dillution of 2B1)
Related Product Information for anti-ITGB1 antibody
Integrins are a family of heterodimeric membrane glycoproteins consisting of non-covalently associated alpha and beta subunits. More than 18 alpha and 8 beta subunits with numerous splice variant isoforms have been identified in mammals. In general, integrins function as receptors for extracellular matrix proteins. Certain integrins can also bind to soluble ligands or to counter-receptors on adjacent cells, such as the intracellular adhesion molecules (ICAMs), resulting in aggregation of cells. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migRation and apoptosis. There are two major forms of integrin beta1: beta1A and beta1D, which differ in 13 amino acids. Their distribution pattern in adult tissues is mutually exclusive. beta1A is present in all tissues, except cardiac and skeletal muscle, which instead express the beta1D variant.
Product Categories/Family for anti-ITGB1 antibody
References
van der Flier, A., Gaspar, A. C., Thorsteinsdottir, S., Baudoin, C., Groeneveld, E., Mummery, C. L., and Sonnenberg, A. (1997). Spatial and temporal expression of the beta1D integrin during Mouse development, Dev Dyn 210, 472-86.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
88,415 Da
NCBI Official Full Name
integrin beta-1 isoform 1D
NCBI Official Synonym Full Names
integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
NCBI Official Symbol
ITGB1
NCBI Official Synonym Symbols
CD29; FNRB; MDF2; VLAB; GPIIA; MSK12; VLA-BETA
NCBI Protein Information
integrin beta-1; glycoprotein IIa; integrin VLA-4 beta subunit; very late activation protein, beta polypeptide
UniProt Protein Name
Integrin beta-1
UniProt Gene Name
ITGB1
UniProt Synonym Gene Names
FNRB; MDF2; MSK12; GPIIA
UniProt Entry Name
ITB1_HUMAN
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Product Notes
The ITGB1 itgb1 (Catalog #AAA77493) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti Integrin beta 1D reacts with Canine, Human, Mouse, Swine and may cross-react with other species as described in the data sheet. AAA Biotech's Integrin beta 1D can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), ICC (Immunocytochemistry). Researchers should empirically determine the suitability of the ITGB1 itgb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Integrin beta 1D, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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