ICC (Immunocytochemistry)
(Immunocytochemical labeling of Integrin beta1 in paraformaldehyde fixed human A431 cells. The cells were labeled with mouse monoclonal anti-Integrin beta1 (IM0321). The antibody was detected using goat anti-mouse Ig DyLight 594.)
Mouse anti-Human Integrin beta1 Monoclonal Antibody | anti-ITGB1 antibody
Integrin beta1 (Extracellular region) M032 Antibody
Gene Names
ITGB1; CD29; FNRB; MDF2; VLAB; GPIIA; MSK12; VLA-BETA
Reactivity
Human
Applications
Immunocytochemistry, ELISA, Western Blot
Purity
Protein G chromatography
Synonyms
Integrin beta1, Antibody; Integrin beta1 (Extracellular region) M032 Antibody; Integrin beta1 (Extracellular region); Integrin b1 M032; anti-ITGB1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
M032
Specificity
Clone M032 detects a 120-150kDa* band corresponding to Integrin beta1 on SDS-PAGE immunoblots of "Native" human A431, A549, MDA-MB-231, and LNCaP cell lysates, as well as human breast, skin, and lung tissues. The antibody weakly detects the denatured form of Integrin beta1. Clone M032 can be used in western blot, immunocytochemistry, and ELISA.
Purity/Purification
Protein G chromatography
Form/Format
Mouse monoclonal antibody purified with protein G chromatography is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-ITGB1 antibody
ICC (Immunocytochemistry), ELISA, WB (Western Blot)
Immunogen
Clone M032 was generated from a proprietary antigen related to the extracellular region of human integrin beta1 expressed in A431 epidermoid carcinoma cell line.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
ICC (Immunocytochemistry)
(Immunocytochemical labeling of Integrin beta1 in paraformaldehyde fixed human A431 cells. The cells were labeled with mouse monoclonal anti-Integrin beta1 (IM0321). The antibody was detected using goat anti-mouse Ig DyLight 594.)
ICC (Immunocytochemistry)
(Immunocytochemical labeling of Integrin beta1 in paraformaldehyde fixed human A431 cells. The cells were labeled with mouse monoclonal anti-Integrin beta1 (IM0321). The antibody was detected using goat anti-mouse Ig DyLight 594.)
WB (Western Blot)
(Western blot analysis of integrin beta1 expression in native human cells and tissues: A549 (lane 1) LNCaP (lane 2), MDA-MB-231 (lane 3) breast (lane 4), lung (lane 5), and skin (lane 6). The blot was probed with mouse monoclonal anti-Integrin beta1 (IM0321) at 1:1000 dilution.)
WB (Western Blot)
(Western blot analysis of integrin beta1 expression in native human cells and tissues: A549 (lane 1) LNCaP (lane 2), MDA-MB-231 (lane 3) breast (lane 4), lung (lane 5), and skin (lane 6). The blot was probed with mouse monoclonal anti-Integrin beta1 (IM0321) at 1:1000 dilution.)
Related Product Information for anti-ITGB1 antibody
Integrins are cell adhesion molecules that can mediate bidirectional transfer of signals across the plasma membrane. The cytoplasmic domains of integrin family members interact with components of the signal transduction apparatus within cells. Integrin receptors contain noncovalently associated alpha and beta subunits that consist of a large extracellular region (the ligand-binding domain), a short transmembrane region, and a cytoplasmic domain of varying length. In mammals, at least 17 alpha subunits and 8 beta subunits have been identified and these proteins can heterodimerize to form at least 22 different receptors. The beta1 subfamily includes 12 distinct integrin proteins that bind to different extracellular matrix molecules. Integrin beta1 has been implicated in various activities including embryonic development, blood vessel, skin, bone, and muscle formation, as well as tumor metastasis and angiogenesis.
References
Iwamoto, D. & Calderwood, D. (2015) Cur Opin Cel Bio. 36:41.
Wang, L. et al. (2012) J Cell Physiol. 227(2):474.
Wang, L. et al. (2012) J Cell Physiol. 227(2):474.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
88,415 Da
NCBI Official Full Name
integrin beta-1 isoform 1A
NCBI Official Synonym Full Names
integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
NCBI Official Symbol
ITGB1
NCBI Official Synonym Symbols
CD29; FNRB; MDF2; VLAB; GPIIA; MSK12; VLA-BETA
NCBI Protein Information
integrin beta-1; glycoprotein IIa; integrin VLA-4 beta subunit; very late activation protein, beta polypeptide
UniProt Protein Name
Integrin beta-1
UniProt Gene Name
ITGB1
UniProt Synonym Gene Names
FNRB; MDF2; MSK12; GPIIA
UniProt Entry Name
ITB1_HUMAN
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Product Notes
The ITGB1 itgb1 (Catalog #AAA71650) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Integrin beta1 (Extracellular region) M032 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Integrin beta1 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ITGB1 itgb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Integrin beta1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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