IHC (Immunohistochemistry)
(DAB staining on IHC-P;Samples: RabbitCardiac Muscle Tissue;Primary Ab: 40ug/ml Mouse Anti-Rabbit IL6 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: ))
Mouse anti-Rabbit, Rat Interleukin 6 Monoclonal Antibody | anti-IL6 antibody
Interleukin 6 (IL6) Monoclonal Antibody
I mmunohistochemistry: 5-40ug/mL
I mmunocytochemistry: 5-40ug/mL
Optimal working dilutions must be determined by end user.
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Samples: RabbitCardiac Muscle Tissue;Primary Ab: 40ug/ml Mouse Anti-Rabbit IL6 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: ))
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Samples: RabbitCardiac Muscle Tissue;Primary Ab: 40ug/ml Mouse Anti-Rabbit IL6 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: ))
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Samples: Rabbit Colon Tissue;Primary Ab: 40ug/ml Mouse Anti-Rabbit IL6 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Samples: Rabbit Colon Tissue;Primary Ab: 40ug/ml Mouse Anti-Rabbit IL6 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
WB (Western Blot)
(Western Blot;Sample: Mouse Spleen ysate;Primary Ab: 5ug/ml Mouse Anti-Rabbit IL6 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
WB (Western Blot)
(Western Blot;Sample: Mouse Spleen ysate;Primary Ab: 5ug/ml Mouse Anti-Rabbit IL6 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
WB (Western Blot)
(Western Blot:Sample: Recombinant IL6, Rabbit.)
WB (Western Blot)
(Western Blot:Sample: Recombinant IL6, Rabbit.)
WB (Western Blot)
(Western Blot; Sample: Rat Spleen lysatePrimary Ab: 1ug/ml Mouse Anti-Rabbit IL6 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: ))
WB (Western Blot)
(Western Blot; Sample: Rat Spleen lysatePrimary Ab: 1ug/ml Mouse Anti-Rabbit IL6 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: ))
NCBI and Uniprot Product Information
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Product Notes
The IL6 il6 (Catalog #AAA21003) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Interleukin 6 (IL6) Monoclonal Antibody reacts with Rabbit, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Interleukin 6 can be used in a range of immunoassay formats including, but not limited to, WB (Westen Blot), IHC (Immunohistochemistry), ICC (Immunocytochemistry), IP (Immunoprecipitation). Western blotting: 0.01-2ug/mL I mmunohistochemistry: 5-40ug/mL I mmunocytochemistry: 5-40ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the IL6 il6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Interleukin 6, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Frequently Asked Questions
What applications is Mouse IL-6 recombinant protein used for?
Mouse IL-6 is primarily used for cell culture differentiation studies, functional assays, and bioassays. Applications include T cell differentiation, hematopoietic progenitor cell induction in semi-solid medium, hybridoma preparation, and TH17 cell differentiation. It stimulates cell proliferation in various cell types, including lymphocytes and hepatocytes.
What is the recommended working concentration for IL-6 in cell assays?
For cell proliferation assays, IL-6 typically shows bioactivity at concentrations below 0.02 ng/mL, with ED50 values ranging from 0.2-1 ng/mL depending on the assay system. For ELISA standards, carrier protein concentrations of 5-10 mg/mL are recommended, while for biological assays, use 0.5-1.0 mg/mL.
How should Mouse IL-6 protein be reconstituted?
Reconstitute lyophilized IL-6 in deionized sterile water at 0.1-1.0 mg/mL concentration. Add 5-50% glycerol (typically 50% final concentration) as a cryoprotectant and aliquot for storage. Alternatively, dilute in sterile neutral buffer containing 0.5-10 mg/mL carrier protein (human or bovine serum albumin) before aliquoting.
What storage conditions are required for stability?
Store lyophilized IL-6 at -20°C or -80°C for up to 12 months. For liquid form, maintain at -20°C to -80°C for 6 months stability. After reconstitution, store aliquots at -80°C to prevent activity loss. Avoid repeated freeze-thaw cycles. Short-term working aliquots can be kept at 4°C for up to one week.
Is the recombinant IL-6 tested for bioactivity and purity?
Yes. Mouse IL-6 from AAA Biotech is >95% pure (verified by SDS-PAGE), bioactive, and typically has endotoxin levels ≤0.1-1 EU/μg. Bioactivity is confirmed through cell proliferation assays using indicator cell lines like 7TD1 or M-NSF-60 cells.
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