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FCM (Flow Cytometry)
(Flow cytometric analysis of Jurkat cells with IRAK2 antibody at 1/50 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Rabbit anti-Human IRAK2 Monoclonal Antibody | anti-IRAK2 antibody
IRAK2 Antibody
Gene Names
IRAK2; IRAK-2
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation
Purity
ProA affinity purified
Synonyms
IRAK2; Monoclonal Antibody; IRAK2 Antibody; il1rak2 antibody; Interleukin 1 receptor associated kinase 2 antibody; Interleukin 1 receptor associated kinase like 2 antibody; Interleukin-1 receptor-associated kinase-like 2 antibody; IRAK 2 antibody; IRAK-2 antibody; Irak2 antibody; IRAK2_HUMAN antibody; MGC150550 antibody; anti-IRAK2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
JG36-23
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-IRAK2 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF),Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:10-1:50
FC/FACS: 1:50-1:100
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:10-1:50
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein within human IRAK2 aa 1-200.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM (Flow Cytometry)
(Flow cytometric analysis of Jurkat cells with IRAK2 antibody at 1/50 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
FCM (Flow Cytometry)
(Flow cytometric analysis of Jurkat cells with IRAK2 antibody at 1/50 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry)
(ICC staining IRAK2 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining IRAK2 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human heart tissue using anti-IRAK2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human heart tissue using anti-IRAK2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-IRAK2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-IRAK2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IRAK2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IRAK2 antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of IRAK2 on different cell lysates using anti-IRAK2 at 1/500 dilution. Positive control�� Line 1: PC-3M Line 2: A549 Line 3: HepG2)
WB (Western Blot)
(Western blot analysis of IRAK2 on different cell lysates using anti-IRAK2 at 1/500 dilution. Positive control�� Line 1: PC-3M Line 2: A549 Line 3: HepG2)
Related Product Information for anti-IRAK2 antibody
IRAK2 encodes the interleukin-1 receptor-associated kinase 2, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. IRAK2 is reported to participate in the IL1-induced upregulation of NF-kappaB. IRAK2 has been shown to interact with TRAF6 and Myd88.
Product Categories/Family for anti-IRAK2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
69,433 Da
NCBI Official Full Name
interleukin-1 receptor-associated kinase-like 2
NCBI Official Synonym Full Names
interleukin-1 receptor-associated kinase 2
NCBI Official Symbol
IRAK2
NCBI Official Synonym Symbols
IRAK-2
NCBI Protein Information
interleukin-1 receptor-associated kinase-like 2; interleukin-1 receptor associated kinase-2
UniProt Protein Name
Interleukin-1 receptor-associated kinase-like 2
UniProt Gene Name
IRAK2
UniProt Synonym Gene Names
IRAK-2
UniProt Entry Name
IRAK2_HUMAN
Similar Products
Product Notes
The IRAK2 irak2 (Catalog #AAA30491) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IRAK2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IRAK2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF),Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunoprecipitation (IP). WB: 1:500-1:2000 IHC: 1:50-1:200 ICC/IF: 1:50-1:200 IP: 1:10-1:50 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the IRAK2 irak2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IRAK2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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