WB (Western Blot)
(Detection of human IRF1 by western blot. Samples: Whole cell lysate (10 ug) from HeLa treated with interferon gamma, HeLa, MOLT-4, MCF-7, and RPMI-8226 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-IRF1 recombinant monoclonal antibody [BL-418-3E8] (AAA213542A lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
IRF1 recombinant antibody
Rabbit anti-IRF1 Recombinant Monoclonal Antibody [BLR039F]
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human IRF1 by western blot. Samples: Whole cell lysate (10 ug) from HeLa treated with interferon gamma, HeLa, MOLT-4, MCF-7, and RPMI-8226 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-IRF1 recombinant monoclonal antibody [BL-418-3E8] (AAA213542A lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot)
(Detection of human IRF1 by western blot. Samples: Whole cell lysate (10 ug) from HeLa treated with interferon gamma, HeLa, MOLT-4, MCF-7, and RPMI-8226 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-IRF1 recombinant monoclonal antibody [BL-418-3E8] (AAA213542A lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
IP (Immunoprecipitation)
(Detection of human IRF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-IRF1 recombinant monoclonal antibody [BL-418-3E8] (AAA213542A lot 2) used for IP at 20 ul/mg lysate. IRF1 was also immunoprecipitated by a previous lot of this antibody (AAA213542A lot 1) and a second antibody against a different epitope of IRF1 (BL428-1A9). For blotting immunoprecipitated IRF1, AAA213542A was used at 1:1000. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IP (Immunoprecipitation)
(Detection of human IRF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-IRF1 recombinant monoclonal antibody [BL-418-3E8] (AAA213542A lot 2) used for IP at 20 ul/mg lysate. IRF1 was also immunoprecipitated by a previous lot of this antibody (AAA213542A lot 1) and a second antibody against a different epitope of IRF1 (BL428-1A9). For blotting immunoprecipitated IRF1, AAA213542A was used at 1:1000. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IHC (Immunohistochemisry)
(Detection of human IRF1 by immunohistochemistry. Sample: FFPE section of ovarian carcinoma. Antibody: Rabbit anti-IRF1 recombinant monoclonal antibody (AAA213542-2). Secondary: HRP-conjugated goat anti-rabbit IgG .)
IHC (Immunohistochemisry)
(Detection of human IRF1 by immunohistochemistry. Sample: FFPE section of ovarian carcinoma. Antibody: Rabbit anti-IRF1 recombinant monoclonal antibody (AAA213542-2). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry)
(Detection of human IRF1 by immunocytochemistry. Sample: FFPE section of MJ cells. Antibody: Rabbit anti-IRF1 recombinant monoclonal antibody (AAA213542-2). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry)
(Detection of human IRF1 by immunocytochemistry. Sample: FFPE section of MJ cells. Antibody: Rabbit anti-IRF1 recombinant monoclonal antibody (AAA213542-2). Secondary: HRP-conjugated goat anti-rabbit IgG .)
FCM/FACS (Flow Cytometry)
(Detection of human IRF1 (shaded) in MOLT4 cells by flow cytometry. Antibody: Rabbit anti-IRF1 recombinant monoclonal (AAA213542) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
FCM/FACS (Flow Cytometry)
(Detection of human IRF1 (shaded) in MOLT4 cells by flow cytometry. Antibody: Rabbit anti-IRF1 recombinant monoclonal (AAA213542) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
NCBI and Uniprot Product Information
Similar Products
Product Notes
The IRF1 irf1 (Catalog #AAA213542) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-IRF1 Recombinant Monoclonal Antibody [BLR039F] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IRF1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the IRF1 irf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IRF1, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.