IF (Immunofluorescence)
(Immunofluorescent analysis of IRF7 staining in HEK293 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.)
Rabbit anti-Human, Rat IRF7 Monoclonal Antibody | anti-IRF7 antibody
Anti-IRF7 Antibody
Gene Names
IRF7; IRF7A; IRF7B; IRF7C; IRF7H; IRF-7H
Reactivity
Human, Rat
Applications
Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
IRF7, Antibody; Anti-IRF7 Antibody; anti-IRF7 antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Monoclonal
Specificity
Recognizes endogenous levels of IRF7 protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 50mM Tris-Glycine (pH7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.
Applicable Applications for anti-IRF7 antibody
IP (Immunoprecipitation), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide of human IRF7
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
IF (Immunofluorescence)
(Immunofluorescent analysis of IRF7 staining in HEK293 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.)
IF (Immunofluorescence)
(Immunofluorescent analysis of IRF7 staining in HEK293 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of IRF7 staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.19). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of IRF7 staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.19). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
WB (Western Blot)
(Western blot analysis of IRF7 expression in rat brain (A) whole cell lysates. (Predicted band size: 54 kD; Observed band size: 54 kD))
WB (Western Blot)
(Western blot analysis of IRF7 expression in rat brain (A) whole cell lysates. (Predicted band size: 54 kD; Observed band size: 54 kD))
Related Product Information for anti-IRF7 antibody
Rabbit monoclonal antibody to IRF7
Product Categories/Family for anti-IRF7 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
51-56kDa
NCBI Official Full Name
interferon regulatory factor 7 isoform a
NCBI Official Synonym Full Names
interferon regulatory factor 7
NCBI Official Symbol
IRF7
NCBI Official Synonym Symbols
IRF7A; IRF7B; IRF7C; IRF7H; IRF-7H
NCBI Protein Information
interferon regulatory factor 7; IRF-7; interferon regulatory factor-7H
UniProt Protein Name
Interferon regulatory factor 7
UniProt Gene Name
IRF7
UniProt Synonym Gene Names
IRF-7
UniProt Entry Name
IRF7_HUMAN
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Product Notes
The IRF7 irf7 (Catalog #AAA265698) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-IRF7 Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's IRF7 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the IRF7 irf7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IRF7, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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