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FCM/FACS (Flow Cytometry)
(ITA6 Monoclonal Antibody flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.)
Mouse anti-Human ITA6 Monoclonal Antibody | anti-ITGA6 antibody
ITA6 Antibody
Gene Names
ITGA6; CD49f; VLA-6; ITGA6B
Reactivity
Human
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Western Blot, ELISA, Immunofluorescence
Purity
Purified Mouse Monoclonal Antibody (Mab)
Synonyms
ITA6, Antibody; ITA6 Antibody; Integrin alpha-6; CD49 antigen-like family member F; VLA-6; CD49f; Integrin alpha-6 heavy chain; Integrin alpha-6 light chain; Processed integrin alpha-6; Alpha6p; ITGA6; anti-ITGA6 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,kappa
Clone Number
129CT49.6.5
Specificity
ITA6 recombinant protein is used to produce this monoclonal antibody.
Purity/Purification
Purified Mouse Monoclonal Antibody (Mab)
Form/Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Concentration
Vial Concentration: 0.42 (varies by lot)
Sequence Length
1073
Applicable Applications for anti-ITGA6 antibody
IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA, IF (Immunofluorescence)
Antigen Type
Recombinant Protein
Antigen Source
HUMAN
Cellular Location
Cell membrane; Single-pass type I membrane protein. Cell membrane; Lipid-anchor
Tissue Location
Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney).
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(ITA6 Monoclonal Antibody flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(ITA6 Monoclonal Antibody flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of ITA6 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of ITA6 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).)
IHC (Immunohiostchemistry)
(ITA6 Monoclonal Antibody (Cat. #AAA283472) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conj?gation of the secondary antibody and DAB staining. This data demonstrates the use of the ITA6 Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(ITA6 Monoclonal Antibody (Cat. #AAA283472) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conj?gation of the secondary antibody and DAB staining. This data demonstrates the use of the ITA6 Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(Anti-ITA6 at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 127 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-ITA6 at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 127 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-ITGA6 antibody
The ITGA6 protein product is the integrin alpha chain alpha 6. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. Two transcript variants encoding different isoforms have been found for this gene.
Product Categories/Family for anti-ITGA6 antibody
References
Evaluation of candidate stromal epithelial cross-talk genes identifies association between risk of serous ovarian cancer and TERT, a cancer susceptibility hot-spot. Johnatty SE, et al. PLoS Genet, 2010 Jul 8. PMID 20628624. Prostate cancer risk-associated variants reported from genome-wide association studies: Meta-analysis and their contribution to genetic Variation. Kim ST, et al. Prostate, 2010 Jun 16. PMID 20564319. alpha6-integrin subunit plays a major role in the proangiogenic properties of endothelial progenitor cells. Bouvard C, et al. Arterioscler Thromb Vasc Biol, 2010 Aug. PMID 20508204. Common polymorphisms in ITGA2, PON1 and THBS2 are associated with coronary atherosclerosis in a candidate gene association study of the Chinese Han population. Wang Y, et al. J Hum Genet, 2010 Aug. PMID 20485444. Usage of heparan sulfate, integrins, and FAK in HPV16 infection. Abban CY, et al. Virology, 2010 Jul 20. PMID 20441998.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
126606
NCBI Official Full Name
integrin alpha-6 isoform b
NCBI Official Synonym Full Names
integrin, alpha 6
NCBI Official Symbol
ITGA6
NCBI Official Synonym Symbols
CD49f; VLA-6; ITGA6B
NCBI Protein Information
integrin alpha-6
UniProt Protein Name
Integrin alpha-6
UniProt Gene Name
ITGA6
UniProt Synonym Gene Names
Alpha6p
UniProt Entry Name
ITA6_HUMAN
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Product Notes
The ITGA6 itga6 (Catalog #AAA283472) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ITA6 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ITA6 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA, IF (Immunofluorescence). Researchers should empirically determine the suitability of the ITGA6 itga6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ITA6, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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