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FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with Phospho-JNK1/2/3 (T183+T183+T221) antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Rabbit JNK1/2/3 Monoclonal Antibody | anti-JNK1/2/3 antibody
JNK1/2/3 (Phospho-T183+T183+T221) Antibody
Gene Names
MAPK8; JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
JNK1/2/3; Monoclonal Antibody; JNK1/2/3 (Phospho-T183+T183+T221) Antibody; C Jun kinase 2 antibody; c Jun N terminal kinase 1 antibody; c Jun N terminal kinase 2 antibody; c Jun N terminal kinase 3 antibody; c-Jun N-terminal kinase 1 antibody; JNK 46 antibody; JNK 55 antibody; JNK antibody; JNK-46 antibody; JNK1 antibody; JNK1A2 antibody; JNK2 antibody; JNK21B1/2 antibody; JNK2A antibody; JNK2ALPHA antibody; JNK2B antibody; JNK2BETA antibody; JNK3 alpha protein kinase antibody; JNK3 antibody; JNK3A antibody; Jun kinase antibody; JUN N terminal kinase antibody; MAP kinase 10 antibody; MAP kinase 8 antibody; MAP kinase 9 antibody; MAP kinase p49 3F12 antibody; MAPK 10 antibody; MAPK 8 antibody; MAPK 9 antibody; MAPK10 antibody; mapk8 antibody; MAPK9 antibody; Mitogen activated protein kinase 10 antibody; Mitogen activated protein kinase 8 antibody; Mitogen activated protein kinase 8 isoform JNK1 alpha1 antibody; Mitogen activated protein kinase 8 isoform JNK1 beta2 antibody; Mitogen activated protein kinase 9 antibody; Mitogen-activated protein kinase 8 antibody; MK08_HUMAN antibody; p493F12 antibody; p54a antibody; p54aSAPK antibody; p54bSAPK antibody; PRKM10 antibody; PRKM8 antibody; PRKM9 antibody; SAPK antibody; SAPK (beta) antibody; SAPK1 antibody; SAPK1a antibody; SAPK1b antibody; SAPK1c antibody; Stress activated protein kinase 1 antibody; Stress activated protein kinase 1a antibody; Stress activated protein kinase 1b antibody; Stress activated protein kinase 1c antibody; Stress activated protein kinase beta antibody; Stress activated protein kinase JNK1 antibody; Stress activated protein kinase JNK2 antibody; Stress activated protein kinase JNK3 antibody; Stress-activated protein kinase 1c antibody; Stress-activated protein kinase JNK1 antibody; anti-JNK1/2/3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
ST500
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-JNK1/2/3 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000-1:2000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Thr183 + Thr183 + Thr221 of human JNK1/2/3
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with Phospho-JNK1/2/3 (T183+T183+T221) antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with Phospho-JNK1/2/3 (T183+T183+T221) antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry)
(ICC staining Phospho-JNK1/2/3 (T183+T183+T221) in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Phospho-JNK1/2/3 (T183+T183+T221) in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Phospho-JNK1/2/3 (T183+T183+T221) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Phospho-JNK1/2/3 (T183+T183+T221) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Phospho-JNK1/2/3 (T183+T183+T221) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Phospho-JNK1/2/3 (T183+T183+T221) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Phospho-JNK1/2/3 (T183+T183+T221) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Phospho-JNK1/2/3 (T183+T183+T221) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Phospho-JNK1/2/3 (T183+T183+T221) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Phospho-JNK1/2/3 (T183+T183+T221) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Phospho-JNK1/2/3 (T183+T183+T221) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Phospho-JNK1/2/3 (T183+T183+T221) antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of Phospho-JNK1/2/3 (T183+T183+T221) on different lysates using anti-Phospho-JNK1/2/3 (T183+T183+T221) antibody at 1/1, 000 dilution. Positive control: Lane 1: NIH/3T3 cell lysate, treated with Anisomycin Lane 2: NIH/3T3 cell lysate, untreated)
WB (Western Blot)
(Western blot analysis of Phospho-JNK1/2/3 (T183+T183+T221) on different lysates using anti-Phospho-JNK1/2/3 (T183+T183+T221) antibody at 1/1, 000 dilution. Positive control: Lane 1: NIH/3T3 cell lysate, treated with Anisomycin Lane 2: NIH/3T3 cell lysate, untreated)
Related Product Information for anti-JNK1/2/3 antibody
JNKs (c-Jun N-terminal kinases) belong to a family of MAP kinases that are involved in a variety of cellular processes, including transcriptional regulation and cellular proliferation, differentiation and development. JNK2 (c-Jun N-terminal kinase 2) and JNK3 (c-Jun N-terminal kinase 3) are 424 and 464 amino acid proteins, respectively, that each contain one protein kinase domain and use magnesium as a cofactor to catalyze the phosphorylation of target proteins, thereby playing a role in a variety of events throughout the cell. Both JNK2 and JNK3 exist as multiple alternatively spliced isoforms and are subject to post-translational phosphorylation on Thr 183 and Thr 221, respectively, an event which activates JNK2/JNK3 enzymatic activity. Defects in the gene encoding JNK3 are a cause of epileptic encephalopathy of the Lennox-Gastaut type, a group of epileptic disorders characterized by severe psychomotor delay and seizures.
Product Categories/Family for anti-JNK1/2/3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
427
NCBI Official Full Name
Mitogen-activated protein kinase 8
NCBI Official Synonym Full Names
mitogen-activated protein kinase 8
NCBI Official Symbol
MAPK8
NCBI Official Synonym Symbols
JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2
NCBI Protein Information
mitogen-activated protein kinase 8; MAP kinase 8; JUN N-terminal kinase; c-Jun N-terminal kinase 1; stress-activated protein kinase 1; stress-activated protein kinase 1c; mitogen-activated protein kinase 8 isoform JNK1 beta2; mitogen-activated protein kin
UniProt Protein Name
Mitogen-activated protein kinase 8
UniProt Gene Name
MAPK8
UniProt Synonym Gene Names
JNK1; PRKM8; SAPK1; SAPK1C; MAP kinase 8; MAPK 8; SAPK1c
UniProt Entry Name
MK08_HUMAN
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Product Notes
The JNK1/2/3 mapk8 (Catalog #AAA29780) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The JNK1/2/3 (Phospho-T183+T183+T221) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's JNK1/2/3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). WB: 1:1000-1:2000 IHC: 1:50-1:200 ICC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the JNK1/2/3 mapk8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JNK1/2/3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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