IP (Immunoprecipitation)
(KLK11 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate1 uL anti-KLK11 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-KLK11 rabbit monoclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 31 kDaObserved band size: 49 kDa)
Rabbit anti-Human Kallikrein 11 Monoclonal Antibody | anti-KLK11 antibody
Recombinant Anti-Kallikrein 11 Antibody, Rabbit Monoclonal
Gene Names
KLK11; TLSP; PRSS20
Reactivity
Human
Applications
Immunoprecipitation, ELISA, Western Blot
Purity
Protein A
Synonyms
Kallikrein 11, Antibody; Recombinant Anti-Kallikrein 11 Antibody, Rabbit Monoclonal; KLK-11/Kallikrein-11 Antibody, Rabbit MAb; kallikrein-related peptidase 11; Anti-KLK11 Antibody; Anti-PRSS20 Antibody; Anti-TLSP Antibody; anti-KLK11 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
121
Specificity
Human KLK-11/Kallikrein 11
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-KLK11 antibody
IP (Immunoprecipitation), ELISA, WB (Western Blot)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Human KLK-11 protein
Conjugation
Unconjugated
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human KLK11/Kallikrein 11 (rh KLK-11).
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(KLK11 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate1 uL anti-KLK11 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-KLK11 rabbit monoclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 31 kDaObserved band size: 49 kDa)
IP (Immunoprecipitation)
(KLK11 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate1 uL anti-KLK11 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-KLK11 rabbit monoclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 31 kDaObserved band size: 49 kDa)
WB (Western Blot)
(Anti-KLK11 rabbit monoclonal antibody at 1:500 dilutionLane A: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:31 kDaObserved band size:42 kDa)
WB (Western Blot)
(Anti-KLK11 rabbit monoclonal antibody at 1:500 dilutionLane A: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:31 kDaObserved band size:42 kDa)
Related Product Information for anti-KLK11 antibody
kallikrein-related peptidase 11 (KLK11), also known as hippostasin, trypsin-like serine protease, and PRSS2, is a member of the human tissue kallikrein family. It is a subgroup of serine proteases with diverse physiological functions, which are implicated in carcinogenesis and some with potential that serving as novel biomarkers for ovarian and prostate cancer and other diseases. The KLK11 gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Two alternatively spliced forms exist, resulting in 25 (isoform 1) and 282 (isoform 2) amino acid sequences. Isoform 2 is identical to isoform 1, except for an inserted 32 amino acid segment. Isoform 1 is predominantly expressed in the brain whereas isoform 2 is preferentially expressed in the prostate.
References
Yoshida S., et al., (1998), cDNA cloning and expression of a novel serine protease, TLSP. Biochim. Biophys. Acta 1399:225-228.Mitsui S., et al., (2000), A novel isoform of a kallikrein-like protease, TLSP/hippostasin, (PRSS20), is expressed in the human brain and prostate.Biochem. Biophys. Res. Commun. 272:205-211.Yousef G.M., et al., (2000), Genomic organization, mapping, tissue expression, and hormonal regulation of trypsin-like serine protease (TLSP PRSS20), a new member of the human kallikrein gene family.Genomics 63:88-96.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,166 Da
NCBI Official Full Name
kallikrein-11 isoform 1
NCBI Official Synonym Full Names
kallikrein-related peptidase 11
NCBI Official Symbol
KLK11
NCBI Official Synonym Symbols
TLSP; PRSS20
NCBI Protein Information
kallikrein-11; hK11; hippostasin; kallikrein 11; serine protease 20; trypsin-like protease
UniProt Protein Name
Kallikrein-11
UniProt Gene Name
KLK11
UniProt Synonym Gene Names
PRSS20; TLSP; hK11
UniProt Entry Name
KLK11_HUMAN
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Product Notes
The KLK11 klk11 (Catalog #AAA255151) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-Kallikrein 11 Antibody, Rabbit Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Kallikrein 11 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the KLK11 klk11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Kallikrein 11, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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