FCM/FACS (Flow Cytometry)
(FlowcytometryusingtheRatantibodykappalightchainsantibodyOX-12(AAA72478). Paraformaldehydefixedratsplenocytespermeabilizedwith0.5%Tritonwerestainedwithanti-unknownspecificityantibodyortherabbitIgGversionofOX-12(AAA72478,blueline)atadilutionof1:100for1hatRT.Afterwashing,theboundantibodywasdetectedusingagoatanti-rabbitIgGAlexaFluor488antibodyatadilutionof1:1000andcellsanalyzedusingaFACSCantoflow-cytometer.)
kappa light chains Recombinant Antibody
Anti-Rat antibody kappa light chains [OX-12], Mouse IgG2a, kappa
Reactivity
Rat
Applications
Western Blot, immunoassay, Radioimmunoassay, Immunoprecipitation, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
Purity
Protein A affinity purified
Synonyms
kappa light chains, Recombinant Antibody; Anti-Rat antibody kappa light chains [OX-12], Mouse IgG2a, kappa; Rat antibody kappa light chains; Anti-Rat antibody kappa light chains [OX-12]; kappa Lc; OX12; MRC OC-12; MRC OX12; kappa light chains recombinant antibody
Host
Mouse
Reactivity
Rat
Clonality
Monoclonal
Isotype
Mouse IgG2a kappa
Clone Number
OX-12
Specificity
OX-12 binds to rat antibody kappa light chains - has preference for IgKa allotype.
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for kappa light chains recombinant antibody
WB (Western Blot), RIA (Radioimmunoassay), IP (Immunoprecipitation), ELISA, IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), IF (Immunofluorescence)
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
FCM/FACS (Flow Cytometry)
(FlowcytometryusingtheRatantibodykappalightchainsantibodyOX-12(AAA72478). Paraformaldehydefixedratsplenocytespermeabilizedwith0.5%Tritonwerestainedwithanti-unknownspecificityantibodyortherabbitIgGversionofOX-12(AAA72478,blueline)atadilutionof1:100for1hatRT.Afterwashing,theboundantibodywasdetectedusingagoatanti-rabbitIgGAlexaFluor488antibodyatadilutionof1:1000andcellsanalyzedusingaFACSCantoflow-cytometer.)
FCM/FACS (Flow Cytometry)
(FlowcytometryusingtheRatantibodykappalightchainsantibodyOX-12(AAA72478). Paraformaldehydefixedratsplenocytespermeabilizedwith0.5%Tritonwerestainedwithanti-unknownspecificityantibodyortherabbitIgGversionofOX-12(AAA72478,blueline)atadilutionof1:100for1hatRT.Afterwashing,theboundantibodywasdetectedusingagoatanti-rabbitIgGAlexaFluor488antibodyatadilutionof1:1000andcellsanalyzedusingaFACSCantoflow-cytometer.)
IF (Immunofluorescence)
(Immunofluorescencestainingoffixedratsplenocyteswithanti-RatkappalightchainsantibodyOX-12(AAA72478). ImmunofluorescenceanalysisofparaformaldehydefixedratsplenocytesonShi-fixcover-slipsstainedwiththechimericrabbitIgGversionofOX-12(AAA72478)(1:100dilution)for1hfollowedbyAlexaFluor488secondaryantibody(1:1000dilution),showingmembranestaininginsubsetofcells.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAAA72478,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
IF (Immunofluorescence)
(Immunofluorescencestainingoffixedratsplenocyteswithanti-RatkappalightchainsantibodyOX-12(AAA72478). ImmunofluorescenceanalysisofparaformaldehydefixedratsplenocytesonShi-fixcover-slipsstainedwiththechimericrabbitIgGversionofOX-12(AAA72478)(1:100dilution)for1hfollowedbyAlexaFluor488secondaryantibody(1:1000dilution),showingmembranestaininginsubsetofcells.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAAA72478,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
WB (Western Blot)
(Western BlotusingAnti-RatantibodykappalightchainsantibodyOX-12(AAA72478). Ratspleentissuelysate(35ugproteininRIPAbuffer)wereresolvedonaSDSPAGEgelandblotswereprobedwiththechimericrabbitversionofOX-12(AAA72478)0.001ug/mlbeforedetectionusingananti-rabbitsecondaryantibody.Aprimaryincubationof1hwasusedandproteinwasdetectedbychemiluminescence.)
WB (Western Blot)
(Western BlotusingAnti-RatantibodykappalightchainsantibodyOX-12(AAA72478). Ratspleentissuelysate(35ugproteininRIPAbuffer)wereresolvedonaSDSPAGEgelandblotswereprobedwiththechimericrabbitversionofOX-12(AAA72478)0.001ug/mlbeforedetectionusingananti-rabbitsecondaryantibody.Aprimaryincubationof1hwasusedandproteinwasdetectedbychemiluminescence.)
Related Product Information for kappa light chains recombinant antibody
OX-12 was generated by immunizing BALB/c mice with purified rat F(ab')2.
References
Hunt & Fowler A repopulation assay for B and T lymphocyte stem cells employing radiation chimaeras. Cell Tiss Kinet 14:445-64 1981 N/A6973397
Note on publication:
Describes the use of OX-12 to label B lymphocytes.
Note on publication:
Describes the use of OX-12 to label B lymphocytes.
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Product Notes
The kappa light chains (Catalog #AAA72478) is a Recombinant Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Rat antibody kappa light chains [OX-12], Mouse IgG2a, kappa reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's kappa light chains can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), RIA (Radioimmunoassay), IP (Immunoprecipitation), ELISA, IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), IF (Immunofluorescence). Researchers should empirically determine the suitability of the kappa light chains for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "kappa light chains, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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