Mouse Ki-67 Monoclonal Antibody | anti-Ki-67 antibody

Ki-67 (Proliferating Cell Marker)

Cat. Num. AAA23909

• Gene Names: MKI67; KIA; MIB-; MIB-1; PPP1R105
• Reactivity: Human. Others not known.
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Ki-67, Antibody; Ki-67 (Proliferating Cell Marker); KI-67; Ki67; KI-67 Antigen (KIA); MKI67; Proliferation related Ki-67 antigen; anti-Ki-67 antibody

• Host: Mouse
• Reactivity: Human. Others not known.
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Clone Number: MKI67/2466
• Specificity: Ki-67 antigen is a nuclear, non-histone protein that is present in all stages of the cell cycle except G0. This characteristic makes Ki-67 an excellent marker for proliferating cells and is commonly used as one of the prognostic factors in cancer studies. A correlation has been demonstrated between Ki-67 index and the histo-pathological grade of neoplasms. Assessment of Ki-67 expression in renal and ureter tumors shows a correlation between tumor proliferation and disease progression, thus making it possible to differentiate high-risk patients. Ki-67 expression may also prove to be important for distinguishing between malignant and benign peripheral nerve sheath tumors. Ki-67 labeling index has been shown to be a prognostic marker in a number of neoplasms including grade II astrocytoma, oligodendroglioma, colon carcinoma, and breast carcinoma. In general, Ki-67 is a good marker of proliferating cell populations.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 2,896

• Applicable Applications for anti-Ki-67 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistology (Formalin-Fixed)
• Application Notes: FC/FACS: 1-2ug/million cells; (HeLa Cells trypsinised 0.25% for 2-4minutes; 2-4%PFA fixed); IF: 1-2ug/ml; Immunohistology (Formalin-Fixed): 1-2ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
• Immunogen: Recombinant fragment of human Ki67 protein (around aa 2293-2478) (exact sequence is proprietary)
• Positive Control: Any actively proliferating cells. Skin, tonsil or lymph node.
• Cellular Localization: Cytoplasmic
• Chromosome Location: 10q26.2
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C.; Antibody without azide - store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Application Data

(Analysis of Protein Array containing more than 19,000 full-length human proteins using Ki67 Mouse Monoclonal Antibody (MKI67/2466). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

FCM (Flow Cytometry)

(Flow Cytometric Analysis of HeLa Cells using Ki67 Mouse Monoclonal Antibody (MKI67/2466). Goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Tonsil stained with Ki67 Mouse Monoclonal Antibody (MKI67/2466).)

SDS-PAGE

(SDS-PAGE Analysis Purified Ki67 Mouse Monoclonal Antibody (MKI67/2466). Confirmation of Purity and Integrity of Antibody.)

IF (Immunofluorescence)

(Confocal Immunofluorescence image of HeLa cells using Ki67 Mouse Monoclonal Antibody (MKI67/2466) Green (CF488) and Phalloidin (Purple) is used to label the membranes)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Tonsil stained with Ki67 Mouse Monoclonal Antibody (MKI67/2466).)

References

Gerdes J, et al. 1984. J. Immunol. 133:1710.Schluter C, et al. 1993 J. Cell Biol. 123:513.

NCBI and Uniprot Product Information

• NCBI GI #: 225543215
• NCBI GeneID: 4288
• NCBI Accession #: NP_001139438.1
• NCBI GenBank Nucleotide #: NM_001145966.1
• UniProt Accession #: P46013; Q5VWH2
• Molecular Weight: 345kDa; 395kDa
• NCBI Official Full Name: proliferation marker protein Ki-67 isoform 2
• NCBI Official Synonym Full Names: marker of proliferation Ki-67
• NCBI Official Symbol: MKI67
• NCBI Official Synonym Symbols: KIA; MIB-; MIB-1; PPP1R105
• NCBI Protein Information: proliferation marker protein Ki-67
• UniProt Protein Name: Proliferation marker protein Ki-67
• UniProt Gene Name: MKI67
• UniProt Synonym Gene Names: Antigen KI-67Curated; Antigen Ki67Curated