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IHC (Immunohistochemistry)
(Detection of human Ki-67 (red) by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Rabbit anti-Ki-67 recombinant monoclonal antibody (AAA213537 lot 1) used at 1:500. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI (blue).)
Ki-67 recombinant antibody
Rabbit anti-Ki-67 Recombinant Monoclonal Antibody [BLR021E]
Gene Names
MKI67; KIA; MIB-; MIB-1; PPP1R105
Reactivity
Human
Applications
Immunofluorescence, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunohistochemistry, Immunocytochemistry, Immunohistochemistry
Purity
Purified
Synonyms
Ki-67, Recombinant Antibody; Rabbit anti-Ki-67 Recombinant Monoclonal Antibody [BLR021E]; PPP1R105; antigen identified by monoclonal antibody Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; proliferation marker protein Ki-67; MIB-1; MIB-; KIA; antigen Ki67; Antigen KI-67; marker of proliferation Ki-67; Ki-67 recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BLR021E
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
50ug/ml (varies by lot)
Applicable Applications for Ki-67 recombinant antibody
mIF (Multipleximmunofluorescence), FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), IHC (Immunohistochemistry), ICC (Immunocytochemistry), IHC (Immunohistochemistry)
Immunogen
Between 1200 and 1250
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
IHC (Immunohistochemistry)
(Detection of human Ki-67 (red) by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Rabbit anti-Ki-67 recombinant monoclonal antibody (AAA213537 lot 1) used at 1:500. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI (blue).)
IHC (Immunohistochemistry)
(Detection of human Ki-67 (red) by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Rabbit anti-Ki-67 recombinant monoclonal antibody (AAA213537 lot 1) used at 1:500. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI (blue).)
IHC (Immunohistochemistry)
(Detection of human Ki-67 by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-Ki-67 recombinant monoclonal antibody (AAA213537 lot 3). Secondary: HRP-conjugated goat anti-rabbit IgG .)
IHC (Immunohistochemistry)
(Detection of human Ki-67 by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-Ki-67 recombinant monoclonal antibody (AAA213537 lot 3). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry)
(Detection of human Ki-67 by immunocytochemistry. Sample: acetone/methanol fixed Hela cells. Antibody: Rabbit anti-Ki-67 recombinant monoclonal antibody (AAA213537 lot 1) used at 1:100. Secondary: DyLight 594-conjugated goat anti-rabbit IgG . Counterstain: DAPI.)
ICC (Immunocytochemistry)
(Detection of human Ki-67 by immunocytochemistry. Sample: acetone/methanol fixed Hela cells. Antibody: Rabbit anti-Ki-67 recombinant monoclonal antibody (AAA213537 lot 1) used at 1:100. Secondary: DyLight 594-conjugated goat anti-rabbit IgG . Counterstain: DAPI.)
ICC (Immunocytochemistry)
(Detection of human Ki-67 by immunocytochemistry. Sample: FFPE section of MOLT-4 cells. Antibody: Rabbit anti-Ki-67 recombinant monoclonal antibody (AAA213537 lot 3). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry)
(Detection of human Ki-67 by immunocytochemistry. Sample: FFPE section of MOLT-4 cells. Antibody: Rabbit anti-Ki-67 recombinant monoclonal antibody (AAA213537 lot 3). Secondary: HRP-conjugated goat anti-rabbit IgG .)
FCM/FACS (Flow Cytometry)
(Detection of human Ki-67 (shaded) in MOLT4 cells by flow cytometry. Antibody: Rabbit anti-Ki-67 recombinant monoclonal (AAA213537 lot 1) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
FCM/FACS (Flow Cytometry)
(Detection of human Ki-67 (shaded) in MOLT4 cells by flow cytometry. Antibody: Rabbit anti-Ki-67 recombinant monoclonal (AAA213537 lot 1) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
Related Product Information for Ki-67 recombinant antibody
Ki-67 is a marker of cell proliferation detected in cells that are in G1-, S-, G2-, and M-phase of the cell cycle and absent in cells in G0. Ki-67 is detected exclusively in the nucleus, and its detection by immunohistological methods has proven to have test and prognostic value in the study of a variety of human tumors. The specific cellular functions of Ki-67 have not been elucidated, but it is proposed to function in the organization and maintenance of the architecture of DNA and in the synthesis of ribosomes during mitosis.
Product Categories/Family for Ki-67 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
319,444 Da
NCBI Official Full Name
antigen KI-67 isoform 1
NCBI Official Synonym Full Names
marker of proliferation Ki-67
NCBI Official Symbol
MKI67
NCBI Official Synonym Symbols
KIA; MIB-; MIB-1; PPP1R105
NCBI Protein Information
antigen KI-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; antigen identified by monoclonal antibody Ki-67
UniProt Protein Name
Antigen KI-67
UniProt Gene Name
MKI67
UniProt Entry Name
KI67_HUMAN
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Product Notes
The Ki-67 mki67 (Catalog #AAA213537) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Ki-67 Recombinant Monoclonal Antibody [BLR021E] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Ki-67 can be used in a range of immunoassay formats including, but not limited to, mIF (Multipleximmunofluorescence), FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), IHC (Immunohistochemistry), ICC (Immunocytochemistry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the Ki-67 mki67 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ki-67, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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