FCM/FACS (Flow Cytometry)
(Flow cytometry analysis of KIR/CD158 in PBMC. The cell was stained at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).)
Mouse anti-Human KIR/CD158 Monoclonal Antibody | anti-KIR/CD158 antibody
KIR/CD158
Gene Names
KIR2DL5A; CD158F; KIR2DL5; KIR2DL5.1; KIR2DL5.3
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Western Blot, ELISA
Synonyms
KIR/CD158, Antibody; KIR/CD158; CD158F; CD158F1; KIR2DL5; KI2LA_HUMAN; anti-KIR/CD158 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b kappa
Clone Number
AT11C11
Form/Format
Liquid; In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
375
Applicable Applications for anti-KIR/CD158 antibody
FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA
Immunogen
Recombinant human KIR/CD158 (22-238aa) purified from E Coli.
Conjugation
Unconjugated
Preparation and Storage
Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.
FCM/FACS (Flow Cytometry)
(Flow cytometry analysis of KIR/CD158 in PBMC. The cell was stained at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).)
FCM/FACS (Flow Cytometry)
(Flow cytometry analysis of KIR/CD158 in PBMC. The cell was stained at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).)
WB (Western Blot)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KIR/CD158 antibody (1:10000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;Lane 1.: 293T cell lysate;Lane 2.: MCF7 cell lysate;Lane 3.: HepG2 cell lysate;Lane 4.: K562 cell lysate)
WB (Western Blot)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KIR/CD158 antibody (1:10000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;Lane 1.: 293T cell lysate;Lane 2.: MCF7 cell lysate;Lane 3.: HepG2 cell lysate;Lane 4.: K562 cell lysate)
Related Product Information for anti-KIR/CD158 antibody
KIR/CD158, also known as killer cell immunoglobulin-like receptor 2DL5A, is a type I transmembrane glycoprotein that belongs to the killer cell Ig-like receptor (KIR) family. It is detected on the cell surface as a monomer that, upon tyrosine phosphorylation, recruits the Srchomology region 2-containing protein tyrosine phosphatase-2, It is thus an inhibitory receptor gathering a combination of genetic, structural, and functional features unique among KIR, which suggests that it plays a specialized role in innate immunity.
References
Vilches, C., et al. (2000) J. Immunol. 164: 5797-5804. Elfishawi SM., et al. (2017) Hum. Immunol. 78 (11-12), 684-691.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Killer cell immunoglobulin-like receptor 2DL5A
NCBI Official Synonym Full Names
killer cell immunoglobulin like receptor, two Ig domains and long cytoplasmic tail 5A
NCBI Official Symbol
KIR2DL5A
NCBI Official Synonym Symbols
CD158F; KIR2DL5; KIR2DL5.1; KIR2DL5.3
NCBI Protein Information
killer cell immunoglobulin-like receptor 2DL5A
UniProt Protein Name
Killer cell immunoglobulin-like receptor 2DL5A
UniProt Gene Name
KIR2DL5A
UniProt Synonym Gene Names
CD158F; CD158F1; KIR2DL5
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Product Notes
The KIR/CD158 kir2dl5a (Catalog #AAA48284) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The KIR/CD158 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's KIR/CD158 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the KIR/CD158 kir2dl5a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KIR/CD158, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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