WB (Western Blot)
(Western Blot analysis of Rat Brain showing detection of ~45 kDa Kir6.1 protein using Mouse Anti-Kir6.1 Monoclonal Antibody, Clone S366-60 at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~45 kDa. Other Band(s): ~100 kDa.)
Mouse Kir6.1 Monoclonal Antibody | anti-Kir6.1 antibody
Kir6.1 Antibody, Clone S366-60: ATTO 488
Gene Names
Kcnj8; Kir6.1; UKATP1; uKATP-1
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Protein G Purified
Synonyms
Kir6.1, Antibody; Kir6.1 Antibody, Clone S366-60: ATTO 488; Mouse Anti-Rat Kir6.1 Monoclonal IgG2A; Kir6.1 Antibody: ATTO 488; ATP-sensitive inward rectifier potassium channel 8 Antibody; Potassium channel inwardly rectifying subfamily J member 8 Antibody; uKATP-1 Antibody; Kcnj8 Antibody; anti-Kir6.1 antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG2A
Clone Number
S366-60
Specificity
Detects ~45 kDa.
Purity/Purification
Protein G Purified
Form/Format
PBS pH 7.4, 50% Glycerol, 0.1% Sodium Azide
Concentration
1 mg/ml (varies by lot)
Sequence Length
424
Applicable Applications for anti-Kir6.1 antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen Species
Rat
Immunogen
Fusion protein amino acids 306-424 (Cytoplasmic C-terminus) of rat Kir6.1
Conjugate
ATTO 488
Cellular Localization
Membrane
Certificate of Analysis
A 1:100 dilution was sufficient for detection of Kir6.1 in 20 ug of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Product Notes
Conjugated primary antibodies can be good options or alternatives to secondary antibody's for detecting a target protein. The lab manufactures a conjugated antibody from a primary parent antibody by attaching (conjugating) an available tag such as a fluorophore or protein, allowing detection of the target protein without using a secondary antibody. Conjugated primary antibodies may potentially be used to replace primary/secondary antibody pairs in various applications, such as Enzyme-Linked Immunosorbent Assay (ELISA), Flow Cytometry, Fluorescence-activated cell sorting, Förster Resonance Energy Transfer (FRET). Immunocytochemistry, Immunofluorescence, Immunohistochemistry, or Western Blot. The suitability of applications for conjugates are user determined and we cannot guarantee that any antibody-conjugate combination has been tested for use in a particular application.
Please note: Conjugate tags may potentially bind in the antibody paratope, which can limit binding of the antibody to the antigen. Binding in the antibody paratope may affect the ability of the antibody to bind to the corresponding antigen (protein target) in various species and applications. There is no way to confirm or guarantee the location of the antibody tag as we cannot control the binding of the conjugate to the antibody. Researchers are responsible for evaluating antibody conjugates for the suitability of their applications.
Please note: Conjugate tags may potentially bind in the antibody paratope, which can limit binding of the antibody to the antigen. Binding in the antibody paratope may affect the ability of the antibody to bind to the corresponding antigen (protein target) in various species and applications. There is no way to confirm or guarantee the location of the antibody tag as we cannot control the binding of the conjugate to the antibody. Researchers are responsible for evaluating antibody conjugates for the suitability of their applications.
Preparation and Storage
Store at-20 degree C.
WB (Western Blot)
(Western Blot analysis of Rat Brain showing detection of ~45 kDa Kir6.1 protein using Mouse Anti-Kir6.1 Monoclonal Antibody, Clone S366-60 at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~45 kDa. Other Band(s): ~100 kDa.)
WB (Western Blot)
(Western Blot analysis of Rat Brain showing detection of ~45 kDa Kir6.1 protein using Mouse Anti-Kir6.1 Monoclonal Antibody, Clone S366-60 at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~45 kDa. Other Band(s): ~100 kDa.)
ICC (Immunocytochemistry)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Kir6.1 Monoclonal Antibody, Clone S366-60 at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Kir6.1 Antibody. (D) Composite.)
ICC (Immunocytochemistry)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Kir6.1 Monoclonal Antibody, Clone S366-60 at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Kir6.1 Antibody. (D) Composite.)
Related Product Information for anti-Kir6.1 antibody
Several different potassium channels are known to be involved with electrical signaling in the nervous system. One class is activated by depolarization whereas a second class is not. The latter are referred to as inwardly rectifying K+ channels, and they have a greater tendency to allow potassium to flow into the cell rather than out of it. This asymmetry in potassium ion conductance plays a key role in the excitability of muscle cells and neurons. The protein encoded by this gene is an integral membrane protein and member of the inward rectifier potassium channel family (1-3). This is predominantly detected in fetal and adult hearts, and defects can be associated with J-wave syndromes, a group of heart disorders characterized by early repolarization events (4).
Product Categories/Family for anti-Kir6.1 antibody
References
1. Zobel C., et al. (2003) J Physiol. 550: 365-372. 2. Panama B.K., McLerie M., and Lopatin A.N. (2007) Am J Physiol Heart Circ Physiol. 293: H3558-H3567. 3. Munoz V., et al. (2007) Heart Rhythm. 4(4): 487-496. 4. Aguilar-Bryan L., et al. (1998) Physiol Rev. 78(1): 227-245.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
ATP-sensitive inward rectifier potassium channel 8
NCBI Official Synonym Full Names
potassium inwardly-rectifying channel, subfamily J, member 8
NCBI Official Symbol
Kcnj8
NCBI Official Synonym Symbols
Kir6.1; UKATP1; uKATP-1
NCBI Protein Information
ATP-sensitive inward rectifier potassium channel 8
UniProt Protein Name
ATP-sensitive inward rectifier potassium channel 8
UniProt Gene Name
Kcnj8
UniProt Entry Name
KCNJ8_RAT
Similar Products
Product Notes
The Kir6.1 kcnj8 (Catalog #AAA253963) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Kir6.1 Antibody, Clone S366-60: ATTO 488 reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Kir6.1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the Kir6.1 kcnj8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Kir6.1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.