WB (Western Blot)
(Detection of human KLF4 by western blot. Samples: Whole cell lysate (25 ug) from DLD-1, PC-3, MCF-7, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-KLF4 recombinant monoclonal antibody (AAA213663 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
KLF4 recombinant antibody
Rabbit anti-KLF4 Recombinant Monoclonal Antibody [BLR250L]
Gene Names
KLF4; EZF; GKLF
Reactivity
Human, Mouse
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
KLF4, Recombinant Antibody; Rabbit anti-KLF4 Recombinant Monoclonal Antibody [BLR250L]; endothelial Kruppel-like zinc finger protein; Kruppel-like factor 4 (gut); Krueppel-like factor 4; gut-enriched krueppel-like factor; gut Kruppel-like factor; GKLF; epithelial zinc finger protein EZF; EZF; Kruppel like factor 4; KLF4 recombinant antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Monoclonal
Clone Number
BLR250L
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
Concentration
1000ug/ml (varies by lot)
Applicable Applications for KLF4 recombinant antibody
ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 75 and 125
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human KLF4 by western blot. Samples: Whole cell lysate (25 ug) from DLD-1, PC-3, MCF-7, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-KLF4 recombinant monoclonal antibody (AAA213663 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot)
(Detection of human KLF4 by western blot. Samples: Whole cell lysate (25 ug) from DLD-1, PC-3, MCF-7, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-KLF4 recombinant monoclonal antibody (AAA213663 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot)
(Detection of mouse KLF4 by western blot. Samples: Whole cell lysate (25 ug) from CH27, CT26, C2C12, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-KLF4 recombinant monoclonal antibody (AAA213663 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot)
(Detection of mouse KLF4 by western blot. Samples: Whole cell lysate (25 ug) from CH27, CT26, C2C12, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-KLF4 recombinant monoclonal antibody (AAA213663 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
ICC (Immunocytochemistry)
(Detection of mouse KLF4 by immunocytochemistry. Sample: FFPE section of CT26 cells. Antibody: Rabbit anti-KLF4 recombinant monoclonal antibody (AAA213663 lot 1) used for IP at 6 ul/mg lysate. KLF4 was also immunoprecipitated by a second antibody against a different epitope of KLF4 (BL-2275B-1E8). For blotting immunoprecipitated KLF4, AAA213663 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
ICC (Immunocytochemistry)
(Detection of mouse KLF4 by immunocytochemistry. Sample: FFPE section of CT26 cells. Antibody: Rabbit anti-KLF4 recombinant monoclonal antibody (AAA213663 lot 1) used for IP at 6 ul/mg lysate. KLF4 was also immunoprecipitated by a second antibody against a different epitope of KLF4 (BL-2275B-1E8). For blotting immunoprecipitated KLF4, AAA213663 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for KLF4 recombinant antibody
KLF4 belongs to the Kruppel family of transcription factors. This zinc finger protein is required for normal development of the barrier function of skin. KLF4 is thought to control the G1-to-S transition of the cell cycle following DNA damage by mediating the tumor suppressor gene p53. [taken from NCBI Entrez Gene (Gene ID: 9314)].
Product Categories/Family for KLF4 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
51kDa
NCBI Official Full Name
Krueppel-like factor 4
NCBI Official Synonym Full Names
Kruppel-like factor 4 (gut)
NCBI Official Symbol
KLF4
NCBI Official Synonym Symbols
EZF; GKLF
NCBI Protein Information
Krueppel-like factor 4; gut-enriched krueppel-like factor; epithelial zinc finger protein EZF; endothelial Kruppel-like zinc finger protein
UniProt Protein Name
Krueppel-like factor 4
UniProt Gene Name
KLF4
UniProt Synonym Gene Names
EZF; GKLF
UniProt Entry Name
KLF4_HUMAN
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Product Notes
The KLF4 klf4 (Catalog #AAA213663) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-KLF4 Recombinant Monoclonal Antibody [BLR250L] reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's KLF4 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the KLF4 klf4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KLF4, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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